Method for repairing arsenic and antibiotic polluted soil through cooperation of bacteria and magnetic diatomite

A technology for polluted soil and diatomite, applied in the field of soil treatment, can solve the problems of long remediation time, secondary pollution, low remediation efficiency, etc., and achieve the effects of short remediation time, large treatment range and low treatment cost

Pending Publication Date: 2022-07-26
QINGDAO TECHNOLOGICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Heat treatment of arsenic-contaminated soil will also destroy the soil structure, and also face problems such as high treatment costs and operating costs
There are many cases of solidification/stabilization applied to arsenic-contaminated soil, such as cement solidification, chelating agent stabilization, iron-manganese oxide and other inorganic materials, etc., but in order to obtain a bet

Method used

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  • Method for repairing arsenic and antibiotic polluted soil through cooperation of bacteria and magnetic diatomite
  • Method for repairing arsenic and antibiotic polluted soil through cooperation of bacteria and magnetic diatomite
  • Method for repairing arsenic and antibiotic polluted soil through cooperation of bacteria and magnetic diatomite

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Experimental program
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Effect test

Embodiment 1

[0034] A method for restoring arsenic and antibiotic-contaminated soil with bacteria and magnetic diatomite comprises the following steps: bacterial strain isolation → strain enrichment culture → preparation of magnetic diatomite material → soil restoration.

[0035] (1) Isolation, domestication and enrichment of bacterial strains:

[0036] The water sample with the mixed microflora was added to the nutrient broth for shaking culture, and after culturing for 72 hours, the bacterial liquid in the nutrient broth medium was transferred to the PYCM medium (the composition of the medium was: 0.8g / L peptone, 0.2g / L yeast extract powder, 0.1g / L dipotassium hydrogen phosphate, 0.2g / L magnesium sulfate, 0.2g / L sodium nitrate, 0.1g / L calcium chloride, 0.1g / L ammonium chloride, 1g / L Ferric ammonium citrate, 0.2g / L manganese sulfate, 0.1g / L ammonium carbonate, pH=7.0±0.2) to continue the culture.

[0037] Gradual dilution: aspirate the bacterial solution in PYCM and press 10 -1 , 10 -2...

Embodiment 2

[0052] The method for remediating arsenic and antibiotic-contaminated soil by bacteria and magnetic diatomite comprises the following steps: bacterial strain isolation and culture → bacterial strain enrichment culture → preparation of magnetic diatomite material → soil remediation.

[0053] (1) Isolation, domestication and enrichment of bacterial strains:

[0054] The obtained strain was inoculated into nutrient broth (17-25g / L) for enrichment culture;

[0055] Specifically, the strain is Acidophilus Swiftlet WHW-1, and the concentration of the nutrient broth is 19 g / L.

[0056] Further, the conditions for the enrichment culture of the strains are as follows: the temperature is at 25° C. and the frequency is 150 rpm for horizontal shaking, and aerobic enrichment culture is carried out for 5 days. During the enrichment culture, the transfer amount of the strain is 5%.

[0057] (2) Preparation of magnetic diatomite

[0058] By FeCl 3 ·6H 2 O: FeSO 4 ·7H 2 The molar ratio o...

Embodiment 3

[0066] The method for remediating arsenic and antibiotic-contaminated soil by bacteria and magnetic diatomite comprises the following steps: bacterial strain isolation and culture → bacterial strain enrichment culture → preparation of magnetic diatomite material → soil remediation. .

[0067] (1) Enrichment of bacterial species:

[0068] The obtained strain was inoculated into nutrient broth (17-25g / L) for enrichment culture;

[0069] Specifically, the strain is Acidophilus Swiftlet WHW-1, and the concentration of the nutrient broth is 19 g / L.

[0070] Further, the conditions for the enrichment culture of the strains are as follows: the temperature is at 25° C., and the frequency is 150 rpm horizontal shaking, aerobic enrichment culture is carried out for 5 days, and the transfer amount of the strain is 5% by volume.

[0071] (2) Preparation of magnetic diatomite

[0072] By FeCl 3 ·6H 2 O: FeSO 4 ·7H 2 The molar ratio of O is 2:1 to weigh FeCl 3 ·6H 2 O(0.1M) solid, ...

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Abstract

The invention discloses a method for repairing soil polluted by arsenic and antibiotics through cooperation of bacteria and magnetic diatomite. The method comprises the following steps of bacterial strain separation, strain enrichment culture, magnetic diatomite material preparation and soil repairing. The method comprises the following steps: selecting acidovorax agilis WHW-1, and carrying out enrichment culture on the acidovorax agilis WHW-1 in an enrichment culture medium; then, the strain, divalent manganese, a carbon source and the like are added into the composite polluted soil together and stirred uniformly, microorganisms are induced to generate biological manganese oxides, the biological manganese oxides, trivalent arsenic and antibiotics in the soil are subjected to a series of physical-chemical-biochemical reactions, water-soluble arsenic in the soil is converted into a stable state, and the soil is polluted by the microorganisms. The bio-availability of arsenic in the soil is reduced, so that the aim of repairing the arsenic is achieved, and meanwhile, the bacteria and the biological manganese oxide induced by the bacteria convert antibiotics into carbon dioxide and water through intracellular metabolism, oxygenolysis and the like. The process is simple to operate, low in treatment cost and free of secondary pollution.

Description

technical field [0001] The invention relates to the technical field of soil treatment, in particular to a method for restoring soil contaminated by arsenic and antibiotics in cooperation with magnetic diatomite by bacteria. Background technique [0002] Arsenic-contaminated soil remediation technologies mainly include solidification / stabilization, soil leaching, heat treatment, phytoremediation and microbial remediation. Soil leaching is usually the addition of leaching agents (such as organic or inorganic acids, alkalis, salts and chelating agents) to the soil, which will not only destroy the soil microaggregate structure, but also lead to the leaching and precipitation of nutrients. And it is easy to cause secondary pollution problems such as groundwater pollution, which is suitable for soil treatment with small area and heavy pollution. Manganese oxide is a common metal oxidant and adsorbent in soil. Because of its low zero-point charge, high negative charge, large speci...

Claims

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Application Information

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IPC IPC(8): B09C1/10B09C1/08C12N1/36C12N1/02C12N1/20
CPCB09C1/10B09C1/08C12N1/36C12N1/02C12N1/20
Inventor 王华伟张艳茹王亚楠孙英杰王祁赵辛龙段钰而莫泽旭李晓滕家志
Owner QINGDAO TECHNOLOGICAL UNIVERSITY
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