Scavernous body injury repair tissue engineering scaffold and preparation method thereof
A tissue engineering scaffold, damage repair technology, applied in tissue regeneration, prosthesis, additive processing, etc., can solve problems such as difficult to solve aesthetics, functionality or ethics, difficult to obtain satisfactory treatment effects, complex cavernous body, etc. , to achieve the effect of repair and functional regeneration, wide applicability and simple preparation
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[0035] The present invention also provides a preparation method of the above-mentioned cavernous body damage repair tissue engineering scaffold, comprising the following preparation steps:
[0036] 1) Dissolving the polymer matrix material in PBS solution or cell culture medium to obtain 3D printing ink;
[0037] 2) Loading the 3D printing ink obtained in step 1) into the 3D printer barrel, printing according to the preset structure, and then cleaning and demoulding to obtain the support base layer after the printing is completed;
[0038] 3) adding bioactive factors and seed cells into the 3D printing ink obtained in step 1) to obtain a bioactive solution;
[0039] 4) The scaffold base layer obtained in step 2) is grafted with the biologically active solution obtained in step 3) by a grafting method to obtain a scaffold base layer with a surface functionalized layer, and the scaffold base layer with a surface functionalized layer is placed in the bioactive solution. After cu...
Embodiment 1
[0047] 1) The grafted double bond of gelatin and hyaluronic acid is modified, and then configured into a relatively viscous aqueous solution, wherein the mass fractions are 10% and 2%, respectively, and photoinitiator I2959 (mass fraction 0.5%) is added to make 3D print ink.
[0048]2) The obtained 3D ink is loaded into a sterile 3D printer barrel, and an orthogonal grid bracket is obtained by extrusion printing. After each layer is printed, ultraviolet light with a wavelength of 365 nm is used for crosslinking for 15 s, and the overall thickness of the obtained bracket base layer is 1 mm. The local filament diameter is 100 μm, and the overall porosity is 30%.
[0049] 3) Immerse the printed scaffold base layer in cell culture medium, coat with polylysine, and use electrostatic adsorption to adsorb exosomes on the surface to make a 10 μm-thick surface functionalized layer, and then inoculate muscle on the surface of the scaffold. Derived stem cells, seeded at a density of 10,...
Embodiment 2
[0051] The difference from Example 1 is that in step 1) of this example, the polymer matrix materials used are sericin and elastin, and the mass fractions are 6.5% and 3%, respectively; The diameter of the silk is 150 μm, and the overall porosity is 40%; in step 3), the bioactive factor used is VEGF protein; the thickness of the obtained surface functionalization layer is 50 μm; the seed cells used are bone marrow mesenchymal stem cells, and the seeding density is 100,000 cells / cm 2 ; The incubation time after inoculation of cells was 48 hours.
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