Relative quantification method based on novel mass discrepancy labels for carboxylic acid type signal molecules in biological system

A mass difference, signal molecule technology, applied in the preparation of amino compounds, chemical instruments and methods, material separation, etc., can solve problems such as difficulty in obtaining, low sample throughput, and inability to detect signal molecules, saving manpower and improving samples. The effect of flux

Inactive Publication Date: 2014-07-23
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method mainly has the following two defects: some signal molecules cannot be detected or the sensitivity is poor; stable isotope-labeled signal molecule standards need to be used as internal standards, some are difficult to obtain, and the only standard species are rare and expensive
However, the target of this method is only one type of signaling molecule, which cannot satisfy the simultaneous relative quantitative analysis and interaction network research of multiple types of signaling molecules; in addition, the error of this in vitro ...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Relative quantification method based on novel mass discrepancy labels for carboxylic acid type signal molecules in biological system
  • Relative quantification method based on novel mass discrepancy labels for carboxylic acid type signal molecules in biological system
  • Relative quantification method based on novel mass discrepancy labels for carboxylic acid type signal molecules in biological system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Synthesis of (2-bromoethyl)trimethylammonium bromide (BETA) 9-deuterated isotope labelled molecule D 9 -BETA

[0110] 1.12g D 9 -Trimethylamine reacts with 7.1mL 1,2-dibromoethane in a dry ice-acetone bath under the action of 6mL dry methanol;

[0111] After the reaction mixture returned to room temperature, it was stirred at room temperature for 2 days;

[0112] The formed white solid was filtered and washed with cold ether to obtain 2.64g of product D. 9 -BETA, 62% yield.

[0113] use 1 H-NMR ( 1 H-NMR), high resolution mass spectrometry (HR-MS) and high resolution tandem mass spectrometry (HR-MS / MS) analysis confirmed the synthesis of D 9 -BETA. Take TMS as internal standard, D 2 O is the solvent, 1 H-NMR (300Hz, Bruker ARX300NMR spectrometer) analysis results are as follows: 3.737(s, 4H), Figure 4 . The mass spectrometer is equipped with an electrospray ion source (ESI) (Waters Synapt TM G2 high resolution mass spectrometry), monitored in positive ion mode, the analysis ...

Embodiment 2

[0115] Relative quantitative analysis of the above 8 carboxylic acid signal molecules in 0.8 mg dry weight (DW) model plant Arabidopsis (Col-0) treated with methyl jasmonate (MeJA) and untreated for 12 days

[0116] Treatment of samples in the treatment group: Add 4 mL of 100 mM MeJA (solvent of 0.1% ethanol) to a culture dish of Arabidopsis whole seedlings grown under normal conditions for 10 days, and then put them in the greenhouse for 2 days after 10 minutes. Grind down and freeze-dry

[0117] Control sample treatment: Add 4 mL of 0.1% ethanol (blank solvent) to the culture dish of Arabidopsis thaliana whole seedlings grown for 10 days under normal conditions, and then put it in the greenhouse for 2 days after 10 minutes, and then under liquid nitrogen after sampling Grind and freeze-dry

[0118] Weigh 0.8mg dry weight (DW) samples of the control group and the treatment group, extract them with methanol and blow dry at room temperature, re-dissolve them in 150μL of acetonitrile,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a quantification method based on novel mass discrepancy labels for relative concentrations of carboxylic acid type signal molecules in a biological sample system, and provides a mass discrepancy label molecule pair labeled by a light/heavy isotope pair and a synthetic method thereof. The mass discrepancy label molecule pair comprises a light isotope label molecule and a heavy isotope label molecule. The formula of the two label molecules is shown as the formula (I). The light/heavy heavy isotope pair exists in one or two of a structure part A and a structure part B in the structure, wherein the light/heavy heavy isotope pair in the structure part A is selected from one or more of a group comprising H/D, 12C/13C and 14N/15N, or the light/heavy heavy isotope pair in the structure part B is one or more selected from a group comprising H/D, 12C/13C and 16O/18O.

Description

Technical field [0001] The invention provides a method for quantifying the relative content of carboxylic acid signal molecules in a biological sample system based on a novel mass difference label molecule. The method is a high-sensitivity and high-throughput relative quantitative method. The invention also provides a quality difference label molecule pair marked by a light / heavy isotope pair and a synthesis method thereof. Background technique [0002] Carboxylic acid signaling molecules are certain carboxyl-containing chemical molecules that naturally exist in organisms. Their role is to transmit information between and within cells, and participate in many processes of regulating biological growth and development. The research results have great application value. In order to study the mechanism of carboxylic acid signal molecules in depth, it is necessary to study the changes in their content. However, the quantitative analysis of carboxylic acid signal molecules mainly face...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07C211/63C07C209/12G01N30/02
Inventor 闫存玉孙晓红欧阳玥褚金芳杨军
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap