Method for enhancing methane production through biogas fermentation by reinforcing carbon dioxide utilization through fermentation separation coupling integration

A technology of fermentation separation coupling and bio-natural gas, applied in the biological field, can solve the problems of increasing production costs, supporting equipment and high operating requirements

Pending Publication Date: 2021-06-08
DALIAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Therefore, most of the existing biological natural gas fermentation technologies are at the back end of the production equipment, supplemented by methane separation and purification, carbon dioxide removal and other equipment, of which carbon dioxide mainly relies on biological methods (algae and photosynthetic bacteria, etc.), microbial batteries, electrochemical, Photochemical, carbonation and other methods for ectopic removal, fixation or resource reuse [13] , higher requirements for supporting equipment and operation, and increased production costs

Method used

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  • Method for enhancing methane production through biogas fermentation by reinforcing carbon dioxide utilization through fermentation separation coupling integration

Examples

Experimental program
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Effect test

Embodiment 1

[0029] (1) Trichoderma reesei fermentation bacteria enzyme solution preparation:

[0030] The substratum composition that is used to prepare Trichoderma reesei fermentation bacteria enzyme liquid comprises: 10g / L glucose, 20g / L wheat bran, 1g / L peptone, 0.3g / L urea, 2.8g / L (NH 4 ) 2 SO 4 , 4g / L KH 2 PO 4 , 0.6g / L MgSO 4 ·7H 2 O, 0.8g / L CaCl 2 , 0.01g / L FeSO 4 ·7H 2 O, 0.004g / L MnSO 4 ·H 2 O, 0.03g / L ZnSO 4 ·7H 2 O, 0.005g / L CoCl 2 , 0.2mL / L Tween 80.

[0031] The above liquid medium was sterilized at 121° C. for 15 minutes, and after cooling to room temperature, Trichoderma reesei spore liquid (wild type or genetically engineered recombinant Trichoderma reesei) was inoculated in the medium at 10% by volume. 28°C, 400rpm, control pH 4.5, control dissolved oxygen 20%, control glucose concentration not higher than 0.5g / L, cultivate for 6 days, and then get wild-type Trichoderma reesei Rut C30 fermented enzyme liquid or high-yielding fiber Genetic engineering recomb...

Embodiment 2

[0036] Using corn stalks and duck manure as bio-natural gas fermentation materials, first add the wild-type Trichoderma reesei Rut C30 fermentation bacteria enzyme solution in the above-mentioned Example 1 according to the solid-to-liquid ratio of 1:8 of the stalks, and pretreat the stalks 5 after mixing evenly After 1 day, mix with duck manure again to obtain straw mixed solid waste; then add the Clostridium carbon monoxide fermented bacteria liquid in the above-mentioned embodiment 1 according to 15% of the quality of straw mixed solid waste, mix evenly and place it in a working volume of 3L Bio-natural gas fermentation is carried out in a solid-state fermentation reactor. In this embodiment, the solid-state fermentation reactor is equipped with a temperature control device, but there is no gas circulation and reflux device, that is, the carbon dioxide generated in this embodiment does not circulate and reflux to the fermentation system. Oxygen in the fermentation system was...

Embodiment 3

[0039]Using corn stalks and duck manure as bio-natural gas fermentation materials, first add the recombinant Trichoderma reesei (CGMCCNo. 12768) fermented bacteria enzyme liquid, mixed evenly and pretreated the stalks for 5 days, then mixed with duck manure to obtain straw mixed solid waste; then add the carbon monoxide shuttle in the above-mentioned embodiment 1 by 15% of the quality of the straw mixed solid waste Bacteria fermentation broth, mixed evenly and placed in a solid-state fermentation reactor with a working volume of 3L for bio-natural gas fermentation. In this embodiment, the solid-state fermentation reactor is equipped with a temperature control device, and the bottom of the reactor is equipped with a gas reflux device. After the fermentation gas is separated and purified by the gas separation membrane system, the remaining carbon dioxide can be recycled to the fermentation system (such as figure 1 shown). Before the bio-natural gas fermentation starts, nitrogen...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a method for enhancing methane production through biogas fermentation by reinforcing carbon dioxide utilization through fermentation separation coupling integration. According to the invention, solid wastes such as crop straws are used for biogas fermentation, and anaerobic bacterial strains capable of utilizing carbon dioxide are added to ferment bacterial liquid, so that fixation utilization and biotransformation of carbon dioxide in biogas fermentation are realized, methane synthesis is facilitated, and the yield and proportion of methane are increased; by coupling a fermentation reactor and a gas separation membrane system, the carbon dioxide circularly flows back to a fermentation system while methane is separated and purified, so that the in-situ fixation efficiency of the carbon dioxide is further improved; and straw materials are pretreated through trichoderma reesei zymophyte enzyme liquid, so that lignocellulose is rapidly degraded into fermentation monosaccharide, proliferation of carbon dioxide fixation utilization strains and biogas fermentation strains is promoted, and the in-situ fixation efficiency of the carbon dioxide and the performance of methane production through biogas fermentation are further improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for enhancing carbon dioxide fermentation, separation, coupling and integration to improve bio-natural gas fermentation to produce methane. Background technique [0002] my country is short of oil resources. In 2018, its oil import volume surpassed that of the United States and became the world's largest oil importer. In 2019, its oil import volume reached 506 million tons, a year-on-year increase of 9.55%, and its dependence on foreign oil was as high as 72%. Microbial fermentation has the advantages of "recycling, high-efficiency utilization, and clean production". It can catalyze the conversion of many biomass resources into renewable biomass energy. An important energy strategy for sustainable development. In fact, my country's annual agricultural straw production exceeds 900 million tons, and livestock breeding produces 3.8 billion tons of waste annually. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/12C12M1/107C12M1/00C12P5/02C12P19/14C12P19/02C12N1/14C12N1/20B01D53/84B01D53/62B01D53/22C02F11/04C12R1/885C12R1/145C12R1/02C12R1/01
CPCC12M21/04C12M29/02C12M47/18C12P5/023C12P19/14C12P19/02C12N1/14C12N1/20B01D53/84B01D53/62B01D53/229C02F11/04B01D2257/504B01D2251/95Y02E50/30Y02A50/20Y02W10/20Y02P20/59
Inventor 薛闯吴又多
Owner DALIAN UNIV OF TECH
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