Preparation method and application of a kind of electrochemiluminescence furazolidone biosensor

A biosensor, furazolidone technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, scientific instruments, etc., can solve the problems of poor conductivity, unfavorable practical application, low sensitivity of electrochemiluminescence sensor, etc. Achieve the effect of solving poor electrical conductivity, improving strength and stability, and increasing electrochemiluminescence intensity

Inactive Publication Date: 2018-03-30
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the poor conductivity of titanium dioxide also limits the sensitivity of electrochemiluminescence sensors constructed from a single titanium dioxide nanomaterial, which is not conducive to practical applications.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Mn-TiO 2 / g -C 3 N 4 preparation

[0036] First, take 0.8 mmol of manganese salt and add it to 5 mL of tetrabutyl titanate. During stirring, slowly add 0.5 mL of hydrofluoric acid, and react in the reactor at 160 °C for 24 hours. After cooling to room temperature, use ultrapure water After centrifuging and washing with absolute ethanol for three times, vacuum-dry at 50°C; secondly, mix 150 mg of the dried solid with 400 mg of melamine, and grind it into a powder; then, put the ground powder into a muffle furnace at a heating rate of 1 ℃ / min, calcined at 480 ℃ for 5 hours; finally, the calcined powder was cooled to room temperature to obtain Mn-TiO 2 / g -C 3 N 4 ;

[0037] Described manganese salt is manganese sulfate.

Embodiment 2

[0038] Example 2 Mn-TiO 2 / g -C 3 N 4 preparation

[0039] First, take 1.0 mmol of manganese salt and add it to 5 mL of tetrabutyl titanate. During the stirring process, slowly add 0.6 mL of hydrofluoric acid and react in the reactor at 180 °C for 21 hours. After cooling to room temperature, use ultrapure water After centrifuging and washing with absolute ethanol three times, vacuum-dry at 50°C; secondly, mix 200 mg of the dried solid with 400 mg of melamine, and grind it into a powder; then, put the ground powder into a muffle furnace, and the heating rate is 2 ℃ / min, calcined at 520 ℃ for 2 hours; finally, the calcined powder was cooled to room temperature to obtain Mn-TiO 2 / g -C 3 N 4 ;

[0040] Described manganese salt is manganese chloride.

Embodiment 3

[0041] Example 3 Mn-TiO 2 / g -C 3 N 4 preparation

[0042] First, take 1.2 mmol of manganese salt and add it to 5 mL of tetrabutyl titanate. During stirring, slowly add 0.8 mL of hydrofluoric acid, and react in the reactor at 200 °C for 18 hours. After cooling to room temperature, use ultrapure water to After centrifuging and washing with absolute ethanol three times, vacuum-dry at 50°C; secondly, mix 250 mg of the dried solid with 400 mg of melamine, and grind it into powder; then, put the ground powder into a muffle furnace, and the heating rate is 3 ℃ / min, calcined at 560 ℃ for 0.5 hours; finally, the calcined powder was cooled to room temperature to obtain Mn-TiO 2 / g -C 3 N 4 ;

[0043] Described manganese salt is manganese nitrate.

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Abstract

The invention discloses a preparation method of an electrochemiluminescent furazolidone biosensor. It belongs to the technical field of new nanometer functional materials and biosensors. The present invention first prepares a novel two-dimensional nanocomposite material Mn-TiO2 / g-C3N4, utilizes the good biocompatibility and large specific surface area of ​​the material, loads furazolidone antibody, and then cross-links through glutaraldehyde Acting on horseradish peroxidase, when detecting, because horseradish peroxidase can catalyze hydrogen peroxide to generate oxygen in situ, and then electrochemically react with the bottom liquid to produce high and stable electrochemiluminescence signal, and then use the effect of the specific quantitative combination of antibody and antigen on the electron transport ability, so that the electrochemiluminescent signal is correspondingly reduced, and finally a low-cost, high-sensitivity, good specificity, rapid detection, and simple preparation method for the detection of furazolidone was obtained. Electrochemiluminescent biosensors.

Description

technical field [0001] The invention relates to a preparation method of an electrochemiluminescent furazolidone biosensor. It belongs to the technical field of new nanometer functional materials and biosensors. Background technique [0002] Furazolidone (furazolidone) is a nitrofuran antibiotic, which is a broad-spectrum antibacterial drug. When used as a veterinary drug, furazolidone has good medicinal effects on the prevention and treatment of certain protozoan diseases, saprolegniasis, bacterial gill rot, red skin disease, and hemorrhagic diseases. In the breeding industry, furazolidone can be used to treat intestinal infections in livestock and poultry, such as piglet yellow and pullorum. In aquaculture, furazolidone has a certain effect on the infection of Myxosoma cerebri in Salmonidae. However, the Ministry of Agriculture of my country lists furazolidone as a prohibited drug and shall not be detected in animal food. FDA also banned the use of nitrofurans (includin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N33/543
CPCG01N21/76G01N33/5438
Inventor 张勇庞雪辉李燕王晓东魏琴
Owner UNIV OF JINAN
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