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Method for preparing PLGA (polylactic-co-glycolic acid) modified biological factor microsphere supported bone substituted material

A biological factor and bone replacement technology, applied in the field of medical biomaterial preparation, can solve the problems of no cells, affecting the adhesion and growth of osteoblasts and vascular endothelial cells, and limited applications

Active Publication Date: 2014-07-23
PEKING UNIV SCHOOL OF STOMATOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the inert surface of aliphatic polyester materials has no specific position information for cells to identify, and has disadvantages such as insufficient hydrophilicity and cell affinity, which affects the adhesion of osteoblasts and vascular endothelial cells on its surface. growth, becoming one of the main obstacles restricting its use as an ideal tissue engineering scaffold material
The microstructure of nano-hydroxyapatite (nHA) and β-tricalcium phosphate is similar to natural bone matrix, and has a similar size to the inorganic minerals in natural bone, which helps human cells and macromolecules to recognize it, thereby improving the quality of materials. Bioactivity, availability and biocompatibility; at the same time, the calcium and phosphorus ions released by nHA can participate in bone metabolism and can better promote bone formation, but its application is limited due to its low strength and high brittleness

Method used

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  • Method for preparing PLGA (polylactic-co-glycolic acid) modified biological factor microsphere supported bone substituted material
  • Method for preparing PLGA (polylactic-co-glycolic acid) modified biological factor microsphere supported bone substituted material
  • Method for preparing PLGA (polylactic-co-glycolic acid) modified biological factor microsphere supported bone substituted material

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Experimental program
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Effect test

Embodiment 1

[0026] The preparation method of the modified PLGA biofactor-loaded microsphere bone substitute material comprises the following steps:

[0027] Preparation of drug-loaded chitosan microspheres: 900 mg of carboxymethyl chitosan was dissolved in 29 ml of 2% acetic acid solution by volume to form the first solution; 500 μg of human recombinant adiponectin and 500 μg of human recombinant bone morphogenetic The protein (rhBMP-2 in this example, Genscript Pharmaceuticals, Korea) was dissolved in 1 ml of acetic acid solution with a volume fraction of 2% to form a second solution; the first solution and the second solution were dissolved in 300 ml containing 2 wt% Span- 80 in liquid paraffin, stirred evenly at room temperature to form an emulsion; slowly drop 70mL TPP solution with a mass fraction of 5% into the above emulsion, and fully complete the crosslinking by stirring; The cross-linked product was repeatedly washed and freeze-dried to obtain drug-loaded chitosan microspheres. ...

Embodiment 2

[0031] The preparation method of the modified PLGA biofactor-loaded microsphere bone substitute material comprises the following steps:

[0032]Preparation of drug-loaded chitosan microspheres: 1500 mg carboxymethyl chitosan was dissolved in 40 ml of 2% acetic acid solution by volume to form the first solution; 1 mg of human recombinant adiponectin and 1 mg of human recombinant bone morphogenetic The protein (rhBMP-2 in this example, Genscript Pharmaceuticals, Korea) was dissolved in 2ml of acetic acid solution with a volume fraction of 2% to form a second solution; the first solution and the second solution were dissolved in 500ml containing 2wt% Span- 80 in liquid paraffin, stirred evenly at room temperature to form an emulsion; slowly drop the solution containing 5 mg of sodium triphosphate into the above emulsion, and fully complete the crosslinking by stirring; repeatedly wash with petroleum ether, isopropanol and double distilled water in sequence The cross-linked produc...

Embodiment 3

[0036] The preparation method of the modified PLGA biofactor-loaded microsphere bone substitute material comprises the following steps:

[0037] Preparation of drug-loaded chitosan microspheres: 2000 mg carboxymethyl chitosan was dissolved in 50 ml of 2% acetic acid solution by volume to form the first solution; 2 mg of human recombinant adiponectin and 2 mg of human recombinant bone morphogenetic The protein (rhBMP-2 in this example, Genscript Pharmaceuticals, Korea) was dissolved in 5ml of acetic acid solution with a volume fraction of 2% to form a second solution; the first solution and the second solution were dissolved in 600ml containing 2wt% Span- 80 in liquid paraffin, stirred at room temperature to form an emulsion; slowly drop the solution containing 8 mg of sodium triphosphate into the above emulsion, and fully complete the cross-linking by stirring; repeatedly wash with petroleum ether, isopropanol and double distilled water The cross-linked product can be freeze-d...

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Abstract

The invention relates to a method for preparing a PLGA (polylactic-co-glycolic acid) modified biological factor microsphere supported bone substituted material. The method comprises the following steps: 1, preparing a drug-loaded chitosan microsphere from carboxymethyl chitosan, recombinant human adiponectin, recombinant human bone morphogenetic protein and sodium tripolyphosphate; 2, modifying the metaphysis, serving as a bone raw material, of a newborn calf articular head or tibia with a modifying agent, calcining the modified bone raw material at 800-1100 DEG C to completely remove the immunizing antigenicity to obtain modified calcined bone powder; and 3, preparing the PLGA modified biological factor microsphere supported bone substituted material from PLGA, the modified calcined bone powder and the drug-loaded chitosan microsphere. The PLGA modified biological factor microsphere supported bone substituted material has certain morphological and mechanical strength, and can be used for sustainably releasing biological active factors for promoting bone formation and locally releasing calcium and phosphor ions on a bone defect position.

Description

technical field [0001] The invention relates to the technical field of preparation of medical biomaterials, in particular to a preparation method of a biofactor-loaded and slow-release bone defect repair material with high activity, which can be applied to the repair of bone defects in the body. Background technique [0002] Adiponectin (APN) is a hormone secreted by animal and human adipocytes. It consists of 247 amino acids and has two receptors (AdipoR1 and AdipoR2). Both osteoblasts and osteoclasts express adiponectin receptors. body. In vitro experiments have shown that adiponectin activates the AdipoRl / JNK and AdipoRl / p38 signaling pathways to induce the proliferation and differentiation of human osteoblasts and promote their osteogenic function. At the same time, adiponectin treatment of mouse monocytes can inhibit the activation of NF-kappa B pathway, thereby inhibiting the differentiation and maturation of mouse monocytes into osteoclasts. Animal studies in vivo a...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/54A61L27/36A61L27/50A61L27/12A61L27/22
Inventor 唐志辉毋育伟李箐
Owner PEKING UNIV SCHOOL OF STOMATOLOGY
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