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Method for rapidly collecting algae by magnetic flocculation nanoparticles and application of method

A nanoparticle and flocculation technology, applied in chemical instruments and methods, single-cell algae, water/sludge/sewage treatment, etc., can solve the problems of difficult harvesting, high energy consumption, and difficult filtration, and achieve a wide range of applications. , strong operability, fast effect

Active Publication Date: 2015-05-20
广东牧海生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although it is of great economic significance to use microalgae to produce diesel oil, it is difficult to harvest because microalgae cells form a stable dispersion system in the culture medium, so the separation of microalgae and culture medium has become an important link in the production of biodiesel
[0003] At present, the commonly used harvesting methods are as follows: 1) collection by sedimentation method, which uses the gravity of algae cells for natural settlement and collection. This method has low cost but low efficiency; 2) collection by centrifugation and bubble loading method. High energy consumption and high cost; 3) Filtration method collection, due to the small size of microalgae, general filter paper is difficult to filter, and ultrafiltration method will block the filter holes due to the deposition of algae liquid, and the filtration is difficult to continue; 4) Hydrophobic method is to use the principle of algal hydrophobicity and interaction to collect algal cells, but this method is only applicable to algae liquid in high salt concentration, and the effect on algae liquid cultivated in fresh water is poor, so it is not universal; 5) Ultrasound The method consumes a lot of energy, and the ultrasonic frequency has no significant effect on the harvesting effect; 6) The electrophoresis method has power loss in the process of voltage transformation and rectification, so the existing electrophoresis method consumes a lot of energy for harvesting microalgae, and it is difficult to really apply it. Large-scale industrial harvesting; 7) The flocculation method takes a long time to collect, and the collected algae mud is relatively loose and takes up a large volume; 8) The magnetic adsorption method can quickly collect the algae, but the existing magnetic adsorption method The time spent on collecting algae and the scope of application cannot meet the needs of use

Method used

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  • Method for rapidly collecting algae by magnetic flocculation nanoparticles and application of method
  • Method for rapidly collecting algae by magnetic flocculation nanoparticles and application of method
  • Method for rapidly collecting algae by magnetic flocculation nanoparticles and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Recovery of Chlorella pyrenoidosa:

[0060] (1) Preparation of magnetic flocculation nanoparticles: weigh 0.5g Fe 3 o 4 Nanoparticles (20nm, Aladdin Company, the same below), disperse it into 20ml acetic acid solution containing chitosan (the concentration of acetic acid solution is 1% by volume, and the final concentration of chitosan with a molecular weight of 200,000 is 3mg / ml), adjust the pH value to 4.0 with 0.1mol / L hydrochloric acid, ultrasonicate at 30kHz for 30min at room temperature, and then stir at a speed of 300r / min. During the stirring process, add 3ml of sodium tripolyphosphate solution with a concentration of 2mg / ml dropwise (pH value is 7.0), the dropping rate is 30 drops (one drop is equivalent to 50 microliters) / min, and kept stirring for 30 minutes, wherein the mass ratio of iron ferric oxide nanoparticles, chitosan and sodium tripolyphosphate is 5:0.6 : 0.06, the magnetic flocculation nanoparticle suspension was obtained after the reaction was c...

Embodiment 2

[0071] Recovery of Scenedesmus obliquus:

[0072] (1) The preparation of magnetic flocculation nanoparticles and the method of determining the biomass of microalgae in the microalgae culture solution are the same as in Example 1.

[0073] (2) Determination of the concentration of Scenedesmus obliquus, such as figure 2 As shown, the OD value has a good linear relationship with the concentration of Scenedesmus obliquus.

[0074] (3) Harvesting Scenedesmus obliques by magnetic flocculation nanoparticles

[0075] ①Harvesting at low biomass

[0076] Scenedesmus obliquus [algae species provided by algae species room of Jinan University Red Tide and Marine Biology Research Center (already in the literature "tert-butyl p-hydroxyanisole and norfloxacin on aquatic organisms. Ecological Science, 2007 ( 01)" public), using the improved BG11 medium, at 25°C, the light intensity is 1500μmol·m -2 ·s -1 , light condition 12L:12D incubator culture] the initial period of culture (biomass ...

Embodiment 3

[0082] (1) Determination of harvesting effect at low biomass:

[0083] At the initial stage of two kinds of microalgae cultivation (the culture solution containing chlorella is the same as embodiment 1, the biomass of microalgae is 3×10 7 cell / ml, the culture solution containing Scenedesmus is the same as in Example 2, and the biomass of microalgae is 3×10 7 cell / ml) for harvesting, take 1L of algae liquid (pH is 6.5) in a graduated cylinder, and then add 0, 0.123, 0.246, 0.492, 0.738 and 0.984g of magnetic flocculation nanoparticles prepared in Example 1 to the vector cylinder respectively, Do three repetitions, mix and stir evenly, observe the formation of polymers, and immediately place them on an electromagnet (magnetic field strength 0.5T) for harvesting.

[0084] The measurement results show that the natural sedimentation recovery rate of microalgae is 8.02% when no microparticles are added, and the recovery rate of microalgae is greatly improved after adding microparti...

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Abstract

The invention discloses a method for rapidly collecting algae by magnetic flocculation nanoparticles and application of the method. The method comprises the steps of: firstly, adjusting the pH value of microalgae culture solution to be more than or equal to 6.0, adding the magnetic flocculation nanoparticles into the microalgae culture solution, mixing and stirring to obtain polymer of microalgae cells and the magnetic flocculation nanoparticles; then, carrying out magnetic adsorption on the polymer of the microalgae cells and the magnetic flocculation nanoparticles by a magnetic field generator; and finally, separating to obtain the polymer of the microalgae cells and the magnetic flocculation nanoparticles. The method can be used for collecting the algae within 4minutes, and is wide in pH application scope, less in dosage of the magnetic flocculation nanoparticles and low in cost; and the method is high in site operability, the components of microalgae are not damaged by the whole process, and the whole process is free from pollution. Thus, the method provided by the invention not only can be used for collecting the algae on a large scale, and but also is used for sewage treatment.

Description

technical field [0001] The invention relates to a method for collecting algae bodies, in particular to a method and application for rapidly collecting algae bodies by using magnetic flocculation nanoparticles. Background technique [0002] Today, as the impact of the energy crisis on various industries is increasing, all walks of life are paying more and more attention to renewable energy. As an alternative fuel to fossil energy, biodiesel has become the fastest growing and most widely used environmentally friendly renewable energy in the world. . Traditional biodiesel raw materials have been concentrated in fields such as aged grains and lignin, and their production has led to a large demand for crops, resulting in a shortage of crops. The oil content in energy algae cells is high, up to about 80%, which has the potential to convert high-quality fuel oil, and is a type of economical new bioenergy. Although the use of microalgae to produce diesel oil has important economic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C02F3/32
Inventor 江天久温众杰李宏业
Owner 广东牧海生物技术有限公司