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Enzymatic hydrolysis preparation technology of bifidobacteria and lactic acid bacteria mixed fermented bacteria and application thereof

A technology of mixed fermentation bacteria and bifidobacteria, which is applied in the field of enzymatic hydrolysis preparation process of mixed fermentation bacteria of bifidobacteria and lactic acid bacteria, can solve the problems of ineffective treatment and health care, low equipment utilization rate and bacteria yield, and product use effect No obvious problems, etc., to achieve the effect of improving human immunity, improving self-disease resistance, photosynthesis and stress resistance

Inactive Publication Date: 2016-05-11
山东环亿生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Live bifidobacteria and their fermentation metabolites play an important role in human health, but the current production method of bifidobacteria is mainly anaerobic fermentation, but this method has a long production cycle, large equipment investment, and low equipment utilization rate. The yield of bacteria is low, the effective substance is single, the effect of the product is not significant, and the scope of application is small. Because the product is extremely sensitive to oxygen and temperature, the resistance to acid is poor, it is difficult to maintain viable bacteria, and the shelf life is short, so it cannot be used effectively. treatment, health care

Method used

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  • Enzymatic hydrolysis preparation technology of bifidobacteria and lactic acid bacteria mixed fermented bacteria and application thereof
  • Enzymatic hydrolysis preparation technology of bifidobacteria and lactic acid bacteria mixed fermented bacteria and application thereof
  • Enzymatic hydrolysis preparation technology of bifidobacteria and lactic acid bacteria mixed fermented bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Prepare amplified culture solution

[0027] Preparation of medium: 10 grams of walnut kernels, 10 grams of peanut kernels, 5 grams of sodium caseinate, 3 grams of L-arginine, 3 grams of tomato powder, 6 grams of beef extract, 2 grams of yeast extract powder, 5 grams of peptone, Ammonium Citrate 0.1g, Serine 0.6g, Sodium Chloride 3g, Lactose 5g, Glucose 8g, Sodium Acetate 8g, Anhydrous Calcium Chloride 0.1g, Dipotassium Hydrogen Phosphate 3.2g, 500,000 Units of Lipase 10 gram, 100,000 units of fig enzyme 10 grams, 1000 milliliters of tap water, PH natural.

[0028] Operation: Pretreatment of raw materials:

[0029] First take 300ml of water in the formula, put it into a grinder, then put in walnut kernels, peanut kernels, sodium caseinate, and anhydrous calcium chloride, then start the grinder to grind it into a slurry, filter and discard the residue to collect the slurry , and then add the remaining water in the formula to the slurry, heat the slurry to 55°C±2°C, add ...

Embodiment 2

[0032] Preparation of Primary Seed Culture Solution

[0033] Recipe: 20 grams of walnut kernels, 20 grams of peanut kernels, 10 grams of sodium caseinate, 5 grams of L-arginine, 6 grams of tomato powder, 8 grams of beef extract, 3 grams of yeast extract powder, 3 grams of peptone, amine citrate 0.15 grams, 0.8 grams of serine, 0.4 grams of gluconic acid, 3 grams of sodium chloride, 10 grams of lactose, 15 grams of glucose, 6 grams of sodium acetate, 0.1 grams of anhydrous calcium chloride, 2 grams of dipotassium hydrogen phosphate, 500,000 units of fat Enzyme 10 grams, 100,000 units fig enzyme 10 grams, tap water 1000 ml, PH natural.

[0034] Operation: Pretreatment of raw materials: first take 600ml of water in the formula, put it into the grinder, then add walnut kernels, peanut kernels, sodium caseinate, anhydrous calcium chloride, and then start the grinder to make a slurry shape, filter the discarded residue to collect the slurry, then add the remaining water in the form...

Embodiment 3

[0036] Prepare the first-class seed solution

[0037] Pack the first-grade seed culture solution into triangular flasks according to the amount used, then tie up the bottle mouth with eight layers of gauze and two layers of kraft paper, put it in a medical sterilizer for sterilization, and open the exhaust valve on the sterilizer first before sterilization , and close when a small amount of steam is released. When the pressure on the sterilizer reaches 0.05 MPa, open the exhaust valve to exhaust for 6-8 minutes, then close the exhaust valve and continue heating. When the pressure reaches 0.1-0.14 MPa, keep it for 25 minutes, and then turn off After the sterilization is completed, leave the heating source and let it cool down naturally. When the pressure gauge returns to zero, turn on the sterilizer, take out the sterilized triangular flask culture medium and put it in an ultra-clean workbench to let it cool down to 37°C naturally, and then culture the above The expanded strai...

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Abstract

The invention relates to the field of biological bacteria, and particularly provides an enzymatic hydrolysis preparation technology of bifidobacteria and lactic acid bacteria mixed fermented bacteria and application thereof. The preparation technology comprises the steps of: preparing an expanding bacteria liquid and a first-grade seed medium liquid to obtain a first-grade bacteria liquid; then conducting mixed cultivation on the first-grade bacteria liquid and a seed medium in a seeding tank to obtain seeding tank bacteria; then conducting mixed culture on the seeding tank bacteria and a fermentation tank medium, and fermenting by using an inducing fluid to obtain a fermentation liquid; after the fermentation, centrifuging the fermentation liquid to obtain wet bacterial sludge which is mixed fermentation bacterial sludge of bifidobacterium and lactic acid bacteria. The bifidobacterium mixed bacteria prepared by the invention has high activity and long quality guarantee period, makes the bifidobacteria produce more effective substances to the greatest degree and avoid the influences of oxygen, moisture, light and protective matrix on bifidobacteria.

Description

technical field [0001] The invention relates to the field of biological bacteria, in particular to a preparation process of bifidobacterium lactic acid bacteria mixed fermentation bacteria enzymolysis method and its application. Background technique [0002] Bifidobacterium is a physiological bacterium that exists in the human body. It is the most worthy of attention and research among the beneficial bacteria for the human body. It is inseparable from the health of the human body. It can be said that it is a magic weapon for human health bestowed by nature. [0003] Bifidobacteria are one of the most important normal flora in the intestinal tract of humans and animals. They can grow on the intestinal mucosa to form a membrane flora, forming a biological barrier, so that the invading pathogenic bacteria lose their growth in the intestinal tract The foothold of reproduction, bifidobacteria grow and reproduce in the intestinal tract to produce acetic acid, lactic acid, acetic ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/01C12R1/225
CPCA01N63/10C12N1/20
Inventor 张起凡曹崇仁朱友峰
Owner 山东环亿生物科技有限公司
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