An expression vector containing human insulin gene and its construction method and application

A human insulin and expression vector technology, applied in the preparation methods of insulin and peptides, genetic engineering and other directions, can solve the problems of difficult to expand reproduction, low plant oil content, small seed volume, etc., to improve transformation efficiency, simplify purification process, The effect of increasing the expression
CN102268451AInactive Publication Date: 2011-12-07HEBEI CHEM & PHARMA COLLEGE
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HEBEI CHEM & PHARMA COLLEGE
Publication Date
2011-12-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a human insulin gene-containing expression vector and a construction method and application thereof. The plant expression vector pBINOI disclosed by the invention is obtained by inserting a fusion protein expression box which is driven by a rape oil body protein gene promoter and consists of peanut oil body protein genes and human insulin genes between restriction enzyme cutting sites Hind III and Sac I of a plant double expression vector pBI 121. In addition, the invention provides a method for preparing human insulin by converting oil sunflower with the expression vector to construct a plant bioreactor. By adopting the method, the yield of the human insulin can be improved, and the production cost can be greatly reduced; and the method is suitable for industrial production.
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Description

technical field

[0001] The present invention relates to an expression vector containing human insulin gene and its construction method and application, in particular to an expression vector containing human insulin gene and its construction method and a method for preparing human insulin in sunflower oil using the vector. Background technique

[0002] In recent years, the incidence of diabetes has increased year by year, presenting an epidemic trend. Diabetes has become a common and frequently-occurring disease in many countries in the world. Recent figures from the World Health Organization point out that about 3.2 million people worldwide die from diabetes every year, that is, 6 people every minute.

[0003] In diabetes medication, insulin is one of the most effective therapeutic drugs, and it is also the only therapeutic drug for patients with type I diabetes. In addition, 30% to 40% of patients with type II diabetes will eventually need to use insulin. At present, the...

Claims

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