Production technology of high purity yak serum albumin

A serum albumin and production process technology, applied in the preparation method of peptides, organic chemistry, peptides and other directions, can solve the problems of time-consuming, laborious, flammable ethanol, and high content of impurity proteins, and achieve easy process operation, high reliability and high reliability. The effect of safety and simple preparation process

Inactive Publication Date: 2016-03-09
QINGDAO ARCTIC YAK BIOLOGICAL TECH CO LTD
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The salting-out method is the most traditional production method. The principle of this method is relatively simple, but ammonium sulfate is corrosive to the equipment. The whole process is time-consuming, and the amount of ammonium sulfate is large. The production rate is generally 0.8~1.0%, which leads to low production efficiency and high cost of this method; the use of cold ethanol precipitation method also has some shortcomings: ethanol is a non-specific precipitant, although the production efficiency of BSA is remarkable, the product purity is not very high , as an organic solvent, ethanol may also cause protein aggregation or denaturation. In order to avoid protein denaturation, it must be carried out in a low-temperature environment of -20°C. The investment in equipment and workshops is large, and ethanol is flammable

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0030] Example 1

[0031] Take 56000mL of fresh yak blood from the yak herd without infection after strict quarantine, add it to 6220mL of 4.2% sodium citrate, 0.9% sodium chloride solution, put the blood in a cold storage at 3℃ for 10 hours, the plasma and red blood cells Separate the layers, use the siphon method to suck the blood plasma, and use the lower blood cells separately; put the above separated plasma in a centrifugal system with a refrigeration device, set the temperature of the centrifugal system to 5°C, turn on the centrifuge and centrifuge at 5000 rpm Centrifuge for 50 minutes to collect plasma. The plasma is then filtered with a 0.22μm microporous membrane to obtain about 28,000 mL of refined plasma;

[0032] Put the purified plasma into the interlayer reaction tank, and heat the interlayer tank to 70°C; take 93.1g sodium caprylate and 252g sodium chloride, add to 28000mL purified water to dissolve, after dissolution, add the solution to the purified plasma, then a...

Example Embodiment

[0033] Example 2

[0034] After strict quarantine, 40,000 mL of fresh yak blood from a herd of yak-free infections was taken and added to 4450 mL of a 4.2% sodium citrate and 0.9% sodium chloride solution. After the blood was placed in a cold storage at 8°C for 8 hours, the plasma and red blood cells Separate the layers, use the siphon method to suck the plasma, and use the lower blood cells separately; put the above-mentioned separated plasma in a centrifugal system with a refrigeration device, set the temperature of the centrifugal system to 8℃, turn on the centrifuge and centrifuge at 5000 rpm / Centrifuge for 45 minutes to collect plasma. The plasma is then filtered with a 0.22μm microporous membrane to obtain about 20,000 mL of refined plasma;

[0035] Put the purified plasma into the interlayer reaction tank, and heat the interlayer tank to 70℃; take 66.5g sodium caprylate and 180g sodium chloride, add to 20000mL purified water to dissolve, after dissolution, add the solution...

Example Embodiment

[0036] Example 3

[0037] After strict quarantine, 50,000 mL of fresh yak blood from a herd of yak-free infections was added to 5560 mL of a solution containing 3.8% sodium citrate and 0.9% sodium chloride. After the blood was placed in a cold storage at 5°C for 8 hours, the plasma and red blood cells Separate the layers, use the siphon method to absorb the plasma, and use the lower blood cells separately; put the above separated plasma in a centrifugal system with a refrigeration device, set the temperature of the centrifugal system to 10℃, turn on the centrifuge and centrifuge at 3000 rpm Centrifuge for 60 minutes to collect plasma. The plasma is then filtered with a 0.22μm microporous membrane to obtain about 25,000 mL of refined plasma;

[0038] Put the refined plasma into the interlayer reaction tank, and heat the interlayer tank to 70℃; take 83.2g sodium caprylate and 225.0g sodium chloride, add to 25000mL purified water to dissolve, after dissolution, add the solution to th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a production technology of high purity yak serum albumin. The production technology comprises the following steps: adding sodium citrate and sodium chloride into yak blood, separating plasma and erythrocyte, subjecting plasma to centrifugation, filtering and extraction, putting the plasma extract in an interlayer reaction pot, heating the pot, adding sodium caprylate, sodium chloride, and ethanol/methanol, slowing stirring, adding hydrochloric acid for twice to adjust the pH value, carrying out centrifugation, adding a precipitating reagent into the supernate, grinding the supernate into paste, stirring, freezing the paste for a night, carrying out centrifugation, collecting the precipitate, adding water to dissolve precipitate, adjusting the pH value by a sodium hydroxide solution, allowing the solution to stand still for a while, carrying out centrifugation, saving the supernate, filtering the supernate, inactivating the virus, and freeze-drying the supernate to obtain the finished product. The preparation technology is simple, the used materials are few, the technological procedure is short, the operation is easy, the product yield is high, the cost is low, and the product quality is good. The massive and automatic production can be easily realized. Moreover, the product is more reliable and safer.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a production process of high-purity yak serum albumin. Background technique [0002] Bovine Serum Albumin (BSA) is a globulin in bovine serum, which contains 583 amino acid residues, a molecular weight of 66.43kDa, an isoelectric point of 4.7, a nitrogen content of 16%, and a sugar content of 0.08%. , the fat content is only 0.2%; albumin can be combined with various cations, anions and other small molecular substances. BSA is a biochemical reagent widely used in many disciplines of biology and in various fields of medicine and pharmacy. For example, it is used as a blocking reagent in western blot, Albumin in the blood mainly plays the role of maintaining osmotic pressure, pH buffering, carrier and nutrition; in the serum-free culture of animal cells, adding albumin can play the role of physiological and mechanical protection and carrier. [0003] There are many preparation methods...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K1/34C07K1/30
Inventor 许卫红
Owner QINGDAO ARCTIC YAK BIOLOGICAL TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap