Cultural method of iron sheet dendrobe protocorm rich in selenium and chromium

Abstract

The invention discloses a cultural method of iron sheet dendrobe protocorm rich in selenium and chromium. The method comprises the steps of: carrying out induction of the protocorm and enrichment culture of the protocorm by using iron sheet dendrobe seed, stem tip or root tip as explant, wherein the enrichment culture medium components are as follows: every 1L of 1 / 2MS culture medium contains 100 mL of coconut milk, 0.2 mg of NAA, 1.0 mg of KT, 30 g of cane sugar, 0.3 mg of sodium selenite, 0.35 mg of chromium trichloride and 8 g of agar; pH is 5.6-5.8. The embryo-germinated protocorm, which is subjected to induction culture for 30-40 days and is as large as a sesame grain, is transferred to the enrichment culture medium, each bottle contains about ten grains of protocorm; and the protocorm is cultured under conditions that the temperature is 25 DEG C, the illumination intensity is 1500-2000 lux and the illumination time is 1-16 h / d. The culture method disclosed by the invention has the beneficial effects that 1, the whole process is performed at room temperature without being limited by region, season and weather, and is free of pollution; 2, the culture process is simple, easy to operate, short in period and low in subsequent investment; and 3, the iron sheet dendrobe protocorm has high multiplication capacity, high yield and high microelement enrichment ratio.

Description

technical field [0001] The invention relates to a method for cultivating protocorms of dendrobium officinale, in particular to a method for cultivating protocorms of dendrobium officinale rich in selenium and chromium. Background technique [0002] Selenium is an essential trace element for human body and animals. It is closely related to the health of the body. It is a strong antioxidant that can reduce toxic peroxides to harmless substances. It can decompose hydrogen peroxide and is harmful to cells and tissues. Protective effect, so that it is not damaged by peroxides, and has a special protective effect on cell membranes and organelles (mitochondria, microsomes, lysosomes) membranes. As early as the early 1950s, chromium was believed to play a role in glucose metabolism, and was called "glucose tolerance factor" at that time. It has been found from in vitro and animal experiments that chromium can improve insulin resistance and promote the maximum biological effect of i...

AI Technical Summary

Problems solved by technology

Dendrobium candidum itself has the characteristics of high difficulty in cultivation and slow growth, but selenium and chromium elements have some positive and negative effects on its growth, so there are few studies at present, especially the research on selenium-rich and chromium-rich protocorms of Dendrobium candidum. There are currently no reports