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Application method of plant-derived fungicide in plant disease prevention and control

A plant-derived fungicide, a technology for preventing and controlling plant diseases, applied in the directions of botanical equipment and methods, fungicides, plant growth regulators, etc., to achieve the effects of strong bacteriostatic effect, simple production process and low production cost

Inactive Publication Date: 2012-11-21
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wood wax tree belongs to the folk traditional medicinal plants in my country. Its roots, leaves, bark, and fruits have the effects of promoting blood circulation and relieving pain, removing blood stasis, reducing swelling, and treating scabies. The records of rice pests, however, the research on the inhibition of pathogenic bacteria by the chemical components of wax tree at home and abroad is still blank, and there is no report on the prevention and treatment of plant bacterial diseases and oomycete diseases by methyl 3,4,5-trihydroxybenzoate
The present invention is based on the separation and purification of the antibacterial active components of the leaves of the wax tree, and uses its antibacterial compounds to develop a botanical fungicide, which is the first case at home and abroad

Method used

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  • Application method of plant-derived fungicide in plant disease prevention and control
  • Application method of plant-derived fungicide in plant disease prevention and control
  • Application method of plant-derived fungicide in plant disease prevention and control

Examples

Experimental program
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Effect test

Embodiment 1

[0014] Embodiment 1: the extraction of the antibacterial substance of wax tree leaf

[0015] Dry the collected leaves of the wax tree in the shade, then dry them at 50°C for 24-48 hours, crush them with a 40-mesh sieve grinder, and reflux them with methanol for 3 times, each time for 3 hours. The combined extracts were concentrated under reduced pressure in a water bath at 50°C with a rotary evaporator to obtain methanol extracts from the leaves of Sarcophagus spp., and the filtered plant residues were dried in the sun for later use. The methanol extract was extracted with ether, and the extract was concentrated to obtain an ether extract.

Embodiment 2

[0016] Example 2: Separation and purification of 3,4,5-trihydroxybenzoic acid methyl ester in the ether extract of the leaves of the wax tree

[0017] The ether extract of the leaves of the wax tree was mixed with reagent-type silica gel (100-200 mesh), and the silica gel (100-200 mesh) column chromatography was used to mix the sample with petroleum ether (60-90 ° C) / acetone (5:1 and 1:1). 1) For gradient elution of the eluent, combine the components of petroleum ether / acetone (1:1), mix the sample with silica gel (100-200 mesh), use silica gel (100-200 mesh) column chromatography, and use chloroform / methanol (10:1) was used as the eluent for elution, and the obtained components were mixed with silica gel (100-200 mesh), and silica gel (200-300 mesh) column chromatography was used, and chloroform / methanol (20:1 ) as the eluent, and finally the obtained components were mixed with silica gel (200-300 mesh), and silica gel (200-300 mesh) was used for column chromatography, and p...

Embodiment 3

[0018] Example 3: Toxicity of methyl 3,4,5-trihydroxybenzoate to Larsia solanacearum

[0019] The tested bacterial species, Ralstonia solanacearum, was provided by the Plant Pathology Research Office of Guangxi University, which belonged to race 1 biochemical type III, and was stored in sterilized water at 20°C. The test bacteria were first streaked and activated on NA solid medium (Nutrient agar, formula: yeast powder 3g, peptone 5g, glucose 10g, agar 17g, water 1000mL, pH 7.0-7.2) for 24-48h, and then transferred to NA culture Suspension culture (28°C, 120rpm) in liquid for 24h. The toxicity of methyl 3,4,5-trihydroxybenzoate to Larsia solanacearum was determined by plate dilution method. Dissolve methyl 3,4,5-trihydroxybenzoate with 10% dimethyl sulfoxide, add different concentrations of drug solution into NA medium, and prepare a series of drug-containing liquid plates. Dilute the bacterial suspension to 10 3 CFU / mL, take 100 μL and smear it on the plate, take the plate...

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Abstract

The present invention relates to an application method of a plant natural compound in plant disease prevention and control, and specifically to an application method of 3,4,5-methyl trihydroxybenzoate in toxicodenddron sylvestre leaves in prevention and control of plant bacterial diseases and oomycete diseases, wherein the 3,4,5-methyl trihydroxybenzoate can be artificially synthesized, and further can be separated and purified from a plurality of plants. According to the present invention, ether is adopted to extract antibacterial substances in toxicodenddron sylvestre leaf dry powder, a silica gel column chromatography method is adopted to separate and purify the 3,4,5-methyl trihydroxybenzoate, a production cost is low, and a production process is simple. The 3,4,5-methyl trihydroxybenzoate has a wide bacterial inhibition spectrum, and plant diseases can be prevented and controlled by applying toxicodenddron sylvestre plant residues or a 3,4,5-methyl trihydroxybenzoate agent. With the present invention, a new resource is provided for creation of new pesticides and research and development of bionic pesticides, and the toxicodenddron sylvestre plant resource is completely developed and utilized.

Description

technical field [0001] The invention relates to an application method of plant natural compounds in the prevention and treatment of plant diseases, in particular to an application method of methyl 3,4,5-trihydroxybenzoate in the prevention and treatment of plant bacterial diseases and oomycete diseases. The invention belongs to the field of biological control of plant diseases. Background technique [0002] The advent of organic synthetic chemical pesticides plays an important role in controlling crop pests and ensuring agricultural production and income. However, while the large-scale use of chemical pesticides produces huge social and economic benefits, it also brings 3 "R" problems such as residue, resistance and resurgence of diseases and insect pests. In order to improve the human living environment and food quality, countries all over the world are conducting research on reducing the use of chemical pesticides. The development direction of pesticides has shifted from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N37/40A01P1/00A01P3/00A01G13/00
Inventor 黎起秦袁高庆林纬秦健
Owner GUANGXI UNIV
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