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Gene transport carrier of brain-targeting zinc oxide quantum dots modified by glutathione and preparation method of gene transport carrier

A glutathione and gene transport technology, applied in the field of medical materials, can solve problems such as autoimmune inflammation, gene mutation, etc.

Active Publication Date: 2021-09-24
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using a virus as a gene carrier may cause autoimmune reactions and inflammation, and there is even a risk of gene mutation

Method used

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  • Gene transport carrier of brain-targeting zinc oxide quantum dots modified by glutathione and preparation method of gene transport carrier
  • Gene transport carrier of brain-targeting zinc oxide quantum dots modified by glutathione and preparation method of gene transport carrier
  • Gene transport carrier of brain-targeting zinc oxide quantum dots modified by glutathione and preparation method of gene transport carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Embodiment 1 A kind of preparation of hydrophilic nanometer zinc oxide quantum dot

[0097] 1. Experimental method

[0098] 1. Preparation of nano zinc oxide by sol-gel method

[0099] 1.10g (5mmol) Zn (Ac) 2 2H 2 The O sample was dissolved in 50 mL of normal pressure boiling ethanol, and then cooled to 0 °C. A white powder is precipitated at near room temperature, which is anhydrous zinc acetate Zn(Ac) 2 .

[0100] Then 0.29g (7mmol) LiOH·H 2 O was dissolved in 50 mL of ethanol at room temperature and cooled to 0 °C in an ultrasonic bath. Under vigorous stirring at 0°C, the hydroxide-containing solution was added dropwise to the Zn(Ac) prepared above 2 in suspension. The reaction mixture became clear when about 0.1 g LiOH was added. Store the obtained nano-ZnO at ≦4°C or freeze-dry storage.

[0101] 2. Preparation of zinc methacrylate

[0102] Mix methacrylic acid and ultrapure water at a volume ratio of 1:4, and heat to 65°C to dissolve it. Add the prepare...

Embodiment 2

[0116] Example 2 UV-Vis Spectral Analysis of Water-Soluble Nano Zinc Oxide Core-Shell Quantum Dots

[0117]1. Experimental method

[0118] Take 2ml each of the ZnO@Polymer, ZnO@Polymer-Np and ZnO@Polymer-NpG (in terms of equal mass [Zn]) solutions prepared in Example 1 and place them in a quartz dish, while using deionized water as a blank, and then The absorption peak scanning was performed with a Unico 2802 ultraviolet spectrophotometer, and the wave band was set at 200nm-800nm.

[0119] 2. Experimental results

[0120] figure 2 are the UV-Vis absorption spectra of the three particles. It can be seen from the figure that ZnO@Polymer has an obvious exciton absorption peak around 330nm, and the absorption peak at 330nm still exists after NGF, pDNA and GSH modification, indicating that the particles after functional modification retain the ZnO core, and Well protected by a polymer shell. In addition, the characteristic absorption peak of glutathione appeared in ZnO@Polyme...

Embodiment 3

[0121] Embodiment 3 fluorescence spectrum analysis and photoluminescence detection

[0122] 1. Experimental method

[0123] Take 3ml each of the ZnO@Polymer, ZnO@Polymer-Np and ZnO@Polymer-NpG (based on equal mass [Zn]) solutions prepared in Example 1 and place them in a quartz fluorescence cuvette, and use deionization as a blank at the same time base. Then scan with HoribaJobinYvon fluoromax-4 fluorescence spectrometer. The excitation wavelength was set at 330 nm, and the range of absorption wavelength was set at 400 to 700 nm.

[0124] For photoluminescence detection, 3 to 4 mL of the ZnO@Polymer, ZnO@Polymer-N, ZnO@Polymer-Np and ZnO@Polymer-NpG solutions prepared in Example 1 were placed in a quartz cuvette, and then white light and Irradiate with ultraviolet light and record photos.

[0125] 2. Experimental results

[0126] The result is as image 3 as shown, image 3 a is the color result of the four particles under the irradiation of white light and ultraviolet ...

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Abstract

The invention discloses a gene transport carrier of brain-targeting zinc oxide quantum dots modified by glutathione and a preparation method of the gene transport carrier. The gene transport carrier can be successfully swallowed by cells and emits yellow fluorescence; can maximize activity of C6 and SH-SY5Y cells; and repair apoptosis caused by MPP+; can significantly reduce increase in ROS caused by MPP+ and return to normal levels; can significantly reduce protein expression level of alpha-synuclein, and immunofluorescence of tyrosine hydroxylase and aipha-syn also shows that the expression level is equivalent to that of a control group The gene transport carrier can increase neuron targeting and trophism, realizes synthesis interference on alpha-synuclein, can smoothly break through the blood brain barrier (BBB) after intravenous injection, successfully enters brain parenchyma and successfully restores functional damage of an in-vivo and in-vitro Parkinson's disease model, and is a promising gene transport carrier.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a glutathione-modified zinc oxide quantum dot gene delivery carrier targeting the brain. Background technique [0002] Parkinson's disease (PD) is a common degenerative disease of the nervous system in middle-aged and elderly people. Patients show slow movement, tremors in hands or feet and other parts of the body, loss of body flexibility, and muscle stiffness. Parkinson's disease has become another life-threatening killer after cardiovascular and cerebrovascular diseases and cancer. The main brain region affected in Parkinson's disease is L-dopamine degeneration in the nigrostriatum of the midbrain. [0003] The pathogenesis of Parkinson's disease is still unclear, and traditional treatment has great limitations. At present, there are mainly two traditional treatment methods for PD: 1) surgical treatment: reduce the dosage of drugs by transplanting dopaminergic neur...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K47/62A61K31/7088A61K31/713A61K38/18A61K47/32A61K49/00A61P25/16B82Y5/00
CPCA61K49/0019A61K49/0054A61K49/0056A61K49/0093A61K31/7088A61K31/713A61K38/185A61K47/6925A61K47/62A61K47/32A61P25/16B82Y5/00A61K2300/00Y02A50/30
Inventor 关燕清尹亮李明超林丹敏高逸飞
Owner SOUTH CHINA NORMAL UNIVERSITY
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