Serum-free culture system for efficiently culturing human umbilical cord mesenchymal stem cells in vitro

Abstract

The invention relates to a serum-free culture system for efficiently culturing human umbilical cord mesenchymal stem cells in vitro. The serum-free culture system comprises the following components: a basal culture medium a-MEM, cell factors, hormones and proteins, unsaturated fatty acids, antioxidants, energy substances, an amino acid additive, vitamins and metal additives. The serum-free culture system comprises simple and clear components, is free from harms of pathogens, does not have difference between batches, has good repeatability, can obtain plenty of high-quality human umbilical cord mesenchymal stem cells in a short time and has high passage stability, so that the serum-free culture system can be applied to scientific researches and can provide high-purity vibrant cells for cell therapy as a mating system in cell therapy.

Description

technical field [0001] The invention relates to a biological culture medium, in particular to an efficient serum-free culture system for culturing human umbilical cord mesenchymal stem cells in vitro. Background technique [0002] In recent years, animal cell culture technology has expanded from the field of simple experimental operation to research and commercial utilization. Many biotechnology research and products depend on cell culture. In the history of cell culture development, the development of culture medium occupies an important place. Mesenchymal stem cells are adult stem cells derived from mesoderm with high self-renewal ability and multi-lineage differentiation potential, which widely exist in connective tissue and organ interstitium throughout the body. A large number of experiments have proved that long-term cultured mesenchymal stem cells have a wide range of differentiation potentials, and the terminal tissues formed include brain, retina, liver, intestine...

AI Technical Summary

Problems solved by technology

Serum is essential in the in vitro culture system of cells. Although it can provide growth factors, binding proteins, hormones, and play a certain protective role, it has the following defects: ① The composition is complex and contains some substances that are not conducive to cell growth factors or toxic substances, which may affect the research results; ② There may also be pathogens, which pose a threat to clinical application; ③ There are differences between different batches, resulting in differences in culture results

However, on the one hand, the above-mentioned medium has more components, and the amino acids include L-arginine, L-cystine, L-histidine, L-isoleucine, L-leucine, L-lysine , L-methionine, L-phenylalanine, L-threonine, L-tryptophan, L-tyrosine, L-valine, L-alanine, L-aspartic acid Acid, L-Asparagine, L-Glutamic Acid, Glycine, L-Proline, L-Serine; Vitamins Contains Calcium Pantothenate, Choline Chloride, Folic Acid, Meso-Inositol, Niacinamide, Pyridoxine Hydrochloride Aldehydes, riboflavin, thiamine hydrochloride; lipids containing arachidonic acid, cholesterol, DL-alpha-tocopheryl acetate, linoleic acid, linolenic acid, myristic acid, oleic acid, palmitoleic acid, palmitic acid , stearic acid; trace elements include Cu, Zn, Se, Fe, Sn, Ni, Ag, Al, Cr, Ge, Zr, Rb, Co, Cd, Ba, K, the composition is too complex, causing inconvenience in preparation; On the other hand, the medium should be able to ensure the stability of cell subculture to meet the needs of scientific research. The stability of the above-mentioned medium subculture is not yet clear.

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