Leucine aminopeptidase detection reagent
A technology of leucine aminopeptidase and detection reagent, which is applied in the field of medical inspection, can solve the problems of lack of inspection professionals, insufficient automatic biochemical inspection equipment and facilities, and achieves the effects of good sensitivity, low price and easy operation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0036] Example 1
[0037] Diluent:
[0038] Tris(hydroxymethyl)amino buffer (pH7.0) 0.1mol / L
[0039] TWEEN80 5%
[0040] Proclin300 0.1%
[0041] Bilirubin Oxidase 1KU / L
[0042] Vitamin C oxidase 1KU / L
[0043] Reagent:
[0044] Tris(hydroxymethyl)amino buffer (pH6.0) 0.1mol / L
[0045] Sodium chloride 1mmol / L
[0046] L-leucine-4-nitroaniline 20mmol / L
[0047] Potassium sorbate 0.1g / L
[0048] Bovine serum albumin 5g / L
Example Embodiment
[0049] Example 2
[0050] Diluent:
[0051] Tris(hydroxymethyl)amino buffer (pH7.5) 0.5mol / L
[0052] TRITON X-100 5%
[0053] Methyl p-hydroxybenzoate 1%
[0054] Potassium ferrocyanide 1mol / L
[0055] Vitamin C oxidase 2KU / L
[0056] Reagent:
[0057] 3-(N-morpholine) ethanesulfonate buffer solution (pH7.2) 0.5mol / L
[0058] Sodium chloride 5mmol / L
[0059] L-leucine-4-nitroaniline 50mmol / L
[0060] Proclin300 1g / L
[0061] Trehalose 10g / L
Example Embodiment
[0062] Example 3
[0063] Diluent:
[0064] 3-morpholinopropanesulfonic acid buffer (pH6.5) 1.0mol / L
[0065] SPAN20 10%
[0066] Sodium benzoate 1%
[0067] Potassium ferricyanide 10mol / L
[0068] Vitamin C oxidase 10KU / L
[0069] Reagent:
[0070] Piperazine-N,N-bis(2-hydroxyethanesulfonic acid) buffer (pH8.0) 1mol / L
[0071] Sodium chloride 20mmol / L
[0072] L-leucine-4-nitroaniline 100mmol / L
[0073] Proclin300 1g / L
[0074] Fatty alcohol polyoxyethylene ether 10g / L
[0075] The performance of the reagent obtained in Example 1 of the present invention will be described below in conjunction with the table.
[0076] 1. Precision
[0077] Table 1. Precision evaluation results
[0078]
[0079] 2. Linear
[0080] Different concentrations of analytes are added to the standard serum samples for detection, and the linearity results of LAP are shown in figure 1 .
[0081] 3. Methodological comparison test
[0082] Compared with the LAP result value measured on the automatic biochemical analyzer, the resu...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap