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Development and application of lactobacillus acidophilus NM for inhibiting [alpha]-glucosidase

A Lactobacillus acidophilus and glucosidase technology, applied in the field of probiotic development and application, can solve the problems of low yield, high production cost, poor activity, etc., and achieve the effects of improving yield, resisting colitis and maintaining integrity

Pending Publication Date: 2021-11-30
INNER MONGOLIA PUZE BIOLOGICAL PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a kind of development and application of Lactobacillus acidophilus NM that inhibits α-glucosidase, to solve the activity of the bacterial classification proposed in the above-mentioned background technology is relatively poor, and the yield rate is low during production, making production cost relatively high. high problem

Method used

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  • Development and application of lactobacillus acidophilus NM for inhibiting [alpha]-glucosidase
  • Development and application of lactobacillus acidophilus NM for inhibiting [alpha]-glucosidase
  • Development and application of lactobacillus acidophilus NM for inhibiting [alpha]-glucosidase

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Embodiment 1

[0039] The preparation method of described Lactobacillus acidophilus NM comprises the following steps:

[0040] Step 1: Freeze-dry the seeds, and store the strains in a sterile environment at -40°C;

[0041] Step 2: Seed activation, put the freeze-dried strains stored at -40°C in step 1 in a local class 100 ultra-clean workbench, take the freeze-dried strains and inoculate them in the primary seed medium aseptically, and select the primary seeds for cultivation The colonies in the base were inoculated into the secondary seed medium, and then the colonies in the secondary seed medium were selected to inoculate into the tertiary seed medium. The primary seed medium, the secondary seed medium and the tertiary seed medium were composed The following raw materials are composed in parts by mass: 5 parts of lactose, 2 parts of beef extract, 2 parts of yeast powder, 5 parts of casein peptone, 2 parts of soybean peptone, KH 2 PO 4 2 copies, K 2 HPO 4 2 parts MgSO 4 0.1 part, Tween...

Embodiment 2

[0051] The preparation method of described Lactobacillus acidophilus NM comprises the following steps:

[0052] Step 1: Freeze-dry the seeds, and store the strains in a sterile environment at -40°C;

[0053] Step 2: Seed activation, put the freeze-dried strains stored at -40°C in step 1 in a local class 100 ultra-clean workbench, take the freeze-dried strains and inoculate them in the primary seed medium aseptically, and select the primary seeds for cultivation The colonies in the base were inoculated into the secondary seed medium, and then the colonies in the secondary seed medium were selected to inoculate into the tertiary seed medium. The primary seed medium, the secondary seed medium and the tertiary seed medium were composed The raw materials are composed of the following parts by mass: 8 parts of lactose, 3 parts of beef extract, 3 parts of yeast powder, 7 parts of casein peptone, 3 parts of soybean peptone, KH 2 PO 4 2.5 servings, K 2 HPO 4 2.5 parts, MgSO 4 0.1 ...

Embodiment 3

[0063] The preparation method of described Lactobacillus acidophilus NM comprises the following steps:

[0064] Step 1: Freeze-dry the seeds, and store the strains in a sterile environment at -40°C;

[0065] Step 2: Seed activation, put the freeze-dried strains stored at -40°C in step 1 in a local class 100 ultra-clean workbench, take the freeze-dried strains and inoculate them in the primary seed medium aseptically, and select the primary seeds for cultivation The colonies in the base were inoculated into the secondary seed medium, and then the colonies in the secondary seed medium were selected to inoculate into the tertiary seed medium. The primary seed medium, the secondary seed medium and the tertiary seed medium were composed The following raw materials are composed of the following mass parts: 10 parts of lactose, 5 parts of beef extract, 5 parts of yeast powder, 10 parts of casein peptone, 5 parts of soybean peptone, KH 2 PO 4 2.5 servings, K 2 HPO 4 2.5 parts, MgS...

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Abstract

The invention belongs to the technical field of development and application of lactobacillus acidophilus NM, and particularly relates to development and application of lactobacillus acidophilus NM for inhibiting [alpha]-glucosidase, the development and application comprises the steps of seed freeze-drying, seed activation, fermentation treatment and forming treatment, by means of multiple activation culture of strains, the activity of the strains can be guaranteed, the excellent production performance of the strain is ensured, and meanwhile, the yield is improved; and the lactobacillus acidophilus NM produces a large amount of butyric acid, a small amount of lactic acid, acetic acid and other fatty acids in the fermentation process, the utilization rate of butyric acid is the highest, 75% of oxygen consumption of cultured free colonic epithelial cells comes from butyrate oxidation and mainly participates in gluconeogenesis, ketosome generation, triacylglycerol synthesis and the like, and the traditional Chinese medicine composition indirectly affects metabolism of saccharides and lipids and is the most important energy source of intestinal epithelial cells, so that the traditional Chinese medicine composition has the effects of maintaining integrity of colonic mucosa, resisting colitis, resisting cancers and the like, and is closer to intestinal health.

Description

technical field [0001] The invention relates to the technical field of development and application of probiotics, in particular to the development and application of Lactobacillus acidophilus NM that inhibits α-glucosidase. Background technique [0002] The strains with hypoglycemic effect include-Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus reuteri, Bifidobacterium, indicating that Lactobacillus can inhibit α-glucosidase The activity of probiotics, the mechanism of action of probiotics in alleviating type 2 diabetes --- from the five aspects of intestinal flora, immune response, oxidative stress, short-chain fatty acids and intestinal barrier, we often use α-glucosidase and DPP -IV is a method for screening hypoglycemic strains as the target, and the hypoglycemic effect of probiotics is evaluated through in vivo experiments. Among them, α-glucosidase is located at the brush border ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12N1/02A61K35/747A61P3/10A61P1/00A23L33/135C12R1/23
CPCC12N1/20C12N1/04C12N1/02A61K35/747A61P3/10A61P1/00A23L33/135A23V2002/00A23V2400/113A23V2200/3204A23V2200/328
Inventor 闫继彪孙洁宇李翠平李磊
Owner INNER MONGOLIA PUZE BIOLOGICAL PROD CO LTD
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