A kind of degradable polyaniline/porous silicon nanocomposite material and its preparation method and application
A nano-composite material, porous silicon technology, applied in wave energy or particle radiation treatment materials, medical preparations with inactive ingredients, medical preparations containing active ingredients, etc., can solve the problem of hindering combination therapy, low anti-cancer drug loading ability, poor water solubility, etc., to achieve stable photothermal ability, obvious synergistic treatment effect of chemotherapy and hyperthermia combination, and good water solubility.
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Embodiment 1
[0035] Material preparation: Cut the silicon wafer into a square of 2cm×2cm, put it in a solution of concentrated sulfuric acid (98%):hydrogen peroxide=3:1 (volume ratio), soak it for 30 minutes under the condition of heating at 80°C to remove the surface organic contamination, then rinse several times with copious amounts of deionized water. Mix hydrofluoric acid with a concentration of 40% and deionized water at a volume ratio of 1:1 in a plastic beaker, then put the silicon wafers rinsed with deionized water into the mixture, and rinse each piece for 30 seconds to remove the surface oxide layer.
[0036] The silicon wafer is the anode, the platinum wire is the cathode, and the etching solution is a mixed solution made of hydrofluoric acid and absolute ethanol at a volume ratio of 3:1. The substrate is a pre-treated P-type silicon wafer, and a layer of aluminum film is placed on the back to form a good ohmic contact. 2 , and the etching time was 15 minutes to etch the sili...
Embodiment 2
[0042] Example 2 Cell Application
[0043] The MTT assay was used to evaluate the viability of 4T1 cells. The cells were incubated with DOX, PANi-PSiNPs, and DOX@PANi-PSiNPs, respectively, and then irradiated with laser light for 20 minutes (for a control experiment, the material was lighted and not lighted). Incubated for different times (2, 4, 10 or 20 hours). Such as Image 6 , after incubation, DOX@PANi-PSiNPs combined with laser showed the highest cytotoxicity compared with other groups, the results showed that compared with the control group, under the same conditions, the cell survival rate of chemotherapy only was 37.1% , the survival rate of cells treated with photothermal therapy alone was 62.2%, while the cell survival rate when chemotherapy was combined with photothermal therapy was 23.1%.
Embodiment 3
[0044] Embodiment 3 application in vivo
[0045] will be 10 6 Mouse breast cancer cells (4T1) were injected into the outer right leg of Balb / C mice, when the tumor size reached 50-70mm 3 Intratumoral injection materials: (1) phosphate-buffered saline (PBS) + NIR (near-infrared laser) (control), (2) doxorubicin (DOX) + NIR, (3) PANi-PSiNPs + NIR, ( 4) DOX@PANi-PSiNPs, (5) DOX@PANi-PSiNPs+NIR, the injection dose is 1mg / kg DOX, 10mg / kg PANi-PSiNPs or 10mg / kg DOX@PANi-PSiNPs, NIR laser power is 1.4W / cm 2 , the irradiation time was 10 minutes, and the thermal imaging camera was used to detect the temperature change of the tumor in mice. For the detection data of the thermal imaging camera, see Figure 7 , after 10 minutes of NIR laser irradiation, the temperature of the tumor area injected with PANi-PSiNPs and DOX@PANi-PSiNPs increased to about 45 °C, while the temperature of the tumor area in the other groups was about 30 °C, the results showed that the injection of PANi-PSiNP...
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