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Baphicacanthus root polysaccharide and selenized modifier thereof as well as preparation method and application of selenized modifier

A technology of Nanbanlangen and polysaccharides, which is applied in the field of medicinal chemistry, can solve the problems of low activity and insufficient research data of Nanbanlangen

Inactive Publication Date: 2014-10-01
GUANGXI TEACHERS EDUCATION UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Southern Radix Isatidis is the dry root of the Acanthaceae plant, Isatidis, which has lower free amino acid content than Radix Radix Radix, and its activity in antipyretic, anti-inflammatory, sterilizing viruses and bacteria is lower than that of Radix Radix Radix Radix Radix Radix Radix Radix, so the field of medicine has been used for many years. There is still little research data on Radix isatidis. In recent years, although many reports have tried to use Southern Radix Radix as medicine, they all made it into a compound preparation so that it has pharmacological activities similar to Radix Radix.

Method used

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  • Baphicacanthus root polysaccharide and selenized modifier thereof as well as preparation method and application of selenized modifier
  • Baphicacanthus root polysaccharide and selenized modifier thereof as well as preparation method and application of selenized modifier
  • Baphicacanthus root polysaccharide and selenized modifier thereof as well as preparation method and application of selenized modifier

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Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1: Extraction, separation and purification of Nanlan Radix polysaccharide

[0030] 1.1 Extraction of Nanlan Radix Polysaccharide

[0031] After washing and pulverizing the root of Radix isatidis, weigh 50.000g of dry powder in a Soxhlet extractor, reflux with petroleum ether for 3 hours, reflux with ethanol for 4 hours, and dry in the air. g / mL), the extraction time is 2h, extract three times with water, filter, combine the extracts, concentrate under reduced pressure to 50mL, add four times of 95% ethanol, leave it in the refrigerator overnight, filter with suction, and use anhydrous Wash with ethanol and acetone three times, and dry in an oven at 60°C to obtain crude polysaccharides.

[0032] 1.2 Deproteinization and decolorization of crude polysaccharides

[0033] Weigh 5.000g of crude polysaccharide in a beaker, add 200mL of water to dissolve, add 3% (w / v) papain, adjust the pH to 6.5, stir at 39°C for 48h, add Savage reagent with 1 / 5 of the volume of p...

Embodiment 2

[0039] Embodiment 2: the compositional analysis of Nanlan Radix polysaccharide

[0040] 2.1 Firstly, the purity of the polysaccharide was analyzed by gel column chromatography

[0041] Weigh 0.010 g each of NBLG1 and NBLG2, dissolve them in 4 mL of distilled water, pass through a Sephadex G-75 dextran gel column, elute with 0.1 mol / L sodium chloride aqueous solution, and detect with the phenol-concentrated sulfuric acid method. refer to Figure 2a , 2b As shown in the elution curve, the elution curve of polysaccharide NBLG1 has a single symmetrical peak, which means that it is a pure polysaccharide with a uniform component; the elution curve of polysaccharide NBLG2 has multiple peaks, which shows that it is a mixed polysaccharide with multiple components.

[0042] 2.2 Then use the specific rotation method to repeatedly verify the purity of the polysaccharide

[0043] Each weighed 0.030g of NBLG1 and NBLG2, prepared two portions of 15mg / mL polysaccharide solution with distil...

Embodiment 3

[0057] Embodiment 3. Synthesis of Radix Radix Radix Selenium Polysaccharide

[0058] 3.1 Selenides can be purchased from the market as needed, or can be quickly prepared by the following method

[0059] Weigh 0.400g of selenium powder in a two-necked flask, add 10mL of distilled water, pass through nitrogen protection, stir gently at room temperature, slowly add sodium borohydride aqueous solution (0.390g of sodium borohydride is dissolved in 3.5mL of distilled water), and wait for the selenium powder After the completely dissolved solution becomes clear, add chloroacetic acid or 3-chloropropionic acid aqueous solution (dissolve 0.950g chloroacetic acid or 1.100g 3-chloropropionic acid in 10mL distilled water, adjust the pH of the solution to 9 with saturated sodium carbonate), and continue to stir the reaction 24h. After the reaction was completed, filter twice, adjust the pH of the filtrate to 3 with 10% dilute hydrochloric acid, then extract with ethyl acetate, take the up...

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Abstract

The invention discloses baphicacanthus root polysaccharide and a selenized modifier thereof as well as a preparation method and application of the selenized modifier. The disclosed baphicacanthus root polysaccharide is a heteropolysaccharide and consists of mannose, rhamnose, xylose, arabinose and glucose, wherein the ratio of structural units of the five monosaccharides is 2:3:5:6:1. The disclosed selenized modifier refers to baphicacanthus root selenium polysaccharide and is prepared by reacting the baphicacanthus root polysaccharide, organic acid selenide and sulfoxide chloride. The baphicacanthus root selenium polysaccharide prepared based on the baphicacanthus root polysaccharide has an effect of obviously inhibiting multiple tumor cell strains and can be used for preparing medicines for treating cancers.

Description

technical field [0001] The invention relates to the selenium polysaccharide of Radix isatidis with tumor-inhibiting activity, and belongs to the field of medicinal chemistry. Background technique [0002] The common isatidis, i.e. northern isatidis, is the dry root of the cruciferous plant Isatis indigo, because northern isatidis has good inhibitory effects on a variety of pathogens, including staphylococcus, bacillus, streptococcus, and diplococcus. Influenza virus, Japanese encephalitis virus, mumps virus, influenza virus and other fields have been widely used, especially antipyretic and anti-inflammatory effects are significantly better than Nanbanlangen. [0003] Southern Radix Isatidis is the dry root of the Acanthaceae plant, Isatidis, which has lower free amino acid content than Radix Radix Radix, and its activity in antipyretic, anti-inflammatory, sterilizing viruses and bacteria is lower than that of Radix Radix Radix Radix Radix Radix Radix Radix, so the field of m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00
Inventor 盛家荣李锦金黄珊肖琦邹修文黄初升杨啟源
Owner GUANGXI TEACHERS EDUCATION UNIV
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