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Preparation method of carbon-coated magnetic ferrite modified glucose sensor enzyme electrode

A technology of glucose sensor and magnetic ferrite, which is applied in the field of electrochemical sensing, can solve the problems of difficulty in realizing electron transfer of flavin adenine dinucleotide, inaccessibility to electrode surface, etc., so as to facilitate rapid transfer and reduce distance. , good biocompatibility

Active Publication Date: 2017-06-30
遂宁迪印科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, flavin adenine dinucleotide (FAD), the redox active center of glucose oxidase enzyme (GOx), is embedded in a thick layer of protein and cannot approach the electrode surface, making it difficult to realize flavin adenine dinucleotide Fast electron transfer between FAD and electrode

Method used

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  • Preparation method of carbon-coated magnetic ferrite modified glucose sensor enzyme electrode
  • Preparation method of carbon-coated magnetic ferrite modified glucose sensor enzyme electrode
  • Preparation method of carbon-coated magnetic ferrite modified glucose sensor enzyme electrode

Examples

Experimental program
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Effect test

Embodiment 1

[0034] The method for preparing graphite oxide specifically comprises the steps:

[0035] Accurately weigh 1 gram of graphite powder and 23 mL of concentrated sulfuric acid with a mass fraction of 98%, put the two in the same container, and mix them evenly in an ice-water bath at 4°C; slowly add 0.5 g of sodium nitrate and 3 g of permanganate in sequence Potassium, stirred at room temperature for 30 minutes, then the solution was heated to 35 ° C and continued to stir for 30 minutes, then slowly added deionized water and heated to 98 ° C and stirred for 30 minutes, and finally added 140 mL of deionized water and 30 mL of 30% by mass Hydrogen peroxide solution terminates the reaction; after static treatment, the supernatant is removed to obtain the product, and the above product is subjected to centrifugation (9000rpm), and the product obtained by centrifugation is firstly treated with 1 to 2 times of acid washing, and then washed with water several times. , until the solution ...

Embodiment 2

[0038] A method for preparing a glucose sensor enzyme electrode based on carbon-coated magnetic ferrite modification, comprising the steps of:

[0039] Step A: preparing carbon-coated magnetic ferrite;

[0040] A1: Dissolve ferrous oxalate in an acid solution with pH=4 to prepare a ferrous oxalate acidic solution with a concentration of 0.04M, stir evenly under a nitrogen atmosphere, and add triethylamine (analytically pure, above 99%) dropwise Until the color of the solution no longer deepens, continue to stir until dark green particles are produced in the solution to obtain the first mixed solution;

[0041]A2: Dissolve the graphite oxide prepared in Example 1 in deionized water to obtain a graphite oxide solution with a concentration of 0.06 mg / mL, and ultrasonically treat it in a 100W ultrasonic instrument for 30 minutes; mix the first mixed solution with the graphite oxide solution Equal volumes were mixed to obtain the second mixed solution, and stirred for 12 hours und...

Embodiment 3

[0055] A method for preparing a glucose sensor enzyme electrode based on carbon-coated magnetic ferrite modification, comprising the steps of:

[0056] Step A: preparing carbon-coated magnetic ferrite;

[0057] A1: Dissolve ferrous oxalate in an acid solution with pH=4 to prepare a ferrous oxalate acidic solution with a concentration of 0.08M, stir evenly under a nitrogen atmosphere, and add triethylamine (analytically pure, above 99%) dropwise Until the color of the solution no longer deepens, continue to stir until dark green particles are produced in the solution to obtain the first mixed solution;

[0058] A2: Dissolve commercially available graphite oxide in deionized water to prepare a graphite oxide solution with a concentration of 0.1 mg / mL, and ultrasonically treat it in a 100W ultrasonic instrument for 30 minutes; mix the first mixed solution with the graphite oxide solution in equal volumes The second mixed solution was prepared, stirred for 12 hours under an inert...

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Abstract

The invention discloses a preparation method of a carbon-coated magnetic ferrite modified glucose sensor enzyme electrode and relates to the field of electrochemical sensing technology. The invention provides a novel method for preparing a carbon-coated magnetic ferrite. The method is applied in the manufacturing of a glucose sensor enzyme electrode. Based on the characteristics of large specific surface area and good biocompatibility of the carbon-coated magnetic ferrite, the adoption of the carbon-coated magnetic ferrite modified enzyme electrode is beneficial to fixation of glucose oxidase and increase of load capacity of the electrode's surface enzyme. Meanwhile, as magnetic functional graphite is adopted and particles on the surface of the graphite have characteristics of small particle size, good conductivity and strong adsorption capacity, the distance between the active centre of glucose oxidase and the electrode is shortened, which is good for rapid electron transfer. The glucose sensor enzyme electrode provided by the invention has advantages of stable performance and high sensitivity, and can be widely applied in fields of clinic, medicine and food industry, etc.

Description

technical field [0001] The invention belongs to the technical field of electrochemical sensing, in particular to a method for preparing a glucose sensor enzyme electrode based on carbon-coated magnetic ferrite modification. Background technique [0002] Glucose is the main component of carbohydrates in animals and plants, and its quantitative determination plays an important role in biochemistry, clinical chemistry and food analysis. Glucose sensors are the most studied biosensors in the field of biosensors. At present, the enzyme electrode glucose sensor based on glucose oxidase is attracting more and more attention. [0003] Glucose oxidase (GOx) is a polymer substance that can specifically catalyze β-D-glucose. Taking advantage of its high catalytic activity and biomolecular recognition characteristics, GOx is often selected as the preferred active substance in glucose sensors. The specific mechanism is: glucose oxidase (GOx) oxidizes glucose to gluconic acid, and relea...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3271G01N27/48
Inventor 王焱刘新宇冯哲圣陈金菊
Owner 遂宁迪印科技有限公司
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