Anti-ageing wrinkle reduction toning lotion enriched with active peptide, and preparation method thereof

[0060] The preparation method of Angelica extract: Crush the Angelica sinensis, defatted with petroleum ether, and air dry. Add to distilled water at the ratio of sample mass (g): volume of distilled water (ml) of extraction solvent (ml) = 1:10, and let stand for 30 min to moisturize Wet the material, irradiate the microwave 450W for 1min, and then irradiate it for 1min after an interval of 1min, and irradiate at this interval until the total time of microwave extraction reaches 5min. The extraction is carried out by 70W ultrasonic vibration for 40min, the extraction temperature is 75℃, and the filter is filtered to obtain filtrate and filter residue. Add the filter residue to distilled water and repeat the extraction once under the same conditions. Combine the filtrate, add 3g activated carbon, stir for 15min to decolorize, the filtrate obtained after filtration is vacuum concentrated to a paste with a rotary evaporator, and then freeze-dried with a freeze dryer at a low temperature at 0°C to make a dry powder to obtain an angelica extract.

[0061] The preparation method of bamboo leaf extract: except that Angelica sinensis is replaced with bamboo leaf, the rest of the operation is the same as the preparation method of Angelica sinensis extract to obtain the bamboo leaf extract.

[0062] The preparation method of Ganoderma lucidum extract: Except that Angelica sinensis is replaced by Ganoderma lucidum, the rest of the operation is the same as the preparation method of Angelica sinensis extract to obtain the Ganoderma lucidum extract.

[0063] Fish scale collagen peptide was purchased from Hubei Ruibang Biotechnology Co., Ltd., with a number average molecular weight less than 1000 Daltons.

[0064] The preparation method of soft-shelled turtle active polypeptide was prepared by Yin Jinlian's preparation method of soft-shelled turtle active polypeptide described in "Study on the Extraction, Isolation and Functional Characteristics of Soft-shelled Turtle Polypeptide" (Yin Jinlian. Study on the extraction and separation of soft-shelled turtle polypeptide and its functional characteristics. Master's degree thesis: Northwest Agriculture and Forestry University of Science and Technology, 2007, 6). The turtle active polypeptides obtained by the preparation method described in this article are all suitable for the present invention. The following method for preparing turtle active polypeptides includes: removing fat from turtles and blanching them (95℃, 10~ 15s) Remove the skin film to obtain the soft-shelled turtle bones, add water that accounts for 10 times the quality of the soft-shelled soft-shelled turtle bones, cook the soft-shelled soft-shelled turtle bones and take the soup, add water to adjust the soup to 10 times the original quality of the soft-shelled turtle bones, and inoculate Bacillus subtilis was enzymatically hydrolyzed at 37°C and 120r/min shake flask for 72h, heated to 90°C for 15min to inactivate the enzyme, centrifuged, filtered, filtrate dialysis, and gel chromatography were separated to obtain the active polypeptide of turtle.

[0065] Other raw materials are cosmetic-grade commercial products.

[0066] Example 1

[0067] The anti-aging and wrinkle-removing lotion rich in active peptides is composed of active components and additives. The additives are an appropriate amount of G-2 preservative (diazaalkylimidazolium) and an appropriate amount of tea tree essential oil. The active ingredients are composed of the following mass percentages Composition:

[0068]

[0069] Steps:

[0070] (1) Heat hyaluronic acid, turtle active peptides and fish scale collagen peptides in an appropriate amount of deionized water to 55°C to fully dissolve them, and stir evenly to prepare an aqueous phase.

[0071] (2) Mix the luba oil, L-vitamin C, D-panthenol, water-soluble vitamin E, citric acid, taurine, hydroxyethyl urea, angelica extract, bamboo leaf extract and Ganoderma lucidum extract under stirring slowly Add the water phase and homogenize for a few minutes. When cooled to 40°C, add tea tree essential oil and G-2 preservative (diazoalkylimidazolium) and stir evenly. Let stand to room temperature to obtain anti-aging rich in active peptides. Wrinkle lotion finished product.

[0072] Example 2:

[0073] The anti-aging and wrinkle-removing lotion rich in active peptides is composed of active components and additives. The additives are an appropriate amount of G-2 preservative (diazaalkylimidazolium) and an appropriate amount of tea tree essential oil. The active ingredients are composed of the following mass percentages Composition:

[0074]

[0075] The operation steps are the same as in Example 1.