Preparation method of L-arginine-based carbon dots and application of L-arginine-based carbon dots in lemon yellow detection

An arginine-based carbon, tartrazine technology, applied in chemical instruments and methods, nano-carbon, material excitation analysis, etc., can solve the problem of uneven particle size of carbon quantum dots, achieve high selectivity and anti-interference ability, raw materials The effect of low cost, good acid and alkali tolerance and salt tolerance

Pending Publication Date: 2022-08-05
KUNMING UNIV
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  • Abstract
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Problems solved by technology

The synthesis steps of the microwave synthesis method are greatly simplified, but the particle size of the prepared carbon quantum dots is not uniform
[0008] At present, the raw materials for preparing carbon quantum dots are very extensive, including ethanol, citric acid, ethylenediamine, lignin, chitosan, starch, sodium alginate, shiitake mushrooms, cabbage, durian, water hyacinth, ginkgo, aloe, green tea, etc. , but the use of arginine to prepare carbon quantum dots has not been reported

Method used

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  • Preparation method of L-arginine-based carbon dots and application of L-arginine-based carbon dots in lemon yellow detection
  • Preparation method of L-arginine-based carbon dots and application of L-arginine-based carbon dots in lemon yellow detection
  • Preparation method of L-arginine-based carbon dots and application of L-arginine-based carbon dots in lemon yellow detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Preparation of L-Arg-CDs Coarse Carbon Dots

[0049] Arginine-derived CDs were prepared using a one-step hydrothermal method. First, 0.35 g of L-arginine and 0.5 g of o-phenylenediamine were weighed and dissolved in 5 mL of DMF, mixed well, and then transferred to a 20 mL polytetrafluoroethylene high-temperature reaction kettle for reaction at 210 °C for 10 h. After the product was naturally cooled, thin-layer chromatography (TLC) was used to mix the carbon dots with the concentrated solution, and the samples were drawn on a chromatographic silica gel plate. The volume ratio of methanol and dichloromethane was 1:10 as the eluent to separate the carbon dots. The solvent was removed by rotary evaporation to obtain carbon dots with blue fluorescence under ultraviolet light, and then stored at 4 °C until use.

[0050] The solvent (water, DMF, n-propanol, acetone, ethanol and methanol), molar ratio (0.0621, 0.1862, 0.3104, 0.4345, 0.5587), temperature (170℃-220℃)...

Embodiment 2

[0051] Example 2: Structural characterization of L-Arg-CDs coarse carbon dots

[0052] The shape characteristics and particle size distribution of L-Arg-CDs were investigated by transmission electron microscopy (TEM), such as figure 2 As shown, the results indicated that the L-Arg-CDs had regular shapes in the form of spherical-like carbon nanoparticles with an average particle size of 2.75 nm and a lattice spacing of about 0.21 nm. It has good monodispersity and no obvious aggregation, stable structure, can be freely dispersed in water, and has good water solubility.

[0053] The crystal structure of carbon dots is image 3 X-ray powder diffraction (XRD) shown in . The diffraction peak of L-Arg-CDs is 2θ=22.27°, indicating that the synthesized fluorescent carbon quantum dots belong to amorphous carbon structure.

[0054] Using XPS ( Figure 4 ) to analyze the surface functional groups and components of L-Arg-CDs. The characteristic peaks of XPS full spectrum at 284.0eV,...

Embodiment 3

[0059] Example 3: Purification of L-Arg-CDs crude carbon dots

[0060] The quantum yield QY of crude carbon dots was measured after secondary purification, the QY of crude L-Arg-CDs was 14.58%; the QY of secondary purified L-Arg-CDs was 22.670%. QY increased. Measure the fluorescence absorption values ​​of secondary purified L-Arg-CDs and crude L-Arg-CDs, such as Image 6 a It can be seen that the fluorescence performance of the secondary purified L-Arg-CDs is better than that of the crude L-Arg-CDs; 1mME102 was added to both CDs for fluorescence quenching, and the secondary purified L-Arg-CDs was also better than the crude L-Arg-CDs for the quenching of E102 L-Arg-CDs.

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Abstract

The invention relates to a preparation method of L-arginine-based carbon dots and an application of the L-arginine-based carbon dots in lemon yellow detection, which comprises the following steps: dissolving L-arginine and o-phenylenediamine in water, methanol, ethanol, n-propanol, acetone or N, N-dimethylformamide according to a molar ratio of 1: 20-1: 1 at normal temperature; transferring into a polytetrafluoroethylene high-temperature reaction kettle, and reacting at 170-210 DEG C for 2-12 hours; after the product is naturally cooled, centrifuging at a high speed, dialyzing for 24-72 hours by using a 500-5000Da dialysis bag, drying to obtain crude carbon dots, separating and purifying by using column chromatography, carrying out gradient elution by using an eluent, collecting a blue fluorescence part, and recovering the solvent under reduced pressure to obtain relatively pure carbon dots; continuously carrying out secondary gradient elution on the relatively pure carbon dots by using an eluent by adopting the column chromatography, and carrying out thin-layer chromatography detection to obtain fluorescent and ultraviolet dual-mode detection carbon dots; the fluorescence quenching mechanism of the prepared L-Arg-CDs and lemon yellow belongs to an inner filter effect, the optimal linear detection concentration range is 0-55 [mu] M, the detection limit LOD is 42.3 nM and is far lower than the detection limit 187.14 [mu] M provided by the national standard GB/T5009.35-2003, and the L-Arg-CDs and lemon yellow can be used as a rapid detection method of lemon yellow.

Description

technical field [0001] The invention belongs to the technical field of application of fluorescent carbon nanomaterials, relates to a preparation method and application of carbon quantum dots, and in particular relates to a preparation method of L-arginine-based carbon dots and its application in tartrazine detection. technical background [0002] Tartrazine (E102) is one of the most widely used azo synthetic dyes. It is orange-yellow powder, odorless, easily soluble in water, slightly soluble in ethanol, and insoluble in oil. In order to meet people's sensory needs for food, tartrazine is usually used to color the food during the production, processing and transportation of food. However, excessive consumption of tartrazine can seriously harm human health. Studies have shown that excessive intake of tartrazine can cause DNA damage and interfere with DNA synthesis, resulting in fetal malformations; it can even be converted into carcinogens, causing subcutaneous sarcoma, live...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65C01B32/15B82Y20/00B82Y40/00G01N21/64
CPCC09K11/65C01B32/15B82Y20/00B82Y40/00G01N21/643G01N2021/6432C01P2004/04C01P2004/51C01P2004/64C01P2002/72C01P2002/85C01P2002/82C01P2004/32
Inventor 涂渝娇蒋蕾袁琳季秀玲向盈盈宋飞
Owner KUNMING UNIV
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