Optical probe film and special-purposed organic dye for micro determination of copper ion content in water sample

An optical probe and thin-film technology, applied in organic dyes, organic chemistry, methine/polymethine-based dyes, etc., can solve the problems of cumbersome operation, difficult recycling, and easy introduction of errors, and achieve convenient use, simple production, and low cost. cheap effect

Inactive Publication Date: 2011-10-05
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But what the latter two have in common is that organic dyes are used directly in the solution, and most organic dyes can only be dissolved in organic solvents, which are easy to cause environmental pollution, and are difficult to recycle and cannot be reused; such as the national standard GB 7474-87 water quality copper There are following defects in the determination of sodium diethy

Method used

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  • Optical probe film and special-purposed organic dye for micro determination of copper ion content in water sample
  • Optical probe film and special-purposed organic dye for micro determination of copper ion content in water sample
  • Optical probe film and special-purposed organic dye for micro determination of copper ion content in water sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, synthetic organic dye rhodamine B hydrazide 4-ethoxy carboxyl salicylaldehyde Schiff base

[0034] Add 1.2g of rhodamine B, 50mL of ethanol and 3mL of 85% hydrazine hydrate in a 100mL round bottom flask, heat to 80°C and reflux for 2 hours, filter out the resulting precipitate (rhodamine B hydrazide), and dry it. Dissolve 0.436g of the obtained solid in 20mL of ethanol, add 0.78g of 4-ethoxycarboxysalicylaldehyde, heat to 80°C and reflux in an oil bath for 6 hours, remove the solvent under reduced pressure, wash the solid with ethanol 3 times, and then dry it. That is, the organic dye rhodamine B hydrazide 4-ethoxy carboxyl salicylaldehyde Schiff base (RBHCOOH).

Embodiment 2

[0035] Embodiment 2, the preparation of optical probe film

[0036] 1) The making of chitosan film:

[0037] Take by weighing chitosan (molecular weight 1.9 * 10) of degree of deacetylation 90%. 5 ) 0.1g in a 25ml round bottom flask, add 10ml 2% HAc and stir at room temperature for one day, add 100μl (37%-40%) formaldehyde the next day, and continue stirring for 2 hours. Then pipette 500 μl of it and spread film on one side of the inner wall of the glass cuvette (or on the glass slide), and place the glass cuvette (or glass slide) under the infrared lamp, the exchange of chitosan and formaldehyde solution The joint reaction lasts for 24 hours. After that, cool the glass cuvette (or glass piece) to room temperature, add 0.1mol / L NaOH to soak for 10 minutes (purpose deprotonation), then wash it with a large amount of deionized water, and dry it under an infrared lamp , to obtain a chitosan film with a thickness of 10 μm.

[0038] 2) Fabrication of RBHCOOCl covalently immobili...

Embodiment 3

[0041] Example 3, Quantitative Determination of Trace Copper Ions in Water Samples Using Optical Probe Films

[0042] In Tris-HCl buffer solution (10 mM, pH=7.0) containing 50% acetonitrile, 2 mL of solutions containing different concentrations of copper ions (0-50 μM) were added to a glass cuvette covered with film. Place it at room temperature (25°C) for 3 to 5 minutes, and then measure it at 556nm by a spectrophotometer (see figure 2 ). First determine the working curve (curve equation y=0.026+0.0062x) through a series of standard samples. From the working curve, it can be seen that the method of the present invention has good linearity at least in the range of 2.5-10 μM copper ion concentration. Water samples such as drinking water and river water are analyzed and tested, and finally it is proved that the optical probe film of the present invention can at least realize quantitative analysis of copper ions in the range of 2.5-10 μM in the water samples.

[0043] Simultan...

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Abstract

The invention discloses an optical probe film and a special-purposed organic dye for micro determinations of copper ion contents in water sample. The structural formula of the organic dye provided by the present invention is represented by formula I. On the basis of the organic dye, an active group -COCl is introduced. The -COCl group is subject to an amidation reaction with -NH2 on chitosan, and is fixed on a chitosan film through a covalent bond. With the above processes, an optical probe film is produced. The optical probe film has specific response to copper ions. Because copper ions and organic dye molecules are combined through reversible complexations, when the content of the dissociated copper ions in the water sample is changed, the copper ion content still can be reflected instantly. When in use, the film is immersed in a water sample requiring testing, the film is stood in the water sample for 5 minutes, and analysis and determination can be carried out upon the copper-ion-containing water sample through a spectrophotometer. The optical probe film provided by the present invention has advantages of easy production and low cost. According to the present invention, the optical film does not pollute the water system requiring testing, and can be repeatedly used. With the optical film provided by the present invention, an in situ on-line detection can be realized. (Formula I)

Description

technical field [0001] The invention relates to an optical probe film and a special organic dye used for measuring the content of trace copper ions in water samples. Background technique [0002] Copper ion is a common heavy metal ion that widely exists in the environment. Its presence in the human body in an appropriate amount is beneficial to maintain the normal work of the body, such as participating in hematopoiesis, enzyme reactions, and some redox processes in the body. However, if the metabolic balance of copper ions in the body is disrupted, such as lack or excess of copper ions, it may lead to a series of diseases, such as Menkes syndrome and Wilson syndrome. Therefore, it is very important to detect the content of copper ions in various water qualities related to human life. my country's drinking water standard (GB 5749-85) stipulates that the copper ion content in drinking water shall not exceed 1.0ppm, and the sea water quality standard (GB 3097-1997) requires t...

Claims

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Application Information

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IPC IPC(8): C07D491/107C09B23/04C08L5/08C08J7/12C08J5/18G01N33/52
Inventor 童爱军周昭娟向宇
Owner TSINGHUA UNIV
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