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Oral soft capsule for pomfret

A technology of soft capsules and silver pomfrets, applied in capsule delivery, medical preparations of non-effective ingredients, bacterial antigen components, etc., can solve the problems of large consumption of vaccines, high feed dissolution rate, drug escape, etc., and achieve simple preparation process Easy to use, high immune protection rate, and obvious immune effect

Inactive Publication Date: 2021-06-08
NINGBO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First, the breeding stage is prone to outbreaks of various diseases, including bacterial diseases, viral diseases, parasitic diseases, etc., and it is very easy to die on a large scale, bringing incalculable losses to the breeding industry
The main prevention method for common fish diseases is to use broad-spectrum antibiotics. However, doing so will cause the discharged aquaculture wastewater to affect other organisms, resulting in the abuse of antibiotics. Second, in the previous basic research, we screened out some pathogenic bacteria from diseased fish. However, it was found that the method of injection administration would cause damage to the fish body and increase the risk of pathogenic microorganism invasion, and the soaking administration would consume a large amount of vaccine, and the cost was too high; third, some vaccines would be used in aquaculture. Probiotics are used to improve the physique of silver pomfret and enhance immunity. However, during the breeding process of silver pomfret, the average daily water change is 100%, and splashing probiotics has little effect, and cannot form a stable flora
All the above three active ingredients can be administered by mixing feed. However, due to its own biological characteristics, silver pomfret needs to eat artificial lump feed, which is difficult to ensure homogenization, drug dosage is not easy to control, and dissolved feeding If the rate is high, the drug will dissipate in large quantities in the water, which will affect the efficacy of the drug and fail to achieve the expected effect
Therefore, there is an urgent need to develop a kind of enteric-coated "soft capsule" that is beneficial to the feeding of silver pomfret. On the one hand, it can wrap the active ingredient, release the drug in the intestinal tract, reduce the stimulation to the stomach, and avoid affecting food intake; The choice is very picky. "Soft capsules" need to have a certain food-attracting effect, and the existing aquaculture technology cannot provide a reasonable solution to the above problems. Therefore, innovative technologies must be used to solve the problem of silver pomfret administration.

Method used

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  • Oral soft capsule for pomfret
  • Oral soft capsule for pomfret
  • Oral soft capsule for pomfret

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: preparation method of soft capsule wall material

[0015] (1) glue

[0016] Select high-quality gelatin, add it to distilled water at a ratio of 1:1 by mass, bathe in water at 40°C for 10 minutes, and stir while heating until the gelatin is completely melted.

[0017] (2) Preparation of food attractant

[0018] Weigh chilled jellyfish with half the amount of gelatin, umbrella diameter 20-50cm, thaw with fresh water in advance, remove fascia and impurities, beat at high speed with a pulverizer until homogenized. Pass the jellyfish slurry through an 80-mesh sieve to remove impurities, then add it to the prepared gelatin solution in (1), stir evenly and set aside.

[0019] (3) Plasticizer

[0020] The plasticizer is preferably glycerin. Weigh glycerin with the same quality as that of jellyfish, and add it to the solution described in (2) three times. Each time you add glycerin, you must fully stir for 3-5 minutes. After mixing evenly, add the remaining gly...

Embodiment 2

[0021] Embodiment 2: the preparation of core material part

[0022] (1) Inactivated vaccine solution

[0023] ① Obtain the antigen, order at least one of Photobacterium mermaid, Edwardsiella, Vibrio parahaemolyticus, and Vibrio anguillaris from the China Microorganism Collection Center, inoculate it on LB liquid medium, culture it at 28°C for 24 hours, and detect it with a hemocytometer Bacteria concentration up to 10 9 CFU / ml or when the OD600 detected by the spectrophotometer reaches 0.6 or more, the culture is terminated, and the expanded culture medium of the strain is obtained;

[0024] ②Add formaldehyde solution at a volume ratio of 0.5%, inactivate the expanded culture solution of the bacterial strain at room temperature for 24 hours, shake it 8-10 times during the period, and obtain the inactivated bacterial solution of the bacterial strain;

[0025] ③Safety test, sterility test for the inactivated vaccine: Take 80 μl of the above-mentioned inactivated vaccine and sp...

Embodiment 3

[0033] Embodiment 3: soft capsule effect test

[0034] ① Ingestion effect test

[0035] In the previous breeding process, the silver pomfret's food intake would drop significantly every time the drug was used, and the body would become thin and weak, resulting in certain death. Therefore, the effect can be evaluated by comparing the food intake after injection and using the oral capsule of the present invention.

[0036] 200 healthy silver pomfrets aged 60-70 days were selected and randomly divided into 2 groups. They were raised for 3 days before the experiment, and treated with an empty stomach for 1 day after the temporary raising. The selected pathogenic bacteria is Photobacterium mermaid preserved in the laboratory. Make inactivated bacteria liquid with the method for embodiment 2. Group 1 is an oral immunization group, and the medicinal solution wrapped in soft capsules is an inactivated vaccine. Group 2 is the injection immunization group. Each tail was injected wi...

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Abstract

The invention provides an oral soft capsule for pomfret. The wall material of the oral soft capsule comprises gelatin, a plasticizer, water and a phagostimulant, wherein the phagostimulant is jellyfish homogenate. The oral soft capsule for pomfret can delay the entrance of a medicine into a circulatory system, and the jellyfish homogenate component added in the wall material has a good food calling effect and can meet the requirement of pomfret. The immune protection rate of an inactivated vaccine against common bacterial diseases of pomfret is high. The oral slow-release preparation for pomfret can effectively avoid fish body injury caused by injection and medicine abuse caused by soaking. The oral soft capsule for pomfret has the characteristics of simple and feasible preparation process, low cost, obvious immune effect and the like.

Description

technical field [0001] The invention belongs to the technical field of preparations, and in particular relates to a silver pomfret oral soft capsule. Background technique [0002] Silver Pomfret (Pampus argenteus) belongs to Perciformes, Stromateidae, and Pampus. It is one of the important economic fishes in my country and is deeply loved by consumers. The annual market demand is about 28 billion yuan . [0003] In recent years, due to overfishing and environmental pollution, wild resources have been shrinking, and individuals of silver pomfret caught tend to be smaller and younger. Under the dual requirements of protecting fishery resources and meeting market demand, we started the work of artificial breeding of silver pomfret, and gradually broke through the bottleneck, going further and further on the road to industrialization. However, the problems exposed during the period should not be underestimated. First, the breeding stage is prone to outbreaks of various disease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/48A61K47/46A61K39/02A61K39/106A61K35/742A61K35/744A61K35/745A61K31/7036A61K31/545A61P31/04A61P37/04
CPCA61K9/4816A61K47/46A61K39/0208A61K39/025A61K39/107A61K35/742A61K35/744A61K35/745A61K31/7036A61K31/545A61P31/04A61P37/04A61K2039/521A61K2039/552A61K2039/542A61K2300/00
Inventor 张曼王亚军胡佳宝匡思雯顾玮玮孙弋博张友仪李亚亚杨阳
Owner NINGBO UNIV
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