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Fermentation medium for producing polyene macrolide antifungal antibiotics by fermentation of Streptomyces multichromogenes and preparation method thereof

A technology for producing Streptomyces multicolor and macrolides, which is applied in fermentation and other directions, can solve the problems of high product price, low production capacity, and difficulty in wide application, and achieves great application value, low cost, and easy source. Effect

Inactive Publication Date: 2018-03-27
JIAYING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the production capacity of polyene macrolide antibiotic-producing bacteria is low, and the product price is too high, so it is difficult to be widely used in agricultural production.
[0003] The antibacterial substances produced by Streptomyces polychromogenes have three absorption peaks at 350nm, 332nm and 317nm, and are broad-spectrum antifungal antibiotics of polyene macrolides, but so far no Streptomyces polychromogenes produce polyenes Report on Macrolide Antibiotics

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Medium composition: corn starch 40g / L, soybean meal 45g / L, potassium nitrate 5g / L, α-amylase 0.5g / L, yeast extract 1.5g / L, copper sulfate 0.02g / L, tap water to make up to 1000mL .

[0030] Preparation method: mix cornstarch and α-amylase, then add 5 times the volume of cornstarch in tap water to make a paste, heat at 70-80°C for 40 minutes to form an enzymatic hydrolysis solution of sugar carbon sources; The bean cake powder is dried and crushed, passed through a 30-mesh sieve, mixed with potassium nitrate, copper sulfate and yeast extract, and mixed with water 3 times the volume of the soybean cake powder, and then the pH of the solution is first adjusted with hydrochloric acid and sodium hydroxide value to 8.5, and then heated at 20-50°C for 180 minutes to form a nitrogen source material mixture; mix the sugar carbon source material enzymatic hydrolysis solution with the nitrogen source material mixture, then make up water, and finally oxidize with hydrochloric acid a...

Embodiment 2

[0033] Medium ingredients: wheat starch 60g / L, peanut cake powder 65g / L, ammonium nitrate 3g / L, disodium hydrogen phosphate 0.008g / L, ɑ-amylase 2g / L, yeast extract 3g / L, tap water to make up to 1000mL .

[0034] Preparation method: mix wheat starch and α-amylase evenly, then add tap water with 8 times the volume of wheat starch to make a paste, heat at 60-70 degrees for 80 minutes to form a carbohydrate carbon source enzymatic hydrolysis solution; then peanut The cake powder is dried and crushed, and then passed through a 100-mesh sieve, then mixed with ammonium nitrate, disodium hydrogen phosphate and yeast extract, and after adding water 10 times the volume of peanut cake powder and stirring evenly, first use hydrochloric acid and sodium hydroxide to Adjust the pH value of the solution to 10, and then heat at 50-100°C for 120 minutes to form a nitrogen source substance mixture; mix the sugar carbon source substance enzymatic hydrolysis solution with the nitrogen source subst...

Embodiment 3

[0037] Medium ingredients: sweet potato powder 80g / L, silkworm chrysalis powder 85g / L, ammonium sulfate 8g / L, sodium borate 0.05g / L, ɑ-amylase 4g / L, yeast extract 6g / L, tap water to make up to 1000mL.

[0038]Preparation method: mix sweet potato powder and α-amylase evenly, then add tap water with 8 times the volume of sweet potato powder to make a paste, heat at 50-60 degrees for 120 minutes to form carbohydrate carbon source material enzymatic hydrolysis solution; then Dry and crush the silkworm chrysalis powder, pass through a 60-mesh sieve, mix with ammonium sulfate, sodium borate and yeast extract, add 15 times the volume of silkworm chrysalis powder and stir evenly, then adjust the pH of the solution with hydrochloric acid and sodium hydroxide value to 10.5, and then heated at 100-150°C for 100 minutes to form a nitrogen source material mixture; mix the sugar carbon source material enzymatic hydrolysis solution with the nitrogen source material mixture, then make up water...

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Abstract

The invention discloses a fermentation medium for producing polyene macrolide antifungal antibiotics by fermentation of Streptomyces multichromogenes and a preparation method thereof, belonging to the technical field of fermentation engineering and biological pesticides. The fermentation medium includes sugar carbon source, organic nitrogen source, inorganic nitrogen source, inorganic salt, yeast extract and amylase; the preparation method comprises the following steps: (1) after mixing the carbohydrate carbon source and amylase, add water and heat to form carbohydrate carbon source material enzyme solution; (2) mix organic nitrogen source, inorganic nitrogen source, inorganic salt and yeast extract and add water to heat to form a nitrogen source material mixture; (3) mix carbohydrate carbon source material enzymatic hydrolysis solution and nitrogen source material mixture , then make up water, and finally adjust the pH value of the solution to 3 to 10 with hydrochloric acid and sodium hydroxide; the present invention aims to provide a fermentation medium and its Preparation method: used for fermenting Streptomyces chromogenes to produce polyene macrolide antifungal antibiotics.

Description

technical field [0001] The invention relates to a fermentation medium, more specifically, to a fermentation medium for producing polyene macrolide antifungal antibiotics by fermentation of Streptomyces chromogenes. The present invention also relates to a method for preparing the above-mentioned fermentation medium. Background technique [0002] Polyene macrolide antibiotics are broad-spectrum antifungal substance antibiotics with strong antibacterial effect on a variety of fungi and no antibacterial effect on bacteria, and are world-recognized safe, broad-spectrum, and highly effective antibacterial active substances, which cannot be used by the human body stomach and intestinal absorption. Natamycin and aureomycin, which are polyene macrolides, are clinically used to treat skin diseases caused by fungi, and there is almost no resistance to them after being used for more than half a century. In recent years, they have been widely used as dairy products at home and abroad. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/08
Inventor 张镜
Owner JIAYING UNIV
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