Method for producing spores of clostridium butyricum and application thereof in livestock and poultry rearing

Abstract

The invention discloses a method for producing spores of clostridium butyricum and application thereof in livestock and poultry rearing, and aims to provide a method for simply and conveniently culturing a large amount of spores of the clostridium butyricum with steady activity in order to solve the problems that a current method for culturing the clostridium butyricum has high cost and high equipment requirement or has long production period and low transformation ratio of the spores, and apply a fermentation culture fluid to the rearing process of livestock and poultry to reduce the diarrhea rate of the livestock and poultry and improve the productivity. The method comprises the following steps of: activating the clostridium butyricum, then inoculating the clostridium butyricum to a culture medium which takes soybean cake powder or corn starch or corn steep liquor as a main raw material, and sealing the culture medium for fermentation culture; and diluting an obtained fermented bacterial liquid by tap water or feed to be between 1*105 and 5*105cfu/g, and directly feeding the fermented bacterial liquid to the livestock and poultry. The method has the advantages of simple and convenient process, low cost, high utilization rate of the spores, rich nutrition of the bacterial liquid, effective reduction in the diarrhea rate of the livestock and poultry and the death rate of newborn livestock and poultry, improvement on the productivity, and drug cost reduction.

Description

technical field [0001] The invention relates to a cultivation method of Clostridium butyricum, in particular to a method for producing spores of Clostridium butyricum and its application in preventing poultry and livestock diarrhea. Background technique [0002] Clostridium butyricum, that is, Clostridium butyricum, exists in soil, human and animal intestines, cheese and natural yogurt. It is a Gram-positive anaerobic bacillus that can become a negative bacterium in the later stage of culture. It was first introduced in Japan It is used clinically as an intestinal regulator, and health food such as butyric acid bacteria liquid, powder, and capsule has been developed. It was later used as a veterinary drug to treat intestinal flora disorders in livestock and poultry. Butyric acid bacteria is Clostridium spp., the bacteria are straight or slightly curved, and the spores are round or oval. They are endophytic spores and have strong resistance to the external environment. After...

AI Technical Summary

Problems solved by technology

Although a variety of Clostridium butyricum cultivation methods have been disclosed at present, these methods have the problems of high production cost, high equipment requirements, long production cycle and low spore conversion rate

First, because Clostridium butyricum is an anaerobic bacterium, most production methods need to be carried out in an anaerobic environment maintained by paraffin oil, nitrogen or carbon dioxide, and the equipment is complicated and expensive. Patent 200610125586.X discloses a live Clostridium butyricum The production method of the bacterial preparation, the medium needs to be sterilized by autoclaving and maintained in an anaerobic environment for 45-60 hours by paraffin oil

Patent 200410068185.6 discloses the use of PYG medium to cultivate Lactobacillus acidophilus, Enterococcus faecalis, and Clostridium butyricum, which are mixed to make feed additives for poultry breeding. The medium consumption is large and the cost is expensive

Kuang Qun et al. studied the culture conditions of Clostridium butyricum, using glucose, sucrose, lactose, soluble starch, tryptone, yeast extract, beef extract, NaCl, (NH 4 ) 2 SO 4 、K 2 HPO 4 , MgSO 4 , MnSO 4 , NaHCO 3 , CaCO 3 As a culture medium, there is also the problem of high price, and the cultivation time needs to be as long as 36 hours