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Anaerobic dehalogenation microbial agent and amplified production method thereof

A production method and technology of halogenated bacteria agent, applied in the field of actual site restoration of halogenated organic matter, can solve the problems of complex production operation of anaerobic dehalogenation bacteria, high anaerobic requirements of anaerobic dehalogenation bacteria, and difficulty in large-scale production. , to achieve the effect of low cost, improved efficiency and cost saving

Active Publication Date: 2021-07-23
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the defects and deficiencies of the existing anaerobic dehalogenation bacteria agents, which are complicated in production operation, small in yield, high in anaerobic requirements of anaerobic dehalogenation bacteria in the environment, and difficult to realize large-scale production. Provide a kind of scale-up production method of anaerobic dehalogenation agent

Method used

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  • Anaerobic dehalogenation microbial agent and amplified production method thereof
  • Anaerobic dehalogenation microbial agent and amplified production method thereof
  • Anaerobic dehalogenation microbial agent and amplified production method thereof

Examples

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Effect test

Embodiment 1

[0055] A kind of amplified production method of anaerobic dehalogenation bacteria agent, concrete steps are: select 20L polyethylene small-mouth plastic barrels, install the tap water that volume ratio is 98.92%, add the sodium bicarbonate of 30mM concentration successively as pH buffering agent, 360mg / L COD cassava flour (180mg / L COD) and sludge digestion supernatant (180mg / LCOD) mixture, the volume fraction is 0.08% anaerobic digestion sludge, then wrapped with a parafilm, and cultivated at room temperature for 2 weeks. Use gas chromatography (flame ion detector (FID), GasPro separation column) to monitor its methane production situation. When the methane concentration is not lower than 0.2mmol / L, it shows that the system is in strict anaerobic conditions, and the anaerobic reduction potential≦-200mV, At this time, 1 mmol / L trichlorethylene was added as the electron acceptor of the dehalogenated bacteria, and the enriched material of the dehalogenated bacteria was inoculated...

Embodiment 2

[0059] The method of this embodiment is the same as that of Embodiment 1, the difference is that the anaerobic dehalogenation bacteria are cultivated with the halogenated organic compound cis-dichloroethylene as the single variable. The result is as Figure 4 As shown, after 51 days of cultivation, cis-dichloroethylene was completely dechlorinated to form non-toxic ethylene. Also obtained the enriched anaerobic dehalogenating agent.

Embodiment 3

[0061] The method of this embodiment is the same as that of Embodiment 1, the difference is that the anaerobic dehalogenation bacteria are cultivated with the halogenated organic substance tetrachlorethylene as a single variable. The result is as Figure 5 As shown, after 35 days of cultivation, tetrachlorethylene was completely dechlorinated to form non-toxic ethylene. Also obtained the enriched anaerobic dehalogenating agent.

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Abstract

The invention discloses an anaerobic dehalogenation microbial agent and an amplified production method thereof. Anaerobic digestion sludge is inoculated, facultative anaerobes in the anaerobic digestion sludge are used for consuming oxygen left in a container and water, a system is in a strict anaerobic condition, and an ecological niche suitable for growth of dehalogenation bacteria is provided for subsequent inoculation of the dehalogenation bacteria; in addition, through a subsequent acetification process, a carbon source, an electron donor (acetate, hydrogen and the like) and the like needed by subsequent dehalogenation of the anaerobic dehalogenation bacteria can be provided. The method avoids the complex process of an anaerobic glove box or an anaerobic operation platform, is simpler and lower in cost and can produce the anaerobic dehalogenation microbial agent on a large scale, the amplified cultured dehalogenation microbial agent can achieve complete dehalogenation of tetrachloroethylene, trichloroethylene and cis-dichloroethylene and can dechlorinate PCBs, the production scale of the microbial agent can be adjusted according to the size of a polluted area, and a feasible solution is provided for microbial agent requirements of actual halogenated organic matter polluted site remediation.

Description

technical field [0001] The invention relates to the technical field of actual site restoration of halogenated organic matter, and more specifically, relates to an anaerobic dehalogenating bacteria agent and its enlarged production method. Background technique [0002] Halogenated organic compounds (Organohalide Compounds) are a large class of organic compounds that contain one or more halogen atoms (such as fluorine, chlorine and bromine) that are covalently bonded to carbon atoms in the organic structure, including the typical tetrachloro Ethylene (PCE), trichlorethylene (TCE), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs) and dioxins and furans (PCDD / Fs), etc. Trichlorethylene and tetrachlorethylene are often used as degreasing agents, organic solvents and chemical synthesis intermediates and are widely used in military industry, industry and agriculture. Halogenated organic compounds such as polychlorinated biphenyls and polybrominated diphenyl...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/36
CPCC12N1/20B09C1/10C02F3/34B09C2101/00C02F2101/36Y02E50/30
Inventor 汪善全吴日枫
Owner SUN YAT SEN UNIV
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