Preparation method of probiotics microcapsule

A technology of microcapsules and probiotics, applied in the field of microorganisms, can solve the problems that probiotic strains cannot be quickly colonized and released, cannot protect the cells, and the microcapsules have poor acid resistance, so as to improve physical stability, rapidly multiply probiotics, reduce effect of sensitivity

Active Publication Date: 2017-08-01
宝健(北京)生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current probiotic products on the market mainly have the following problems: 1. Without embedding, the protective agent is directly added, and then freeze-dried into powder and packaged. Once the probiotics are released in the stomach, they are easily damaged by gastric acid and bile salts; 2. Common single wall materials are mostly used in embedding, and compound wall materials are rarely used. There are problems such as hig

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  • Preparation method of probiotics microcapsule
  • Preparation method of probiotics microcapsule
  • Preparation method of probiotics microcapsule

Examples

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Embodiment 1

[0034] The preparation of embodiment 1 bifidobacterium lactis microcapsules

[0035] A preparation process of Bifidobacterium lactis microcapsules, the strains are purchased from the China Industrial Microbiology Strain Preservation Management Center, including the following specific steps:

[0036] (1) Compound freeze-drying protective agent preparation: 0.2% glycerin, 0.5% glycine, 2% lactose, 0.5% sorbitol, 2% bovine serum albumin, constant volume with distilled water, sterilized at 115°C for 20min before use, Use after cooling.

[0037] (2) Preparation of embedding wall material: premix methylcellulose (relative molecular mass: 40kDa) with galacto-oligosaccharide according to the mass ratio of 1:2, then dissolve it in sterile water, and mix it evenly. The Abbe refractometer measures the refractive index, and the refractive index is finally controlled at 1.35. Stir for 15 min with a magnetic stirrer. Put the mixed solution into a radiation-resistant PE plastic bag for se...

Embodiment 2

[0043] The preparation of embodiment 2 Lactobacillus acidophilus microcapsules

[0044] A preparation process of Lactobacillus acidophilus microcapsules, the strains are purchased from the China Industrial Microbiology Strain Preservation Management Center, including the following specific steps:

[0045] (1) Compound freeze-drying protective agent preparation: 0.15% glycerin, 0.2% glycine, 1% lactose, 1% sorbitol, 1.5% bovine serum albumin, constant volume with distilled water, sterilized at 121°C for 15 minutes before use, Use after cooling.

[0046] (2) Preparation of embedding wall material: premix methylcellulose (relative molecular mass: 50kDa) with galacto-oligosaccharide according to the mass ratio of 1:8, then dissolve it in sterile water and mix it evenly. The Abbe refractometer measures the refractive index, and the refractive index is finally controlled at about 1.40. Finally, a magnetic stirrer was used to stir for 20 min. Put the mixed solution into a radiatio...

Embodiment 3

[0051] The preparation of embodiment 3 Bifidobacterium longum microcapsules

[0052] A preparation process of Bifidobacterium longum microcapsules, the bacterial strains are purchased from the China Industrial Microorganism Strain Preservation Management Center, including the following specific steps:

[0053] (1) Compound freeze-drying protective agent preparation: 0.25% glycerin, 0.3% glycine, 1% lactose, 1.5% sorbitol, 2% bovine serum albumin, constant volume with distilled water, sterilized at 118°C for 18 minutes before use, Use after cooling.

[0054] (2) Preparation of embedding wall material: premix methylcellulose (relative molecular mass: 60kDa) with galacto-oligosaccharide according to the mass ratio of 1:6, then dissolve it in sterile water, and mix it evenly. The Abbe refractometer measures the refractive index, and the refractive index is finally controlled at about 1.44. Finally, a magnetic stirrer was used to stir for 20 min. Put the mixed solution into a ra...

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Abstract

The invention discloses a preparation method of a probiotics microcapsule, comprising the steps of culturing and activating probiotics strains; centrifugally collecting thalluses, adding compound cryprotectants in the thalluses; embedding: preparing a bacteria solution, performing embedding, mixing the bacteria solution, an embedding wall material solution and a sodium alginate solution, stirring, dripping the mixed bacteria solution into a calcium chloride solution, solidifying, washing with sterile water and filtering; performing freeze drying on an obtained microcapsule, thus obtaining the probiotics microcapsule. A methylcellulose-galactooligosaccharide graft copolymer is used as an embedding wall material, so that the stability of a probiotics preparation in a storage and transportation process is enhanced, the damage of gastric acid, bile salt and the like to the thalluses is effectively reduced; the probiotics microcapsule has relatively strong acid-resisting and bile salt-resisting capabilities, the quantity and activity of the probiotics strains in small intestine is greatly improved, the regulation and improvement of intestinal flora are facilitated; the method is suitable for industrial production, and the range of application of methylcellulose is widened at the same time; important economic significance is realized.

Description

technical field [0001] The invention relates to the technical field of microorganisms. More specifically, it relates to a preparation method of probiotic microcapsules. Background technique [0002] Probiotics are a class of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize the human intestinal tract and reproductive system and can produce definite health effects, thereby improving the host's micro-ecological balance and exerting beneficial effects. In recent years, more and more studies have confirmed that probiotics can inhibit the growth of pathogenic bacteria, eliminate carcinogenic factors, maintain normal intestinal flora, and have important physiological effects such as alleviating lactose intolerance, lowering cholesterol, and improving immunity. [0003] Bifidobacteria and Lactobacillus are important beneficial bacteria in the human intestinal tract, and are often used as important sign...

Claims

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Application Information

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IPC IPC(8): A23L33/135A23L33/16A23L33/125A23P10/30
CPCA23V2002/00A23V2300/12A23V2200/3204
Inventor 张合亮袁立军刘颖
Owner 宝健(北京)生物技术有限公司
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