Self-powered drug-loading-free antibacterial patch

A self-powered, antibacterial patch technology, applied in the direction of local antibacterial agents, antibacterial drugs, sheet-like delivery, etc., can solve problems such as uncontrollable enrichment, and achieve high-efficiency antibacterial and antibacterial effects

Pending Publication Date: 2022-07-12
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of GBFC in the field of drug-free antibacterial patches has not yet been reported. The main difficulty lies in: how to design a new bioanode so that the anode can catalyze the generated H while lowering blood sugar. 2 o 2 Decomposition produces antibacterial agent-ROS; how to design GBFC as a flexible patch; how to regulate H 2 o 2 Uncontrolled enrichment in wounds, preventing its attack on normal tissues and the immune system

Method used

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  • Self-powered drug-loading-free antibacterial patch
  • Self-powered drug-loading-free antibacterial patch
  • Self-powered drug-loading-free antibacterial patch

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] like figure 1 As shown in a, the self-powered drug-free antibacterial patch designed by the present invention includes a support material (medical tape), an anode, a cathode and a resistance device;

[0031] The cathode and the anode are connected by a resistive device, and the support material supports the anode, the cathode and the resistive device;

[0032] The cathode comprises a flexible electrode (carbon cloth) loaded with a functionalized carbon nanotube film, and the functionalized carbon nanotube film is loaded with a cathodic enzyme; the cathodic enzyme is a peroxidase or a peroxidase-like catalyst ;

[0033] The anode comprises a flexible electrode loaded with an anode catalyst, wherein the anode catalyst is MOF encapsulating two biological enzymes with cascade action and carbon nanotubes; the two biological enzymes with cascade action are glucose oxidase and chia Root peroxidase or enzyme-like catalyst of both enzymes.

[0034] The functionalized carbon n...

Embodiment 2

[0036] The construction method of the self-powered drug-free antibacterial patch system designed by the present invention includes the following steps:

[0037] Self-powered drug-free antibacterial patch anode fabrication: First, we encapsulated glucose oxidase (GOD) and horseradish peroxidase (HRP) and single-walled carbon nanotubes (SWCNT) into MAF-7. 0.01-0.06g of polyvinylpyrrolidone was dissolved in 0.5mL of secondary water, 2.00-8.00mg of SWCNT was added, ultrasonically dispersed for 30min, and stirred for 2h. Then, 0.5 mL of a mixed solution containing 100-200 mM 3-methyl-1,2,4-triazole, 4-12 mg of glucose oxidase and 1-6 mg of peroxidase was added and stirred for 30 min. Then add 40 μL of 50-100 mM Zn(NO 3 ) 2 ·6H 2 O, stir for 5min, and finally stand at room temperature for 24h. The obtained enzyme solution was added dropwise to a 5mm*15mm carbon cloth treated by plasma technology, and then placed in a refrigerator at 4°C for drying to obtain a SWCNT-MAF-7-GOx / HRP...

Embodiment 3

[0040] Self-powered drug-free antibacterial patch cathode fabrication: First, single-walled carbon nanotubes / gold nanoparticles (SWCNT / AuNPs) composite powders [for the synthesis method, see Panpan Gai, Rongbin Song, Cheng Zhu, Yusheng Ji, Wengjing Wang , Jian-Rong Zhang and Jun-Jie Zhu, Chemical Communications., 2015, 51, 16763] mixed with 30% polytetrafluoroethylene emulsion in a mass ratio of 80:20, and ground into a cuttable film. It was cut into the desired shape and size, and then press-coated on the flexible electrode-carbon cloth to ensure that the mass of the SWCNT / AuNPs film on the electrode was 10-20 mg cm -2 . The SWCNT / AuNPs composite was used as the substrate material to accelerate electron transfer and provide anchoring sites for HRP. The carbon cloth loaded with SWCNT / AuNPs thin film was mixed with 1 mg mL -1 Incubate the EDC / NHS solution for 30 min to activate the carboxyl groups on AuNPs, and finally add 40 μL of 5-10 mg mL -1 The HRP solution was added dr...

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Abstract

The invention belongs to the technical field of biological energy conversion and medical antibiosis, and particularly relates to a self-powered drug-loading-free antibacterial patch, which is characterized in that glucose oxidase and horse radish peroxidase or enzyme-like catalysts of the two enzymes encapsulated by a metal organic framework material are used as anode catalysts; peroxidase or peroxidase-like is used as a cathode catalyst to form a complete patch which consists of the fuel cell and is free of any drug load; the anode takes glucose molecules in a biological system as fuel to generate electrons and active oxygen free radicals, and the cathode receives the electrons transmitted by the anode and catalyzes decomposition of hydrogen peroxide to generate active oxygen free radicals; the patch can generate an external electric field to promote the electrode to capture bacteria; active oxygen free radicals generated by the electrodes can kill bacteria, so that an antibacterial effect is achieved; starting from three aspects of regulating and controlling a hyperglycemia environment, accurately capturing and sterilizing, and regulating and controlling uncontrollable enrichment of active oxygen, an efficient antibacterial effect is achieved.

Description

technical field [0001] The invention belongs to the technical field of bioenergy conversion and medical antibacterial, in particular to a self-powered drug-free antibacterial patch. Specifically, it involves using glucose oxidase and horseradish peroxidase or an enzyme-like catalyst with cascade catalysis to design a dual-catalyst modified bioanode, using peroxidase or an enzyme-like catalyst as the cathode catalyst; using flexible conductive materials as The base electrode, the constructed patch can improve the high-sugar environment of the wound, generate free radicals while generating electricity, and exert an efficient antibacterial effect, which is expected to be used for wound care of diabetic patients. Background technique [0002] Rapid healing of diabetic wounds is a worldwide problem. Compared with normal wounds, the hyperglycemic environment of diabetic wounds not only delays the process of coagulation and new blood vessel formation, but also acts as a nutrient f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/70A61K33/40A61P3/10A61P17/02A61P31/04A61P31/02
CPCA61K9/7023A61K33/40A61P3/10A61P17/02A61P31/04A61P31/02Y02A50/30
Inventor 王琳琳苏琦雯朱俊杰张剑荣
Owner NANJING UNIV
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