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A Pseudomonas putida and Its Application in Promoting the Growth of A.

A technology of Pseudomonas putida and Cinnamomum, which is applied in the field of biological fertilizers and microbial fertilizers, can solve the problems of no growth-promoting bacteria of Cinnamon, achieve excellent strain resources, promote growth and development, and promote seed germination and growth. Effect

Active Publication Date: 2018-10-23
长治学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, there are no reports on the growth-promoting bacteria of Lianxiang tree at home and abroad

Method used

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  • A Pseudomonas putida and Its Application in Promoting the Growth of A.
  • A Pseudomonas putida and Its Application in Promoting the Growth of A.
  • A Pseudomonas putida and Its Application in Promoting the Growth of A.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Determination of the phosphorus-dissolving ability of LWPZF liquid.

[0027] NBRIP medium: glucose 10g, Ca 3 (PO 4 ) 2 5.0g, MgCl 2 5g, MgSO 4 ·7H 2 O 0.25g, KCl0.2g, (NH 4 ) 2 SO 4 0.1g, distilled water 1000mL, pH 7.0.

[0028] The LWPZF strain activated twice was inoculated into NB medium (3.0 g of beef extract, 10.0 g of peptone, 5.0 g of sodium chloride, 1000 mL of distilled water, pH 7.2-7.4), and cultured at 30°C for 18-24 hours to make a seed solution. 0.5mL seed solution was inoculated into a 100mL Erlenmeyer flask containing 50mL NBRIP culture solution, and the NBRIP medium inoculated with 0.5mL blank seed solution was used as a control. Each treatment was replicated three times, 30°C, 180r / min shaking culture for 4 days. The fermented liquid was centrifuged at 10000r / min for 10min at 4°C, and the molybdenum-antimony anti-colorimetric method was used to measure the soluble phosphorus content in the fermented liquid ( figure 1 ).

[0029...

Embodiment 2

[0029] from figure 1 It can be seen from the figure that the soluble phosphorus content in the fermentation liquid inoculated with LWPZF strain was 742.04 mg·L -1 , is the control (32.81mg·L -1 ) 22.6 times. In summary, the LWPZF strain has a strong ability to degrade calcium phosphate. Example 2: Determination of the ability of LWPZF to produce auxin.

[0030] King's B medium: peptone 20g, MgSO 4 ·7H 2 O 1.5g, K 2 HPO 4 1.5g, glycerol 10mL, agar 15g, after adjusting the pH value to 7.0, add to 1000mL.

[0031] S1 colorimetric solution: accurately weigh 12g FeCl 3 Dissolve in 300mL of deionized water, then slowly add 429.7mL of concentrated sulfuric acid, and dilute to 1L after cooling.

[0032] Prepare IAA standard solutions with concentrations of 1, 4, 6, 8, 10, 12, 14, 16, 18, and 20 mg / L, and mix them with S1 reagent at a volume ratio of 1:1, place them at room temperature in the dark for 30 minutes, and then Determine the OD of each concentration 530nm (The 1:...

Embodiment 3

[0035] Example 3: Determination of the ability of LWPZF to promote growth of cucumber seeds.

[0036] The cucumber seeds were treated with 10% hydrogen peroxide for 20 minutes, washed 5-6 times with sterile water, and dried for later use.

[0037] The LWPZF strain was inoculated in NB liquid medium, and cultured on a shaker at 28°C for 24-36 hours, so that the bacterial concentration reached 10 9 cfu / mL. The bacterial suspension was serially diluted to 10 -7 , divide the bottom of the sterile petri dish into 8 equal parts with a marker pen, mark CK, -1, -2, -3, -4, -5, -6, -7 in turn, place 4~ 6 pieces of filter paper, put sterile absorbent cotton in the middle, put the processed cucumber seeds on the filter paper, drop 100 μl of LWPZF bacterial suspension corresponding to the dilution in the center of the filter paper in each area of ​​the bottom of the dish, drop in the CK area, etc. Amount of sterile water was used as a control. 28°C, cultivated under light, and observe...

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Abstract

The invention discloses a Pseudomonas putida, which is classified as Pseudomonas putida and has a strain number of LWPZF. It has been preserved in the China Center for Type Culture Collection CCTCC, and the preservation number is CCTCC NO: M 2016008. The deposit date is January 5, 2016. The invention also discloses the application of the above-mentioned Pseudomonas putida in promoting the growth of the japonica. The LWPZF strain has a strong ability to decompose inorganic phosphorus and auxin secretion; it can significantly promote the germination of cucumber seeds; the LWPZF bacterial agent was inoculated with the seedlings of Lianxiang tree, and the results showed that the bacterial agent can significantly promote the growth of Lianxiang tree seedlings. At the same time, the strain has a certain antagonistic effect on plant pathogens such as Fusarium oxysporum, rice sheath blight, Rhizoctonia and rice blast fungus. Therefore, the present invention provides excellent bacterial strain resources for the development of the special biological fertilizer for the syringa japonica.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers in the field of biological fertilizers, and in particular relates to a pseudomonas putida and its application in promoting the growth of japonica. Background technique [0002] Cercidiphyllum japonicum Sieb.Et Zucc. is a deciduous tree belonging to Cercidiphyllum japonicum Sieb.Et Zucc. Lianxiang tree remnants are mainly distributed in the subtropical and warm temperate regions of China. This species is a relic single family plant of the ancient tropical plants of the Tertiary Period, and it is an ancient and primitive woody plant. Dioecious, fruit less, its seedlings are vulnerable to natural conditions such as disease and insect pests, rainstorms, so it is difficult to grow under natural conditions, and there are very few young trees under the forest. Lianxiang tree resources are scarce and are on the verge of extinction. Therefore, it is included in the "List of Rare and Endang...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00A01P3/00C12R1/40
CPCA01N63/00C12N1/205C12R2001/40
Inventor 任嘉红张桂萍晋婷婷白凤麟彭卿王莹
Owner 长治学院
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