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Magnetic shell-core structural nanoparticles and preparation method and application thereof

A magnetic nanoparticle and nanoparticle technology, applied in the field of glycosylated proteins, to achieve the effect of improving chemical resistance and stability, improving stability and chemical resistance, and being easy to repeat

Inactive Publication Date: 2017-04-05
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are various methods for the separation and enrichment of glycoproteins, including trap chemical enrichment method, phenylboronic acid method, lectin affinity method, hydrophilic interaction chromatography, etc.; in addition, the inorganic substrates modified by materials are also abundant. Various, including silica, graphite carbon, activated carbon, metal particles and metal oxides, etc., but there are still certain limitations in practical applications

Method used

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  • Magnetic shell-core structural nanoparticles and preparation method and application thereof
  • Magnetic shell-core structural nanoparticles and preparation method and application thereof
  • Magnetic shell-core structural nanoparticles and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Preparation of dipeptide functionalized polymers

[0049] The preparation steps of dipeptide (L-Asp-L-Phe) functionalized polymer are as follows: 1 mol of methyl esterified dipeptide L-Asp-L-Phe is dissolved in chloroform, and triethylamine is added dropwise to alkalinity, in In an ice-water bath, 1.2-1.5 mol of chloroacetyl chloride was added dropwise and reacted for 12 hours to obtain a chloroacetylated dipeptide. Dissolve 1 g of chloroacetylated dipeptide, polyethyleneimine (PEI, with a degree of polymerization ranging from 5 to 200,000), potassium carbonate, and sodium iodide in 100 mL of anhydrous N,N-dimethylformamide solution, After 24 hours of reaction in the dark at room temperature under the protection of nitrogen, the dipeptide functionalized polymer was obtained by purification and dialysis.

[0050] The following is a schematic diagram of the preparation process of the dipeptide functionalized polymer:

[0051]

Embodiment 2

[0053] Porous silica-coated magnetic nanoparticles (Fe 3 o 4 @SiO 2 @mSiO 2 Indicates the preparation of

[0054] The magnetic nanoparticles of the present embodiment select Fe 3 o 4 , it can be understood that other types of magnetic nanoparticles are also suitable for the present invention.

[0055] 1) Preparation of Fe 3 o 4 Magnetic nanoparticles: 0.3M ferric chloride hexahydrate and 0.2M ferrous sulfate heptahydrate in aqueous solution (H 2 O) fully dissolved in, under the protection of nitrogen, add the ammoniacal liquor (NH 3 ·H 2 O), and fully stirred at a temperature of 85 ° C. After 2 hours, the magnetic nanoparticles (Fe 3 o 4 ) were separated and washed thoroughly with ethanol;

[0056] 2) Preparation of Fe 3 o 4 @SiO 2 : the Fe 3 o 4 Magnetic nanoparticles are dispersed in a mixed solution of ethanol, deionized water, ammonia water and orthosilicate (80:17:2.5:0.5 by volume), and the obtained 2g / L magnetic nanoparticles are fully stirred at room t...

Embodiment 3

[0059] Preparation of magnetic core-shell nanoparticles

[0060] 1) Magnetic nanoparticles (Fe 3 o 4 @SiO 2 @mSiO 2 ) 0.1g dispersed in 100mL toluene, adding siloxane isothiocyanate modifier accounting for 5% of the volume ratio of the aforementioned solution, reflux reaction at 85°C for 24 hours, and washing with ethanol three times;

[0061] 2) Mix and disperse the dipeptide functionalized polymer obtained in Example 1 and the siloxane-modified magnetic nanoparticles obtained in the above step 1) in 100mL N,N-dimethylformamide solution, and add triethyl amine to alkaline, and reacted for 24 hours, centrifuged and washed with ethanol three times to obtain magnetic core-shell structure nanoparticles.

[0062] figure 1 Schematic diagram of the structure of the magnetic core-shell nanoparticle. The magnetic core-shell structure nanoparticles include a plurality of magnetic nanoparticles 1, a solid silica shell 2 covering a plurality of magnetic nanoparticles 1, a porous si...

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Abstract

The invention provides magnetic shell-core structural nanoparticles and a preparation method and application thereof. The magnetic shell-core structural nanoparticles comprise a plurality of magnetic nanoparticles, silica shell layers coating the plurality of magnetic nanoparticles, porous silica shell layers coating the silica shell layers and dipeptide-functionalized polymer grafted to surfaces of the porous silica shell layers. The method comprises: preparing magnetic nanoparticles, coating the silica shell layers and the porous silica shell layers with the magnetic nanoparticles, performing modification with a siloxane crosslinking modifier, and finally grafting the surfaces of the porous silica shell layers, which is modified with siloxane, with the dipeptide-functionalized polymer to obtain the magnetic shell-core structural nanoparticles. The magnetic shell-core structural nanoparticles can be applied to enrichment and separation of glycosylated protein. The magnetic shell-core structural nanoparticles successfully combine with dispersive solid-phase extraction, thereby achieving high-selectivity, good-repeatability and high-throughput enrichment of glycosylated protein and polypeptide materials.

Description

technical field [0001] The invention relates to the field of glycosylated proteins, in particular to a magnetic core-shell structure nanoparticle and its preparation method and application. Background technique [0002] As an important part of various biological macromolecules in living organisms, sugar groups participate in a series of rich and diverse and important life activities. Among them, especially in the process of protein glycosylation, the type, arrangement order and different combination methods of sugar groups will play an important role in the conformation of proteins and their physiological processes, including cell adhesion and information transmission, cell Proliferation and differentiation, immune response, etc. Moreover, in the occurrence and development of many diseases, such as tumors, cardiovascular diseases, immune diseases and neurodegenerative diseases, it is also closely related to the abnormality of protein glycosylation. Therefore, glycosylated ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/28B01J20/32C07K1/14
CPCB01J20/06B01J20/103B01J20/267B01J20/28009B01J20/3278B01J20/3295C07K1/145
Inventor 卿光焱孙涛垒陈中慧张政楷
Owner WUHAN UNIV OF TECH
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