Preparation method of probiotics microcapsule

Abstract

The invention discloses a preparation method of a probiotics microcapsule, comprising the steps of culturing and activating probiotics strains; centrifugally collecting thalluses, adding compound cryprotectants in the thalluses; embedding: preparing a bacteria solution, performing embedding, mixing the bacteria solution, an embedding wall material solution and a sodium alginate solution, stirring, dripping the mixed bacteria solution into a calcium chloride solution, solidifying, washing with sterile water and filtering; performing freeze drying on an obtained microcapsule, thus obtaining the probiotics microcapsule. A methylcellulose-galactooligosaccharide graft copolymer is used as an embedding wall material, so that the stability of a probiotics preparation in a storage and transportation process is enhanced, the damage of gastric acid, bile salt and the like to the thalluses is effectively reduced; the probiotics microcapsule has relatively strong acid-resisting and bile salt-resisting capabilities, the quantity and activity of the probiotics strains in small intestine is greatly improved, the regulation and improvement of intestinal flora are facilitated; the method is suitable for industrial production, and the range of application of methylcellulose is widened at the same time; important economic significance is realized.

Description

technical field [0001] The invention relates to the technical field of microorganisms. More specifically, it relates to a preparation method of probiotic microcapsules. Background technique [0002] Probiotics are a class of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize the human intestinal tract and reproductive system and can produce definite health effects, thereby improving the host's micro-ecological balance and exerting beneficial effects. In recent years, more and more studies have confirmed that probiotics can inhibit the growth of pathogenic bacteria, eliminate carcinogenic factors, maintain normal intestinal flora, and have important physiological effects such as alleviating lactose intolerance, lowering cholesterol, and improving immunity. [0003] Bifidobacteria and Lactobacillus are important beneficial bacteria in the human intestinal tract, and are often used as important sign...

AI Technical Summary

Problems solved by technology

The current probiotic products on the market mainly have the following problems: 1. Without embedding, the protective agent is directly added, and then freeze-dried into powder and packaged. Once the probiotics are released in the stomach, they are easily damaged by gastric acid and bile salts; 2. Common single wall materials are mostly used in embedding, and compound wall materials are rarely used. There are problems such as high bacterial mortality and poor acid resistance of microcapsules.

[0009] The traditional single-layer embedding not only has low yield but also poor embedding effect, and cannot protect the bacteria well; although the three-layer and above microcapsule embedding technology improves the stability of the bacteria, the process is cumbersome. Time-consuming, the probiotic strains in the intestine cannot be quickly colonized and released

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