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Preparation method of hematopoietic tissue repairing material

A tissue organ and tissue repair technology, applied in medical science, prosthesis, etc., to achieve high mechanical strength and toughness

Inactive Publication Date: 2005-01-26
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no material that can meet the needs of small-diameter blood vessels and micro-vessels of complex organs at the same time. Good anticoagulant properties, high mechanical strength and toughness, and a variety of performance tissues and organs that match the degradation rate with the growth rate of blood vessels repair material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1 g of PHBHHx (Mw: 1,000,00 Da) was dissolved in 100 mL of acetone and refluxed at 60° C. for 1 h. The 100,000 molecular weight PHBHHx acetone solution was then centrifuged (5000 g, 20 min) to remove insoluble particles. Methanol was added to the suspension to obtain a high-purity PHBHHx precipitate. The purified PHBHHx and PLGA were dissolved in chloroform at a ratio of 1:10 to prepare a 1% polymer solution. The solution is then poured on a flat plate to make a 3mm thick film-like material;

[0019] Mix 0.1% sodium heparin aqueous solution with acetone at a ratio of 1:1 (V / V). Soak the prepared film-like material in the solution for 4 hours, and directly dry it. The film-like material thus prepared can be used for the repair or repair of the cardiovascular system. It can also be used as an internal stent for vascular anastomosis or vascular stenosis.

Embodiment 2

[0021] Dissolve the purified PHBHHx with a molecular weight of 200,000 and PLGA in chloroform at a ratio of 10:1, stir well and stand still to remove air bubbles. Add NaCI porogen accounting for 100% by weight of the polymer material to the solution prepared above, and then pour it on a polytetrafluoroethylene flat plate to form a thin film.

[0022] Soak the film prepared above in distilled water for 1 day, remove the porogen, and freeze-dry to obtain the tissue repair material.

[0023] Mix 1% dextran aqueous solution with acetone at a ratio of 1:1 (V / V). The film prepared in the above (2) was soaked in the solution for 6 hours, polycondensed in 40%, 30%, 20%, and 10% acetone aqueous solution successively, and then air-dried. The membranes thus produced can be used for repair or repair of the cardiovascular system.

[0024] Composite bone marrow cells in the micropores of the inner and outer layers of the prepared film material, respectively, for better blood vessel repair...

Embodiment 3

[0026] Dissolve the purified PHBHHx and PLLA with a molecular weight of 300,000 in chloroform at a ratio of 10:1 to form a 2% polymer solution, then add paclitaxel with a molar ratio of 1% and 10% NaCl, fully stir and stand still Remove air bubbles. Then it is coated on the periphery of the plastic wire to form a tubular stent.

[0027] 0.1% carboxylated sulfated chitosan was miscible with acetone at a ratio of 1:1 (V / V). The tubular stent prepared above was soaked in the solution for 4 hours, and then air-dried directly or sequentially polycondensed in 40%, 30%, 20%, and 10% acetone aqueous solution and then air-dried. The tubular material thus prepared can be used for repair or repair of the cardiovascular system.

[0028] The tubular material prepared above is respectively compounded with bone marrow cells on the inner and outer layers (or on the inner and outer layers of the tube) and then connected to the damaged blood vessel. It can also be used as an internal stent f...

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Abstract

The invention relates to a preparation process of repairing materials for tissues and organs, in particular to a preparation process of repairing material for tissues and complex organs. It makes PHBHHx as main materials to prepare film type, single tube, multiple tubes and net tube materials. It uses PLLA, PLGA, PCL, PU, other degradable high molecular materials, drug, growth factor, cell and other components to prepare a material for repairing tissues and organs with favorable anticoagulant property, biological compatibility, comparative highly machinery intensity and toughness, and matching degradation rate with the growth rate of the complex organs.

Description

technical field [0001] The invention relates to a biological tissue repair material, in particular to a preparation method of a complex organ repair material. Background technique [0002] There are many kinds of pipeline systems in the human body that often need to be repaired or maintain stable functions due to damage, stenosis and other reasons. Taking blood vessels as an example, blood vessel transplantation is required when the blood vessel is not working properly due to reasons such as arteriosclerosis, aging or damage of blood vessels. Due to the strong rejection of allogeneic organs, the lack of sources, and the high price, the research on artificial blood vessels has attracted more and more attention. As early as the 1940s, some people began to study the transplantation of human blood vessels. At that time, due to limited conditions, hard plastic tubes could be used instead of blood vessels to be implanted into the living body. Due to severe blood coagulation react...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18
Inventor 林峰王小红颜永年熊卓吴任东张人佶卢清萍
Owner TSINGHUA UNIV
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