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Preparation method of anti-helicobacter pylori egg yolk antibody embedded gel particles

A technology of Helicobacter pylori and egg yolk antibody, applied in the direction of antibodies, antibacterial drugs, chemical instruments and methods, etc., can solve the problems of slow release and destruction of intestinal flora, etc., to slow down the exudation rate, the process is simple, and the operation is simple. handy effect

Pending Publication Date: 2022-01-28
广西康众洋生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This application is aimed at the use of antibiotics to eradicate Helicobacter pylori leading to bacterial drug resistance and side effects, as well as destroying the intestinal flora. At the same time, oral administration of IgY egg yolk antibody causes inactivation and degradation of specific immunoglobulins in gastric acid and pepsin. , a specific immunoglobulin against Helicobacter pylori is extracted and purified from egg yolk by immunizing laying hens with specific antigens, and then the specific immunoglobulin is used for re-aggregation and orifice-coagulation bath method for secondary embedding process Combined to prepare stable gel beads, the gel beads have a high embedding rate, can increase the titer of antibodies, can reduce the activity loss rate of specific immunoglobulins in the stomach, and make igy in the gastrointestinal tract Gel beads contain high activity and have a sustained and slow release effect

Method used

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Embodiment 1

[0052] A preparation method for anti-Helicobacter pylori egg yolk antibody embedding gel particles, comprising the preparation of egg yolk antibody emulsion and two embedding steps, the specific steps are as follows:

[0053] (1) Prepare the water phase: Weigh 10 parts of poloxamer P188, add 900 parts of purified water and heat to 95°C, stop heating, stir to cool to room temperature, add 5 parts of chlorhexidine gluconate, 200 parts of sorbitol, 100 parts of 1 part of sucrose and 50 parts of egg yolk antibody IgY, stirred evenly to form the water phase;

[0054] (2) Preparation of oil phase: Weigh 50 parts and add to 50 parts of ethanol, stir evenly, add 10 parts of povidone k30, 10 parts of Tween-80 and 15 parts of polyoxyl stearate s40, stir evenly to form oil Phase, as microcapsule solidification;

[0055] (3) Egg yolk antibody emulsion: treat the above oil phase into a thin stream, add it into the water phase solution, stir well, add 5 parts of ethylparaben, and stir at a...

Embodiment 2

[0061] A preparation method for anti-Helicobacter pylori egg yolk antibody embedding gel particles, including the preparation of egg yolk antibody emulsion and two embedding steps, the steps are as follows: first neutralize egg yolk antibody IgY with preservatives, acid stabilizers, enzymes Stabilizer, emulsifier, fixative, ethanol, and microcapsule glidant are mixed for emulsification reaction to obtain egg yolk antibody emulsion;

[0062] Then use the combination of complex coacervation method and sharp hole-coagulation bath method for secondary embedding, use gelatin and gum arabic as the wall material, egg yolk antibody emulsion as the core material, then add gum arabic solution and gelatin, and use hydrochloric acid to adjust the pH value , stirring to make the material condense into microcapsules, then mix the microcapsules and sodium alginate solution to form a mixed solution, then slowly drop the mixed solution into the calcium chloride solution, and then wash and dry t...

Embodiment 3

[0072] A preparation method for anti-Helicobacter pylori egg yolk antibody embedding gel particles, including the preparation of egg yolk antibody emulsion and two embedding steps, the steps are as follows: first neutralize egg yolk antibody IgY with preservatives, acid stabilizers, enzymes Stabilizer, emulsifier, fixative, ethanol, and microcapsule glidant are mixed for emulsification reaction to obtain egg yolk antibody emulsion;

[0073] Then use the combination of complex coacervation method and sharp hole-coagulation bath method for secondary embedding, use gelatin and gum arabic as the wall material, egg yolk antibody emulsion as the core material, then add gum arabic solution and gelatin, and use hydrochloric acid to adjust the pH value , stirring to make the material condense into microcapsules, then mix the microcapsules and sodium alginate solution to form a mixed solution, then slowly drop the mixed solution into the calcium chloride solution, and then wash and dry t...

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Abstract

The invention discloses a preparation method of anti-helicobacter pylori egg yolk antibody embedded gel particles. The preparation method comprises the following steps of: mixing an egg yolk antibody IgY with a preservative and the like, and carrying out an emulsion reaction to obtain an egg yolk antibody emulsion; taking gelatin and Arabic gum as a wall material, taking the egg yolk antibody emulsion as a core material, adding the Arabic gum solution and gelatin, adjusting the pH value by using hydrochloric acid, performing stirring to agglomerate the materials into microcapsules, uniformly mixing the microcapsules with a sodium alginate solution to form a mixed solution, slowly dropwise adding the mixed solution into a calcium chloride solution, and performing washing and airing to obtain the anti-helicobacter pylori egg yolk antibody embedded gel particles. The gel bead particles are high in embedding rate, can improve the titer of the antibody and can reduce the activity loss rate of specific immune globulin in the stomach; and the egg yolk antibody Igy gel bead particles in the gastrointestinal tract have high activity, have a continuous slow release effect and eradicate helicobacter pylori in the gastrointestinal tract, so that the treatment effect is achieved, side effects are not generated, and intestinal flora is not damaged.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of anti-helicobacter pylori yolk antibody-embedded gel particles. Background technique [0002] Helicobacter pylori is one of the most common pathogens in the world. It is a bacterium that lives in the stomach. It adheres to the gastric mucosa and intercellular space and can cause diseases such as gastritis, gastric ulcer and gastric cancer. Helicobacter pylori is contagious and has been listed as the first carcinogen of gastric cancer by the World Health Organization (WHO). Human beings are the only source of infection of Helicobacter pylori. To the oral cavity, saliva is the main route of transmission. Only the eradication of Helicobacter pylori is the only way to treat and prevent diseases. Clinically, antibiotic quadruple therapy is used to treat Helicobacter pylori infection, improve the eradication rate of Helicobacter pylori, and prevent dise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/40A61K9/06A61K9/50A61K47/36A61K47/42A61P31/04
CPCA61K9/06A61K9/5057A61K9/5036A61K47/36A61P31/04C07K16/121A61K2039/505
Inventor 农乐根黄炳硕曾正超蒙玉洁
Owner 广西康众洋生物技术有限公司
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