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KOKUMI-imparting agent

A technology of agent and substance, applied in the field of application No.2006-188458

Active Publication Date: 2008-11-12
AJINOMOTO CO INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Although the development of various kokumi-imparting substances has been attempted as described above, and extracts of natural products have been commercialized mainly, extracts of natural products such as glutathione and N-(4-methyl- 5-oxo-1-imidazolin-2-yl) sarcosine (N-(4-methyl-5-oxo-1-imidazolin-2-yl) sarcosine) is used to isolate pure kokumi components

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0127]

[0128] The gene for the calcium receptor was prepared as follows. Based on the DNA sequence registered at NCBI (calcium receptor: NM_000388), synthetic oligomeric DNAs (forward primer (N) and reverse primer (C)) for PCR were designed (Table 1, SEQ ID NOS: 1 and 2).

[0129] Table 1

[0130] Synthesis of oligo DNAs (forward primer (N) and reverse primer (C), h: human)

[0131] the code

[0132] By using human kidney cDNA (Clontech) as a material, the primers shown in Table 1 (hCASR_N (SEQ ID NO: 1) and hCASR_C (SEQ ID NO: 2)) were synthesized, and by using Pfuultra DNA Polymerase (Stratagene) in the following Perform PCR under conditions. After reacting at 94°C for 3 minutes, a reaction cycle of 94°C for 30 seconds, 55°C for 30 seconds and 72°C for 2 minutes was repeated 35 times, after which the reaction was performed at 72°C for 7 minutes. Whether or not amplification was obtained by PCR was checked by performing agarose electrophoresis, staining with...

Embodiment 2

[0134]

[0135]As L-amino acid samples, 23 kinds of special grade amino acids were used, alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histamine Acid, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, Valine, Ornithine , Taurine (these are all from Ajinomoto) and Hydroxyproline (Nakarai Tesque). D-Cys and D-Typ (Nakarai Tesque) and calcium chloride use special grade. In addition, as peptide samples, γ-Glu-Cys-Gly (Sigma Aldrich Japan), γ-Glu-Cys(SNO)-Gly (Dojin Chemical Laboratory), γ-Glu-Ala (BachemFeinchemikalien AG), γ-Glu- Gly (Bachem Feinchemikalien AG), γ-Glu-Cys (Sigma Aldrich Japan), γ-Glu-Met (Bachem Feinchemikalien AG), γ-Glu-Abu-Gly (Abu: α-aminobutyric acid, Bachem Feinchemikalien AG), γ-Glu-Thr (Kokusan Chemical), γ-Glu-Val (Kokusan Chemical), γ-Glu-Leu (contract manufactured product), γ-Glu-Ile (contract manufactured product), γ-Glu-Orn (Kokusan Chemical), As...

Embodiment 3

[0137]

[0138] Boc-Val-OH (8.69 g, 40.0 mmol) and Gly-OBzl·HCl (8.07 g, 40.0 mmol) were dissolved in dichloromethane (100 ml), and the solution was kept at 0°C. Triethylamine (6.13ml, 44.0mmol), HOBt (1-hydroxybenzotriazole, 6.74g, 44.0mmol) and WSC·HCl (1-ethyl-3-(3-dimethylaminopropyl hydrochloride) Carbodiimide (1-ethyl-3-(3-dimethylaminopropyl)carbodiimidehydrochloride), 8.44 g, 44.0 mmol) was added to the solution, and the mixture was stirred overnight at room temperature. The reaction mixture was concentrated under reduced pressure, and the residue was dissolved in ethyl acetate (200 ml). Wash with water (50ml), 5% citric acid aqueous solution (50ml x twice), saturated brine (brine) (50ml), 5% sodium bicarbonate (sodium hydrogencarbonate) aqueous solution (50ml x twice) and saturated brine (50ml). said solution. The organic layer was dried over anhydrous magnesium sulfate, the magnesium sulfate was removed by filtration, and the filtrate was concentrated under reduc...

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Abstract

A method for screening for a kokumi-imparting substance, which utilizes calcium receptor activity as an index, a kokumi-imparting agent containing a kokumi-imparting substance obtained by the screening method as an active ingredient, a method for producing food or drink such as food, seasoning, and drink imparted with kokumi, and food or drink imparted with kokumi.

Description

technical field [0001] This application claims priority from the following applications: Japanese Patent Application No. 2005-325300 filed on November 9, 2005, Japanese Patent Application No. 2006-188458 filed on July 7, 2006, filed on November 22, 2005 US Provisional Patent Application No. 60 / 738562, and US Provisional Patent Application No. 60 / 807831 filed July 20, 2006, which are incorporated herein by reference. [0002] The present invention relates to a method for screening a kokumi-imparting substance, a kokumi-imparting agent containing a kokumi-imparting substance obtained by the screening method as an active ingredient, for Methods of producing food or drink such as kokumi-imparted food, seasoning and drink, and kokumi-imparted food or drink. Background technique [0003] Calcium receptors, also known as calcium sensing receptors (CaSR), are receptors composed of 1078 amino acids, and are classified in class C seven-transmembrane receptors (G protein-coupled recep...

Claims

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Application Information

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IPC IPC(8): G01N33/566C07K5/00A23L1/48A23L35/00
Inventor 大洲竹晃竹下亘江藤让网野裕右宫村直宏山中智彦长崎浩明
Owner AJINOMOTO CO INC
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