Relative quantification method based on novel mass discrepancy labels for carboxylic acid type signal molecules in biological system

Abstract

The invention provides a quantification method based on novel mass discrepancy labels for relative concentrations of carboxylic acid type signal molecules in a biological sample system, and provides a mass discrepancy label molecule pair labeled by a light / heavy isotope pair and a synthetic method thereof. The mass discrepancy label molecule pair comprises a light isotope label molecule and a heavy isotope label molecule. The formula of the two label molecules is shown as the formula (I). The light / heavy heavy isotope pair exists in one or two of a structure part A and a structure part B in the structure, wherein the light / heavy heavy isotope pair in the structure part A is selected from one or more of a group comprising H / D, 12C / 13C and 14N / 15N, or the light / heavy heavy isotope pair in the structure part B is one or more selected from a group comprising H / D, 12C / 13C and 16O / 18O.

Description

Technical field [0001] The invention provides a method for quantifying the relative content of carboxylic acid signal molecules in a biological sample system based on a novel mass difference label molecule. The method is a high-sensitivity and high-throughput relative quantitative method. The invention also provides a quality difference label molecule pair marked by a light / heavy isotope pair and a synthesis method thereof. Background technique [0002] Carboxylic acid signaling molecules are certain carboxyl-containing chemical molecules that naturally exist in organisms. Their role is to transmit information between and within cells, and participate in many processes of regulating biological growth and development. The research results have great application value. In order to study the mechanism of carboxylic acid signal molecules in depth, it is necessary to study the changes in their content. However, the quantitative analysis of carboxylic acid signal molecules mainly face...

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Problems solved by technology

However, this method mainly has the following two defects: some signal molecules cannot be detected or the sensitivity is poor; stable isotope-labeled signal molecule standards need to be used as internal standards, some are difficult to obtain, and the only standard species are rare and expensive

However, the target of this method is only one type of signaling molecule, which cannot satisfy the simultaneous relative quantitative analysis and interaction network research of multiple types of signaling molecules; in addition, the error of this in vitro labeling method is relatively large, because the comparison samples have been subjected to multiple treatments before combined analysis. Separate extraction, derivatization labeling and solid-phase extraction processing, the errors of these processes cannot be compensated, and the cost, time and effort of the analysis are increased by passing through the solid-phase extraction column alone, and the sample throughput is low [Huang YQ, et al.(2011 )Use ofisotopemass probes for metabolic analysis of the j asmonate biosynthetic pathway. Analyst 136:1515-1522]

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