Use of origanum vulgare oil in preparation of diquat toxicity-resistant drugs and origanum vulgare oil oral emulsion

A technology for anti-dysentery oil and uses, applied in the direction of anti-virus, drug combination, and resistance to vector-borne diseases, etc.

Inactive Publication Date: 2015-04-01
GUANGZHOU MERITECH BIO ENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And a large number of animal experiments also show that: antibacterial, growth-promoting, feed utilization-enhancing effects, etc., is also a medicinal feed additive of botanical origin approved for use in my country, but the toxic reaction caused by using it to resist diquat is particularly It is the related report of gastrointestinal injury that has not been seen yet

Method used

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  • Use of origanum vulgare oil in preparation of diquat toxicity-resistant drugs and origanum vulgare oil oral emulsion
  • Use of origanum vulgare oil in preparation of diquat toxicity-resistant drugs and origanum vulgare oil oral emulsion
  • Use of origanum vulgare oil in preparation of diquat toxicity-resistant drugs and origanum vulgare oil oral emulsion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The establishment of embodiment 1 Diquat (Diquat) injury model

[0028] 1 Test method

[0029] 1.2 Animal grouping and feeding methods

[0030] Twelve 7-8-week-old female Wistar rats of clean grade were selected and randomly divided into a control group and a diquat-induced oxidative stress treatment group (hereinafter referred to as the treatment group). There were 6 replicates in each group, with 1 rat per replicate, housed in a single cage in the same room, with natural light, free access to food and water. The basic diet formula of Wistar rats is shown in Table 1.

[0031] Table 1 Basic diet of experimental mice

[0032] Components

g / kg

corn

570g

soybean meal

180g

wheat bran

170g

fish meal

50g

egg yolk powder

10g

skimmed milk powder

15g

salt

5g

vitamin premix a

1g

Trace element premix b

1.6g

[0033] a Addition per kilogram of diet: 7500IU...

Embodiment 2

[0086] Example 2 The preventive and therapeutic effect of antidiarrheal oil oral emulsion on Diquat-induced injury

[0087] 1 test material

[0088] 1.1 Experimental animals and drugs

[0089] Thirty 7-week-old healthy female Wistar rats, weighing 200±5g, were purchased from Hubei Provincial Center for Disease Control and Prevention. Oral emulsion of dysentery oil was provided by Guangzhou Meirui Taike Bioengineering Technology Co., Ltd.

[0090] 1.2 Main reagents

[0091]Diquat: Chem Service, CAS6385-62-2; PCR Mixing System (PCR Master Mix): Bio-Rad Company; Taq DNA polymerase, dNTPs, DL2000Marker, DL15000Marker: Dalian Bao Biological Company (TAKALA); M -MLV reverse transcriptase, reverse transcription primer Oligo-(dT)20n, RNAse inhibitor, 10mMdNTPs, 10×TransFast Taq Buffer: Toyobo, Japan; TRIzol reagent (Invitrogin), chloroform, isopropanol, Absolute ethanol (Sinopharm); DEPC, bromophenol blue (Promega), EB (ethidium bromide, Sigma), Tris base (Amersham), agarose (Biow...

Embodiment 3

[0213] An oral emulsion for resisting the toxic reaction caused by diquat, which is composed of the following ingredients in percentage by weight: 2% of dysentery oil, 10% of hydrogenated castor oil, 0.5% of starch, 0.5% of glycerin, carboxymethyl cellulose Sodium 0.1%, deionized water as the balance.

[0214] Mix the dysentery oil and hydrogenated castor oil, and stir at a low speed for 5 minutes to obtain the oil phase; add starch and sodium carboxymethyl cellulose to deionized water, and use ultrasonic vibration to completely dissolve the two to obtain the water phase; Slowly pour the water phase into the oil phase, then add glycerin, and make milk under the condition of 20°C for 10 minutes to obtain the oral emulsion of anti-diarrhea oil.

[0215] The preventive and therapeutic methods are as follows: feed the livestock and poultry with the above-mentioned anti-dysentery oil oral emulsion, and the dosage is 50-100 ml.

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Abstract

The invention discloses a use of origanum vulgare oil in preparation of diquat toxicity-resistant drugs. A drug effect experiment shows that through oral administration, origanum vulgare can substantially improve intestinal tract antioxidant enzyme activity, reduce MDA content, reduce the amount of escherichia coli in chime, improve the amount of lactobacilli, inhibit high expression of an intestinal mucosa inflammatory factor, improve expression of tightly connected Occludin, reduce excess damage caused by diquat to intestinal mucosal cells and effectively protect an intestinal tract mechanical barrier. The invention provides an oral emulsion for resisting diquat-caused toxic reaction. The oral emulsion comprises 0.01-10% of origanum vulgare oil, 5-20% of an emulsifier, 0.1-10% of a physical stabilizer, 0.1-10% of a co-emulsifier, 0.01-1% of a thickening agent and the balance water. The oral emulsion has the characteristics of good drug absorption, fast action and high bioavailability, can mask the odor and poor taste of origanum vulgare oil and can improve drug compliance.

Description

technical field [0001] The invention belongs to the field of livestock breeding, and in particular relates to the use of anti-diquat-induced toxic drugs of anti-diquat and an oral emulsion used for anti-diquat-induced toxic reactions. Background technique [0002] Diquat is a conductive contact herbicide. According to my country's pesticide toxicity classification standard, Diquat is a moderately toxic herbicide. After being absorbed by green plants, diquat inhibits the electron transfer of photosynthesis. Under the induction of light, the bipyridine compound in the reduced state is quickly oxidized in the presence of oxygen to form active hydrogen peroxide. The accumulation of this substance makes the plant’s Cell membranes are destroyed. Diquat can be used as a desiccant for seed plants, and can also be used as a desiccant for potato, cotton, soybean, corn, sorghum, flax, sunflower and other crops. The rations of livestock and poultry are mainly grains and by-products fr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/31A61K9/107A61P39/02
CPCY02A50/30
Inventor 王若瑾袁保京金立志
Owner GUANGZHOU MERITECH BIO ENG TECH
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