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Molecularly imprinted electrochemical sensor of electric copper-based MOFs sensitive membrane modified electrode and preparation method and detection method thereof

A technology of molecular imprinting and modifying electrodes, applied in the field of electrochemical sensing, can solve the problems of unclear morphology, unclear crystal form, and no clear elaboration of influence, etc., and achieve the effects of good conductivity, time saving and high sensitivity.

Active Publication Date: 2021-06-04
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the synthesis of MOFs sensitive membranes by electrochemical reduction method for modifying electrodes to prepare molecularly imprinted electrochemical sensors.
The effect of the ligands used on the properties (e.g., morphology, electrochemical properties, etc.) of electrochemically synthesized MOFs and their application in combination with molecular imprinting techniques is also not clearly addressed in the literature.
Moreover, the stability changes (e.g., morphology, crystal form, etc.)

Method used

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  • Molecularly imprinted electrochemical sensor of electric copper-based MOFs sensitive membrane modified electrode and preparation method and detection method thereof
  • Molecularly imprinted electrochemical sensor of electric copper-based MOFs sensitive membrane modified electrode and preparation method and detection method thereof
  • Molecularly imprinted electrochemical sensor of electric copper-based MOFs sensitive membrane modified electrode and preparation method and detection method thereof

Examples

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Effect test

Embodiment 1

[0039] 1. A molecularly imprinted electrochemical sensor of an electric copper-based MOFs sensitive film modified electrode, comprising a working electrode, characterized in that, outside the working electrode, a Cu-MOF sensitive film, a chitosan film, and a crosslinking agent are sequentially connected The target protein insulating layer and the polypyrrole molecularly imprinted layer, the polypyrrole molecularly imprinted layer has target protein imprinted holes.

[0040] 2. The molecularly imprinted electrochemical sensor according to claim 1, wherein the working electrode is a glassy carbon electrode, and the electric copper-based MOF sensitive film is a modified electrode, which is a novel highly ordered porous material , has the advantages of large specific surface area, porosity and adjustable function. The surface film is formed by stacking 100nm MOFs, and the surface is relatively dense. The MOFs are directly electrodeposited on the conductive substrate through the e...

Embodiment 2

[0047] 1) Weigh 10mmol copper nitrate trihydrate (Cu(NO 3 ) 2 ·3H 2 O), 15mmol 1,3,5-trimellitic acid (H 3 BTC) and 10mmol triethylamine hydrochloride (Et 3 NHCl) was dissolved in 50 mL of N,N-dimethylformamide (DMF);

[0048] 2) Deposit Cu-MOF on the surface of glassy carbon electrode by electrodeposition method. The electrodeposition process adopts the constant potential mode, and the electrodeposition is -1.3V for 5 minutes. After the end, the electrode is quickly taken out of the deposition solution and dried for later use. The prepared electrode was named Cu-MOF / GCE.

[0049] In the present invention, the specific steps of preparing the molecularly imprinted electrochemical sensor of human immunoglobulin G are as follows:

[0050] 3) Weigh 3 μL of 0.25mg / mL (in 0.02mol / L acetic acid) chitosan solution and drop it on the surface of the electrode, dry it at room temperature and name it CS / Cu-MOF / GCE.

[0051] 4) Add 10 μL of 2.5% glutaraldehyde solution dropwise on ...

Embodiment 3

[0057] Detection based on the molecularly imprinted electrochemical sensor prepared in Example 2:

[0058] 1) Immerse the modified electrode to 50mmol / l[K 3 Fe(CN) 6 ] in 1mol / l KCl solution, and then perform electrochemical voltammetry scanning and AC impedance measurement on each modified electrode, and the corresponding cyclic voltammetry (CV) diagram and AC impedance (EIS) diagram can be obtained after scanning.

[0059] 2) The prepared electrochemical sensor was immersed in the human immunoglobulin G standard solution containing 0, 0.001, 0.01, 0.05, 0.1, 0.5, 1, 5 ng / mL, and measured by differential pulse voltammetry (DPV). The degree is plotted against the DPV response value.

[0060] 3) Lysozyme (Lysozyme), human serum albumin (Human Serum Albumin) and bovine serum albumin (bovine Serum Albumin) were selected as structural analogues of human immunoglobulin to determine the selectivity of MIP / CS / Cu-MOF / GCE.

[0061] as attached Figure 4 As shown, there is a good li...

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Abstract

The invention discloses a molecularly imprinted electrochemical sensor based on an electric copper-based MOFs sensitive membrane modified electrode and a preparation method and a detection method thereof, and belongs to the field of electrochemical sensing. The specific protein human immune globulin G (IgG) in serum is selected as a research object, and the novel high-sensitivity molecularly imprinted electrochemical sensor is successfully constructed by combining a nano composite material, a new imprinting method, a new template treatment thought and a new detection method. The preparation method comprises the following steps: synthesizing a copper-based MOFs modified electrode on the surface of a glassy carbon electrode by an electro-deposition method to improve the conductivity and increase the specific surface area, sequentially modifying chitosan and glutaraldehyde to provide attachment sites of IgG, directly forming a polymer film on the surface of the modified electrode through pyrrole electro-polymerization, and finally, eluting the template to obtain the molecularly imprinted polymer modified electrode. The molecularly imprinted electrochemical sensor is high in sensitivity and good in selectivity, and is successfully applied to detection of target protein in an actual sample.

Description

technical field [0001] The invention belongs to the field of electrochemical sensing, and in particular relates to a preparation method and a detection method of a molecularly imprinted electrochemical sensor for an electrode modified by an electric copper-based MOFs sensitive film. Background technique [0002] According to the chemical structure and mode of action of immunoglobulins, immunoglobulins are roughly divided into immunoglobulin A (IgA), immunoglobulin D (IgD), immunoglobulin E (IgE), immunoglobulin G (IgG) and immunoglobulin Globulin M (IgM). In the human body, immunoglobulin usually acts as an antibody, and plays a certain defense and protection role for the body in the process of binding to antigens and other pathogenic factors. Human immunoglobulin G (IgG) is the most important type of immunoglobulin in the human body. In addition, IgG is the only immunoglobulin that can cross the placenta and plays a crucial role in neonatal immunity against infection. Ig...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3277G01N27/48
Inventor 罗爱芹梁阿新侯慧鹏唐珊珊刘毅权刘宗坤
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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