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PH/reduction double-sensitive calcium carbonate-silicon dioxide drug-loaded nano material and preparation method thereof

A silicon dioxide, drug-loaded nanotechnology, applied in the field of nanomedicine, can solve the problems of slow and incomplete drug release at the tumor site, and achieve the effects of controllable reaction speed, improved stability, and cheap and easy-to-obtain raw materials.

Active Publication Date: 2021-11-26
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these technologies have improved the problem of high instability of calcium carbonate, the calcium carbonate nano drug delivery system still has the defect of slow and incomplete drug release at the tumor site. Solving the timely and effective release of drugs at the tumor site is the key to realizing the effective treatment of drugs delivered by nanocarriers. key

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  • PH/reduction double-sensitive calcium carbonate-silicon dioxide drug-loaded nano material and preparation method thereof
  • PH/reduction double-sensitive calcium carbonate-silicon dioxide drug-loaded nano material and preparation method thereof
  • PH/reduction double-sensitive calcium carbonate-silicon dioxide drug-loaded nano material and preparation method thereof

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preparation example Construction

[0035] The preparation method of the present invention comprises the steps:

[0036] Dissolve 150-200 mg of calcium chloride dihydrate and 2-5 mg of dopamine hydrochloride in 80 mL of absolute ethanol, add 3 μL of ammonia solution, and stir well to obtain solution A;

[0037] Dissolve 3-5 mg of antineoplastic drugs in 20 mL of absolute ethanol to obtain solution B; the antineoplastic drugs are those containing carboxyl, hydroxyl or diketone groups, such as: curcumin, doxorubicin, quercetin or resveratrol.

[0038] While stirring, slowly add solution B dropwise to solution A at a rate of 60mL / h, cover the beaker with aluminum foil with uniform holes, and keep solution C under light-shielding conditions, and keep stirring at a speed of 15-20rpm 2-4h, chelating the antineoplastic drug with calcium ions to obtain solution C;

[0039] Put the solution C and 5g of ammonium bicarbonate together in a vacuum drying oven, and react under vacuum conditions with a temperature of 30-40° ...

Embodiment 1

[0044] Add 150mg of calcium chloride dihydrate and 2mg of dopamine hydrochloride into a 250mL beaker, dissolve them with 80mL of absolute ethanol, then add 3μL of ammonia solution dropwise, and stir well to obtain solution A;

[0045] Cover solution A with an aluminum foil filled with even small holes, place it in a vacuum drying oven with 5g of ammonium bicarbonate, and react under vacuum conditions at a temperature of 37°C and a reaction time of 24h to obtain crude calcium carbonate nanomaterials. product. The crude product was centrifuged at 9000 rpm for 7 minutes, washed and centrifuged repeatedly with absolute ethanol for 5 times, and dried at room temperature to obtain calcium carbonate nanocarriers.

[0046] Measure the particle size distribution figure and the degree of dispersion (PDI) of the calcium carbonate nanoparticles obtained with the Malvern laser particle size analyzer, the results can be found in figure 1 In (a) and (b), the hydrated particle size of amorph...

Embodiment 2

[0050] Add 150mg of calcium chloride dihydrate and 2mg of dopamine hydrochloride into a 250mL beaker, dissolve them with 80mL of absolute ethanol, add 3μL of ammonia solution, stir well, and obtain solution A;

[0051] Add 3mg of curcumin into a 100mL beaker, and dissolve it with 20mL of absolute ethanol to obtain solution B;

[0052] While stirring, slowly add solution B to solution A at a rate of 60mL / h to obtain solution C, cover the beaker with aluminum foil with uniform holes, and keep solution C under light-proof conditions at a speed of 15rpm Keep stirring for 2h;

[0053] The solution C and 5 g of ammonium bicarbonate were placed in a vacuum drying oven and reacted under vacuum conditions with a temperature of 37° C. and a reaction time of 24 hours to obtain a crude product of calcium carbonate nanomaterials loaded with antitumor drugs. The crude product was centrifuged at 9000 rpm for 7 minutes, washed and centrifuged repeatedly with absolute ethanol for 5 times, and...

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Abstract

The invention discloses a PH / reduction double-sensitive calcium carbonate-silicon dioxide drug-loaded nano material and a preparation method thereof. The preparation method comprises the following steps: dissolving calcium chloride dihydrate and dopamine hydrochloride in absolute ethyl alcohol, and adding an ammonia water solution to obtain a solution A; dissolving an anti-tumor drug in absolute ethyl alcohol to obtain a solution B; dropwise adding the solution B into the solution A, and stirring under a dark condition to obtain a solution C; reacting the solution C with ammonium bicarbonate at 30-40 DEG C for 6-48 hours to obtain an anti-tumor drug-calcium carbonate nano-carrier; mixing the anti-tumor drug-calcium carbonate carrier, absolute ethyl alcohol, deionized water and an EDTA solution, carrying out ultrasonic treatment, dropwise adding ammonia water, carrying out ultrasonic treatment, and dropwise adding a mixture of tetraethoxysilane and a silane coupling agent containing breakable disulfide bonds; and stirring in a dark place to obtain the pH / reduction double-sensitive calcium carbonate-silicon dioxide drug-loaded nano material. The preparation conditions are mild, the raw materials are simple and easy to obtain, and the purpose of continuously and effectively releasing the medicine at the tumor part can be achieved.

Description

technical field [0001] The invention belongs to the technical field of nanomedicine, and in particular relates to a pH / reduction double-sensitive calcium carbonate-silicon dioxide drug-loaded nanometer material and a preparation method. Background technique [0002] Malignant tumor is a serious fatal disease. The mortality rate has been the highest in my country for many years, and it is gradually increasing, which seriously threatens the health of the people. Chemotherapy is one of the commonly used means to treat malignant tumors. However, due to strong side effects on the human body, low drug utilization and lack of targeting, the treatment of malignant tumors by chemotherapy is greatly limited. In recent years, nanoparticle-based nano-drug delivery systems have been widely used in cancer therapy. There are many differences between tumor cells and normal tissue cells, such as pH value, concentration of redox substances, temperature, and the type and quantity of enzymes. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K47/52A61K9/51A61K47/02A61K45/00A61P35/00B82Y5/00B82Y30/00B82Y40/00C01F11/18
CPCA61K47/6923A61K47/52A61K45/00A61P35/00A61K9/5115B82Y5/00B82Y30/00B82Y40/00C01F11/181Y02A50/30
Inventor 温惠云卫又华徐卓然胡乐乐闫宇斌薛伟明黄赛鹏
Owner NORTHWEST UNIV(CN)