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Method for removing pollutants from sewage containing arsenic

A technology for pollutants and sewage, applied in the directions of water pollutants, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of ecological environment pollution, complexity, abnormal operation, etc., and achieve the effect of good application prospects.

Active Publication Date: 2017-04-26
湖北康创科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The composition of industrial sewage is relatively complex, especially a large number of artificially synthesized compounds entering the environment. Such substances are mainly ammonia nitrogen, sulfide and phosphorus-containing compounds. Due to the complexity of the structure of these substances, they cannot be decomposed and utilized by microorganisms in a short time. The traditional wastewater treatment method using activated sludge to cultivate and domesticate microorganisms can no longer effectively remove these pollutants. These substances have accumulated in the environment for a long time, causing great pollution to the ecological environment on which we live, and affecting human physical and mental health. bring great harm
A considerable number of industrial polluting enterprises in our country would rather be punished than invest in wastewater treatment. Even if there is a sewage treatment plant, the operation is extremely abnormal.

Method used

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  • Method for removing pollutants from sewage containing arsenic

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for removing pollutants in sewage, comprising the steps of:

[0027] Preparation of the composite bacterial agent: mix the mixed bacterial liquid and the carrier according to the weight ratio of 1:1, stir evenly, then let it stand for 6 hours, and finally place it at 4°C for low-temperature drying, and control the water content at 6% after drying, to obtain final product; The carrier is obtained by mixing bamboo charcoal, chitosan and diatomaceous earth according to the mass ratio of 2:2:1; the above-mentioned mixed bacteria liquid is mixed by the following raw material bacteria in parts by weight: 10 parts of Rhodococcus, 9 parts of Thiobacillus denitrificans, 7 parts of Pseudomonas stutzeri, 6 parts of Sphingomonas, 5 parts of Bacillus pumilus, 2 parts of Phanerochaete chrysosporium; 8 pieces / ml.

[0028] Sewage pretreatment: First, the sewage NH3-N is 300mg / L, sulfide is 80mg / L, phosphorus-containing pollutants are 70mg / L, and arsenic content is 20mg / ml. T...

Embodiment 2

[0031] Utilize DNAMAN software to design primers, respectively add enzyme cutting sites, synthesize the nucleotide sequence PHY gene according to the amino acid sequence NP_745067.1 whole gene, obtain the target fragment through amplification, and obtain the target gene PHY through PCR amplification (simultaneously The corresponding mutation sites 62K / G, 80E / V, 93V / H, 120R / I, 131F / P, 143Q / S, 152P / Q, 169G / I, 179Q / A, 192Q / G, 241W were identified by multiple PCR / S, 258R / T, and 263R / P were introduced into the gene sequence to obtain different mutant genes), and the PCR product was double-digested with the added restriction sites, and cloned with the same double-digested expression vector pEASY Connected, the successfully verified recombinant plasmid was transformed into the Rhodococcus ATCC15906 strain to obtain arsenic-degrading genetically engineered bacteria.

Embodiment 3

[0032] Example 3 Verification of the sewage treatment effect of Rhodococcus genetically engineered bacteria and other several bacterial agents

[0033] According to the method of Example 1, the corresponding sewage treatment experiment was carried out, and the sewage and the sewage of Example 1 belonged to the same batch and had the same concentration of pollutants. Wherein each component composition of bacterial agent is identical with embodiment 1.

[0034]The genetically engineered bacteria of different mutation sites described in Example 2 were used to carry out the reaction of removing arsenic, and it was found through experiments that 62K / G, 80E / V, 93V / H, 120R / I, 131F / P, 143Q / S , 152P / Q, 169G / I, 179Q / A, 192Q / G, 241W / S, 258R / T, and 263R / P mutants all have significantly enhanced effects compared to the original strain. The total treatment time is not more than 4 days, and the result is as follows: the sewage is the same batch of sewage, so as to ensure the same conditions...

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Abstract

The invention relates to a method for removing pollutants from sewage containing arsenic, and the method includes the following steps: first, the sewage is subjected to solid-liquid separation by a solid-liquid separator so as to remove large solid particles; then, the liquid flows into a sedimentation tank and is subjected to sedimentation for 12 hours, a solid flocculate is removed from the liquid through a round hole filter net, the filtered liquid through the round hole filter net enters a biological reaction tank, pH value is adjusted to 7, 10 grams of compound microbial inoculum are added to each cubic meter of liquid each time, wherein addition of the compound microbial inoculum is conducted once a day and continuously conducted for a week, and finally the materials are allowed to stand for 3 days, and the liquid is discharged. The method of the invention can efficiently remove the pollutants containing ammonia, nitrogen, sulfur, phosphorus and arsenic, and is low in investment cost and has good application prospect.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a method for removing pollutants in sewage. Background technique [0002] With the development of industrial production and the improvement of people's living standards, the volume of industrial sewage and urban domestic sewage is increasing at an alarming rate. One of the reasons for threatening the human living environment. [0003] In order to meet the continuous improvement of the public's requirements for environmental quality, the country has formulated more and more stringent emission standards for nitrogen. Research and development of economical and efficient nitrogen removal treatment technology has become the focus and focus of water pollution control engineering research. Although there are many methods that can effectively remove ammonia, such as physical methods include reverse osmosis, distillation, and soil irrigation; chemical methods...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F9/14C02F3/34C12N1/20C12N1/14C12R1/01C12R1/38C12R1/645C02F101/10C02F101/20C02F101/16
CPCC02F1/001C02F3/34C02F3/348C02F9/00C02F2001/007C02F2101/103C02F2101/16C02F2101/20C12N1/14C12N1/20
Inventor 李娜
Owner 湖北康创科技有限公司
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