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Biodegradable complex microbial inoculant for aureomycin fungus residue waste and compost high-temperature pre-fermentation method of biodegradable complex microbial inoculant

A compound bacterial agent and biodegradation technology, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and fertilizers made from biological waste, can solve the problems of weak adaptability of aureomycin fermentation slag, and achieve the goal of shortening the time-consuming Harmful time, enhance vitality, and realize the effect of resource utilization

Active Publication Date: 2021-10-01
福建省固体废物处置有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the weak adaptability of single bacterial species to different chlortetracycline fermentation residues in the above-mentioned prior art, the present invention provides a biodegradable composite microbial agent of aureomycin residue waste, which includes natto spores Bacillus bacteria powder and Aspergillus niger powder, combined with Bacillus tequilas bacteria powder and / or Phanerochaete chrysosporium bacteria powder, the total number of viable bacteria in the composite bacteria agent is ≥1×10 8 cfu / g

Method used

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  • Biodegradable complex microbial inoculant for aureomycin fungus residue waste and compost high-temperature pre-fermentation method of biodegradable complex microbial inoculant
  • Biodegradable complex microbial inoculant for aureomycin fungus residue waste and compost high-temperature pre-fermentation method of biodegradable complex microbial inoculant
  • Biodegradable complex microbial inoculant for aureomycin fungus residue waste and compost high-temperature pre-fermentation method of biodegradable complex microbial inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1-test bacterial strain is to aureomycin tolerance

[0034] The strains of Bacillus natto, Bacillus tequilas, Bacillus tequilas, Phanerochaete chrysosporium, and Aspergillus niger were respectively inoculated into inorganic salt medium with different concentrations of aureomycin as carbon source, After shaking the flask at 30°C and 180rpm for 48 hours, observe the growth of the strain. The test results are shown in Table 1:

[0035] The growth situation of table 1 bacterial strain in the inorganic salt medium containing different concentrations of aureomycin

[0036]

[0037] Note: — indicates sterile growth, ++ indicates good growth, + indicates growth or slight inhibition

[0038] Among them, the composition of the inorganic salt medium is: NaCl 0.02%, dipotassium hydrogen phosphate 0.04%, potassium dihydrogen phosphate 0.04%, NH 4 NO 3 0.2%, magnesium sulfate 0.01%, manganese sulfate 0.001%, ferrous sulfate 0.001%, calcium chloride 0.01%, after ster...

Embodiment 2

[0040] The preparation of embodiment 2-bacterial strain bacterial powder

[0041] A. Preparation of Bacillus natto bacterium powder: Inoculate Bacillus natto into LB culture medium, culture in shake flask at 37°C and 180rpm for 24h, then centrifuge and dehydrate the obtained culture, and then freeze-dry in a vacuum to obtain each bacterial strain Freeze-dried powder, finally mix the freeze-dried powder with corn starch to make 1ⅹ10 8 cfu / g of bacteria powder;

[0042] B. Preparation of Bacillus tequilas bacteria powder: adopt the same preparation method as above-mentioned Bacillus natto bacteria powder to make 1ⅹ10 8 cfu / g of bacteria powder;

[0043] C. Preparation of Aspergillus niger powder: inoculate Aspergillus niger into solid bran medium (mixing bran and water at a ratio of 1:1) and culture at 37°C for 120 hours, after-cooking at room temperature for 48 hours, during which the koji is turned once a day; The cultured koji is mixed with bran, dried at 45-55°C for 24 ho...

Embodiment 3

[0045] Degradation test of bacterial residue solid waste after the extraction of aureomycin by the bacterial strain of embodiment 3

[0046] The bacteria powder prepared in Example 2 was respectively added to the solid waste of the bacteria residue after the extraction of aureomycin to ferment and test the degradation effect. The specific operation method is as follows:

[0047] Take aureomycin fermented and extracted fungus residue solid waste, water content is about 45%, pH is between 7-9, crushed to below 5mm, then add a certain amount of water to adjust the water content of fungus residue to about 60% , adjust the pH to about 7, pack in 70g / 500mL Erlenmeyer flasks, and aseptically sterilize. Then add 1 mg / g of sterile aureomycin in each conical flask, mix well, add the bacterial strain prepared in Example 2 with 5% inoculation amount, put in 37 ℃ of static culture 5d put bottle, simultaneously with The fermentation substrate without strain inoculation was used as a contro...

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Abstract

The invention relates to the field of microbial preparations, in particular to a biodegradable complex microbial inoculant for aureomycin fungus residue waste and a compost high-temperature pre-fermentation method of the biodegradable complex microbial inoculant for the aureomycin fungus residue waste. The biodegradable complex microbial inoculant for the aureomycin fungus residue waste comprises bacillus natto powder and aspergillus niger powder, bacillus tequilariae powder and / or phanerochaete chrysosporium powder are compounded, and the total viable count of the complex microbial inoculant is greater than or equal to 1 * 10 <8> cfu / g. According to the complex microbial inoculant, bacillus capable of tolerating aureomycin and mould are selected to form the complex microbial inoculant, aureomycin fungus residue waste added with a certain amount of bran is fermented, and the temperature of the fermentation process is rapidly increased through the synergistic effect of several strains capable of degrading aureomycin and decomposing protein, amino acid and a large number of carbon sources in thalli; the degradation of the aureomycin is accelerated. The fungus residues with lower aureomycin concentration are also beneficial to further growth and reproduction of the strains, and the synergistic fermentation is rapidly carried out, so that the fermentation and decomposition time is shortened, and the decomposition is more complete, thereby realizing resource utilization.

Description

technical field [0001] The invention relates to the field of microbial preparations, in particular to a biodegradable composite bacterial agent for aureomycin residue waste and a high-temperature pre-fermentation method for composting. Background technique [0002] Aureomycin is a broad-spectrum antibiotic produced by fermentation of Streptomyces. During the fermentation process, a large amount of aureomycin residue waste after solvent extraction is produced. These solid wastes mainly include microbial mycelium, culture Base residues, fermentation metabolites, filter aids and solvents, etc. Residual aureomycin degrades slowly under natural conditions, and it is easy to induce the production of drug-resistant bacteria in the environment, while the bacteria and culture medium residues contain crude protein, amino acids, a large amount of carbon sources and trace elements, etc., which are exposed to the environment The foul-smelling substances that are easily corrupted have ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05F15/00C05F17/20C05F17/80C12R1/07C12R1/685C12R1/645
CPCC12N1/20C12N1/14C05F5/002C05F17/20C05F17/80C05F11/08Y02W30/40
Inventor 郑军荣王应灿罗立津林晨乐占线姚虹余群燕
Owner 福建省固体废物处置有限公司
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