Algae symbiotic bacterium composition, phycomycete co-culture system and microalgae culture method

A technology of symbiotic bacteria and composition, which is applied in the field of algae symbiotic bacteria composition, can solve the problems of decreased dissolved oxygen, little effect of algae inhibition, and consumption of oxygen in water, so as to achieve the effect of increasing production capacity and rapid growth of algae

Pending Publication Date: 2022-03-11
HAINAN GREEN ALGAE WORLD BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And while the algae are multiplying in large numbers, some cells are also aging and dying. The dying cells rupture and degrade, which consumes the oxygen in the water body, causing a sharp drop in dissolved oxygen. In addition, the ruptured cyanobacteria will release cyanotoxins, ammonia nitrogen, and nitrite. Toxic and harmful substances, such as toxic and harmful substances, have brought a series of serious consequences to the aquatic ecosystem
At this time, the growth and reproduction of bacteria in the water body is inhibited, and they are in a dormant state or partially dead. Although alginolytic bacteria and cyanophages can survive, they have little inhibitory effect on algae

Method used

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  • Algae symbiotic bacterium composition, phycomycete co-culture system and microalgae culture method
  • Algae symbiotic bacterium composition, phycomycete co-culture system and microalgae culture method
  • Algae symbiotic bacterium composition, phycomycete co-culture system and microalgae culture method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] 1.1 Medium composition:

[0061] 80g / L NaNO 3 , 15g / L NH 4 HCO 3 , 5g / L NaH 2 PO 4 2H 2 O, 5g / LK 2 HPO 4 ·3H 2 O, 2g / LKH 2 PO 4 , 0.1v / v% Tween 80, 30g / L NaCl, 15g / L yeast extract powder, 30g / L peptone, 10g / L sodium hydroxymethylcellulose, 10g / L sodium diacetate, 100μg / L vitamin and 80mg / L trace elements.

[0062] Microorganisms include vitamin B12, vitamin B1 and biotin in a mass ratio of 1:1:1.

[0063] The trace elements are added in a trace element solution, and the preparation method of the trace element solution is obtained by dissolving in 1L of water according to the formula in the table below.

[0064] Material name level Dosage Na 2 EDTA

AR 2g FeCl 3

AR 0.2g CuSO 4

AR 0.1g ZnSO 4

AR 0.1g CoCl 2

AR 0.05g MnC1 2

AR 0.05g Na 2 MoO 4

AR 0.01g

[0065] Co-culture composition:

[0066]

[0067] 1.2 In a 2L Erlenmeyer flask, prepare 1L of the abov...

Embodiment 2

[0069] 2.1 Medium composition:

[0070] 100g / L NaNO 3 , 14g / L NH 4 HCO 3 , 10g / L NaH 2 PO 4 2H 2 O, 5g / LK 2 HPO 4 ·3H 2O, 1g / LKH 2 PO 4 , 0.1v / v% Tween 80, 30g / L NaCl, 20g / L yeast extract powder, 30g / L peptone, 15g / L sodium hydroxymethylcellulose, 10g / L sodium diacetate, 150μg / L vitamin and 80mg / L trace elements. Wherein the composition of vitamin and trace element is with embodiment 1.

[0071] Co-culture composition:

[0072]

[0073] 2.2 In a 400L vertical photobioreactor, prepare 350L of the above-mentioned medium with filtered seawater, and insert co-cultivation bacteria and seawater chlorella algae (seawater chlorella inoculation density 800,000 / ml), At 32°C, aerated for 96 hours, the light intensity during the cultivation was 6000lux, a mixed gas of carbon dioxide and oxygen was introduced, the ventilation rate was 5vvm (volume ratio per minute), the mixing ratio of carbon dioxide and oxygen was 2:1, and the density of algae was detected daily and vita...

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Abstract

The invention provides an algal symbiotic bacterium composition. The algal symbiotic bacterium composition comprises rhodotorula benthica, a photosynthetic bacterium strain, marine nitrifying bacteria and vibrio. The ratio of the rhodotorula benthica to the photosynthetic bacteria strain to the marine nitrifying bacteria to the vibrio is (20-30) g: (100-200) ml: (10-50) g: (1-3) g; the density of the photosynthetic bacteria strain is 2-5 billion / ml. Compared with the prior art, by means of symbiotic culture of different bacterium combinations and algae, the problems that the microalgae are prone to aging, decaying, breeding of harmful bacteria and the like in the culture process can be solved, the microalgae and beneficial bacteria are subjected to symbiotic culture, then the algae grow faster and more vigorously, and the productivity and yield of the microalgae are improved.

Description

technical field [0001] The invention belongs to the technical field of microalgae cultivation, and in particular relates to an algae symbiotic bacteria composition, an algae-bacteria co-cultivation system and a microalgae cultivation method. Background technique [0002] Seawater chlorella is widely used in the fields of pharmaceutical raw materials, health food and bioenergy because it is rich in protein, lipid, polysaccharide, vitamin, antioxidant and other functional nutrients. At present, seawater chlorella culture technology is mostly concentrated in the photoautotrophic system, which has disadvantages such as low production efficiency, large floor area, and high harvest cost, which seriously restricts the development of seawater chlorella industry. Increasing the biomass of seawater chlorella through artificial large-scale cultivation technology is the key to the development and utilization of seawater chlorella resources. [0003] In recent years, seawater chlorella ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/20C12N1/12C12R1/89C12R1/01C12R1/645
CPCC12N1/16C12N1/20C12N1/12
Inventor 黄成潭潘军叶蕾黄敏
Owner HAINAN GREEN ALGAE WORLD BIOTECHNOLOGY CO LTD
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