A kind of heparinized bacterial nanocellulose/chitosan composite tube and its preparation method and application

Abstract

The invention relates to a heparinized bacterial nanocellulose / chitosan composite tube and a preparation method and application thereof. The heparinized bacterial nanocellulose / chitosan composite tube has a 3D fiber network structure; chitosan is deposited on the surface of a nanofiber and in a network; and heparin is grafted on bacterial nanocellulose and the chitosan. The preparation method comprises the steps of putting the pretreated bacterial nanocellulose BNC into a chitosan CH acid solution for oscillation, immersing the bacterial nanocellulose BNC into an alkaline solution for standing and cleaning to obtain the BNC / CH composite tube; and putting the BNC / CH composite tube into a buffer solution for oscillation and putting the composite tube into a heparin Hep cross-linking system for oscillating and cleaning to obtain a BNC / CH-Hep composite tube. The mechanical property is strengthened, the heparinized bacterial nanocellulose / chitosan composite tube has a better anticoagulation effect, thrombosis is prevented, adhesion, multiplication and growth of cells are facilitated, and meanwhile, the chitosan is capable of achieving slow release of the heparin through degradation in a body and maintaining higher vascular patency rate, and has great potential in application in the field of tissue engineering, such as an artificial blood vessel, an artificial trachea, a nerve conduit and a catheter.

Description

technical field

[0001] The invention belongs to the technical field of bionano materials, and in particular relates to a heparinized bacterial nanocellulose / chitosan composite tube and a preparation method and application thereof. Background technique

[0002] Bacterial nanocellulose (Bacterial nono-cellulose, BNC) is a polysaccharide polymer secreted extracellularly by microorganisms (mainly bacteria of the genus Acetobacter, such as Gluconacetobacter xylinum). Its chemical composition is the same as that of plant cellulose, and it is a glucan polymer formed by connecting glucopyranose monomers with β-1,4 glycosidic bonds, but its properties are different from those of plant cellulose, including high chemical purity (without mixing Miscellaneous lignin, hemicellulose and other polysaccharides), with nanofiber network structure, high crystallinity, good biocompatibility, etc. Due to its unique properties, it has attracted worldwide attention, and it has entered the practica...

Images