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Reagent for glucose content measurement by enzyme electrode method

A technology of glucose and enzyme electrode, which is applied in the field of reagents for detecting glucose content by enzyme electrode method, can solve the problems of influence and interference in the detection of hemolyzed samples, and achieve the effects of good correlation, fast detection speed and reliable results.

Active Publication Date: 2012-10-17
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The glucose enzymatic colorimetric method is based on the enzymatic reaction of glucose under the catalysis of GOD or HK to produce substances with characteristic absorption peaks, and the rate of absorbance change is measured at a specific wavelength to calculate the concentration of glucose in the solution, but the traditional enzyme Colorimetry is susceptible to interference from endogenous substances in the sample, such as vitamin C, uric acid, urea, bilirubin, creatinine, and hemolyzed samples will also affect its detection

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  • Reagent for glucose content measurement by enzyme electrode method
  • Reagent for glucose content measurement by enzyme electrode method
  • Reagent for glucose content measurement by enzyme electrode method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] PH6.0 citric acid-trisodium citrate buffer solution 200mmol / L

[0033] GOD: 300KU / L

[0034] Absolute ethanol: 5%

[0035] Potassium iodide: 50mmol / L

[0036] Ammonium molybdate: 50mmol / L

[0037] Glycerol: 5g / L

[0038] Sodium azide: 5g / L.

[0039] Test samples, the GLUm module on the Beckman LX20 automatic biochemical analyzer, use the above reagents, that is, the glucose enzyme electrode method reagent for sample testing, and at the same time use the glucose enzyme colorimetric method reagent (HK) on the CC part of the biochemical analyzer method) for sample testing.

[0040] 40 clinical serum samples were tested with the reagent of the present invention and the HK method reagent respectively. The former completed all the tests in approximately 15 minutes and produced the results, while the latter completed the tests in approximately 40 minutes and produced the results. The test results showed that the serum glucose values ​​were roughly distributed in the rang...

Embodiment 2

[0042] PH6.0 Tris buffer 50mmol / L

[0043] GOD: 50KU / L

[0044] Absolute ethanol: 0.1%

[0045] Sodium iodide: 2mmol / L

[0046] Ammonium molybdate: 2mmol / L

[0047] Octanol: 0.5gL

[0048] Formaldehyde: 0.5gL.

[0049] For the test sample, the GLUm module on the Beckman LX20 automatic biochemical analyzer uses the above reagents, that is, the glucose enzyme electrode method reagent for sample testing, and at the same time, the CC part of the biochemical analyzer uses the glucose HK method reagent for sample testing.

[0050]Respectively use the reagent of the present invention and the HK method reagent to detect the samples with high linear value according to gradient dilution, the results show that the detection linearity of the former can reach 50mmol / L, while that of the latter can reach 35mmol / L.

Embodiment 3

[0052] PH6.0 acetic acid-sodium acetate buffer solution 500mmol / L

[0053] GOD: 500KU / L

[0054] Absolute ethanol: 10%

[0055] Potassium iodide: 100mmol / L

[0056] Ammonium molybdate: 100mmol / L

[0057] Glycerol: 10g / L

[0058] PC300: 10g / L

[0059] For the test sample, the GLUm module on the Beckman LX20 automatic biochemical analyzer uses the above reagents, that is, the glucose enzyme electrode method reagent for sample testing, and at the same time, the CC part of the biochemical analyzer uses the glucose HK method reagent for sample testing.

[0060] Respectively use the reagent of the present invention and the HK method reagent to detect the serum samples added with interfering substances, the results show that the former reaches 30g / L for unconjugated bilirubin, 28.8g / L for conjugated bilirubin, 3g / L for vitamin C, and 3g / L for hemoglobin. The turbidity of chyle reaches 500g / L, and the turbidity of chyle reaches 1450 without interference; while the latter respecti...

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Abstract

The invention discloses a reagent for glucose content measurement by enzyme electrode method. The reagent contains buffer, glucose oxidase, anhydrous ethanol, iodized salt, ammonium molybdate, stabilizer and preservative. The reagent for glucose content measurement provided by the invention utilizes enzyme electrode for measurement, and is suitable for an automatic biochemical analyzer equipped with a glucose enzyme electrode. Compared with a traditional glucose enzyme colorimetric method, the method of the invention has advantages of good correlation, good anti-interference performance, fast detection and wide linear range; and in particular, the reagent can meet the need of emergent clinical biochemistry.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to a reagent for detecting glucose content by an enzyme electrode method. Background technique [0002] Determination of glucose is a clinically important detection item, because glucose content is an important physiological indicator of the human body, it is widely used clinically, and is in great demand. Glucose in the body mainly comes from food, which is the transportation form of sugar in the body. Through the mutual regulation and balance between glycogen generation and glycogen decomposition, glycogeneogenesis and glycogen fermentation, fat formation and lipolysis, blood sugar The relative constant of the protein is of great significance to maintain the normal physiological functions of the body. At present, the clinical manifestations of high or low glucose content are: 1. Elevated glucose: the most common hyperglycemia is diabetes; pancreatitis, pancre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 邹炳德邹继华张桂春
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD