Gradient active scaffold material for osteochondral repair and its preparation method and application

A scaffold material, osteochondral technology, applied in medical science, prosthesis and other directions, can solve the problems of poor repair ability, low metabolic rate physiological characteristics, disease transmission and other problems, and achieve the effect of simple preparation method, firm adhesion and adhesion promotion

Inactive Publication Date: 2011-12-14
SANITARY EQUIP INST ACAD OF MILITARY MEDICAL SCI PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, adult articular cartilage lacks direct blood supply and innervation, and has the physiological characteristics of low metabolic rate, and its self-repair ability is extremely poor. Cartilage defects with a diameter greater than 2 mm can hardly be completely repaired. If combined with subchondral bone defects, it is more difficult to treat
There are obvious disadvantages in the methods for the treatment of osteochondral injuries in clinical application: (1) Oral medicine, intra-articular injection of hyaluronic acid, joint lavage and debridement, etc., can only relieve symptoms and delay the progress of the disease , unable to repair damaged cartilage and subchondral bone
(2) Drilling, microfracture and other operations can use a small amount of MSCs that naturally infiltrate into the drilling area through the medullary cavity without special treatment to repair the defect, but the new stimulation is scar tissue and type I collagen. The main fibrocartilag

Method used

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  • Gradient active scaffold material for osteochondral repair and its preparation method and application
  • Gradient active scaffold material for osteochondral repair and its preparation method and application

Examples

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Example Embodiment

[0051] Example 1

[0052] (1) Using type I collagen, calcium nitrate tetrahydrate, and diammonium hydrogen phosphate as raw materials, the pH of ammonia is adjusted to 8.0, and HA / Col powder is prepared by the co-precipitation method. In the prepared HA / Col-I composite powder, Type I collagen content is 35%, hydroxyapatite content is 65%;

[0053] (2) Use the EDC / NHS-Heparin method to construct a biologically active substance controlled release system, and soak the HA / Col-I composite powder and the pre-prepared type II collagen powder in 0.05mol / LMES buffer (pH 5.6) 30min standby, dissolve each 2.5g heparin in 300ml 0.05mol / L MES buffer containing 2gEDC and 1.5gNHS for 10min, and put the spare collagen into the MES containing heparin and EDC / NHS according to the weight ratio of collagen to heparin 1:2 In the buffer, gently shake the reaction at 37°C for 4 hours; then place the modified HA / Col-I composite powder and type II collagen powder in a 70ng / ml BMP PBS solution (containing ...

Example Embodiment

[0056] Example 2

[0057] (1) Using type I collagen, calcium nitrate tetrahydrate, and diammonium hydrogen phosphate as raw materials, adjusting the pH value of ammonia to 8.0, preparing HA / Col powder by co-precipitation method, in the prepared HA / Col-I composite powder , The type I collagen content is 20%, and the hydroxyapatite content is 80%;

[0058] (2) Use the EDC / NHS-Heparin method to construct a biologically active substance controlled release system, and soak the HA / Col-I composite powder and the pre-prepared type II collagen powder in 0.05mol / LMES buffer (pH 5.6) 30min standby, dissolve each 3g heparin in 200ml 0.05mol / L MES buffer containing 1gEDC and 2gNHS for 10min, and put the spare collagen into the MES buffer containing heparin and EDC / NHS according to the weight ratio of collagen to heparin 1:3 In the medium, gently shake the reaction at 37°C for 4h; then put the modified HA / Col-I composite powder and type II collagen powder in a PBS solution (containing 0.5% BSA,...

Example Embodiment

[0061] Example 3

[0062] (1) Using type I collagen, calcium nitrate tetrahydrate, and diammonium hydrogen phosphate as raw materials, adjusting the pH value of ammonia to 8.0, preparing HA / Col powder by co-precipitation method, in the prepared HA / Col-I composite powder , The content of type I collagen is 50%, and the content of hydroxyapatite is 50%;

[0063] (2) Use the EDC / NHS-Heparin method to construct a biologically active substance controlled release system, and soak the HA / Col-I composite powder and the pre-prepared type II collagen powder in 0.05mol / LMES buffer (pH 5.6) 30min standby, dissolve each 1g heparin in 500ml 0.05mol / L MES buffer containing 3gEDC and 0.5gNHS for 10min activation, according to the weight ratio of collagen to heparin 1:1, put the spare collagen into the MES buffer containing heparin and EDC / NHS In the solution, gently shake the reaction at 37°C for 4h; then place the modified HA / Col-I composite powder and type II collagen powder in a PBS solution w...

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Abstract

The invention relates to a gradient active scaffold material for osteochondral repair and its preparation method and application. The active scaffold material is mainly composed of a cartilage layer, a calcified layer and a subchondral bone layer respectively loaded with biologically active substances, wherein the calcified layer is placed Between the cartilage layer and the subchondral bone layer, between the cartilage layer and the calcified layer, and between the calcified layer and the subchondral bone layer are organically connected by a biomedical degradable polymer; the cartilage layer, the calcified layer and the subchondral bone layer It has interpenetrating pores; its preparation method is: (1) preparation of HA/Col-I composite powder; (2) construction of bioactive substance controlled release system; (3) molding preparation of gradient active scaffold material for osteochondral repair The active scaffold material is used to prepare osteochondral complex; the active scaffold material can effectively promote the adhesion, proliferation and differentiation of cells, and is suitable for the repair and reconstruction of bone and cartilage and the delivery of nutrients.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a gradient active scaffold material for osteochondral repair and its preparation method and application. Background technique [0002] Osteochondral defects in joints are very common in clinical practice, often leading to joint pain, limited mobility, osteoarthritis, and even limb dysfunction and disability, which seriously affect the quality of life of patients and have become one of the main causes of physical disabilities. one. However, adult articular cartilage lacks direct blood supply and innervation, has the physiological characteristics of low metabolic rate, and has extremely poor self-repair ability. Cartilage defects with a diameter > 2 mm can hardly be completely repaired. If combined with subchondral bone defects, it is more difficult to treat. There are obvious disadvantages in the methods for the treatment of osteochondral injuries in clinical application:...

Claims

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Application Information

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IPC IPC(8): A61L27/40C08J9/26
Inventor 李志宏武继民郭涛张西正关静李瑞欣郭勇黄姝杰侍才洪马军
Owner SANITARY EQUIP INST ACAD OF MILITARY MEDICAL SCI PLA
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