A kind of heparinized bacterial nanocellulose/chitosan composite tube and its preparation method and application

A technology of nanocellulose and chitosan, applied in pharmaceutical formulations, transportation and packaging, packaged food, etc., can solve the problem of decreased anticoagulant activity of heparin, it is difficult to achieve long-term maintenance of heparinization effect, and it is difficult to work for a long time, etc. problem, to achieve the effect of enhanced anticoagulant activity, enhanced anticoagulant activity, and favorable adhesion

Active Publication Date: 2020-11-13
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physically adsorbed heparin has high anticoagulant activity, but it is easy to burst and release, and it is difficult to achieve long-term maintenance of heparinization effect; chemical cross-linking method, especially covalently bound heparin, has high stability, but the compounded heparin anticoagulant The blood activity has decreased. Therefore, when the material is heparinized, it is necessary to comprehensively consider the firmness of heparin immobilization, the concentration of heparin on the surface of the material, and whether to maintain the biological activity of heparin, in order to achieve the best results.
[0005] At present, there are mainly two methods for bacterial nanocellulose compounding heparin. The first method is to directly soak BNC with heparin solution, and then dry to remove water. Released over time, it is difficult to work long-term

Method used

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  • A kind of heparinized bacterial nanocellulose/chitosan composite tube and its preparation method and application
  • A kind of heparinized bacterial nanocellulose/chitosan composite tube and its preparation method and application
  • A kind of heparinized bacterial nanocellulose/chitosan composite tube and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] (1) Obtain a BNC tube with an inner diameter of 3mm, an outer diameter of 8mm, and a length of 50mm by in-situ static culture, place it in 1% NaOH solution, 80°C, water bath for 4h, and then replace it repeatedly with ultrapure water to Neutrals are obtained from preconditioned BNC tubing.

[0050] (2) 100 mL of 1% acetic acid solution was prepared with ultrapure water, and 0.1 g of chitosan was dissolved in the acetic acid solution. Place the BNC tube sleeve obtained in step (1) on a silicone tube with an outer diameter of 3mm in the acetic acid solution of chitosan, 30°C, 100rpm, shake in a water bath for 12h, then immerse in 1% NaOH solution and let stand for 4h. Then it was washed and replaced repeatedly to neutrality with ultrapure water to obtain the bacterial nanocellulose / 0.1% chitosan BNC / 0.1% CH composite tube.

[0051] (3) Place the BNC / 0.1% CH composite tube obtained in step (2) in 0.05M MES buffer solution with pH=5.5, shake in a water bath at 30° C. at 10...

Embodiment 2

[0054] (1) Through in-situ static culture, the original BNC tube with an inner diameter of 10 mm, an outer diameter of 15 mm and a length of 200 mm (the schematic diagram of the in-situ static culture bioreactor is shown in Figure 10 shown), placed in 1% NaOH solution, 80° C., in a water bath for 4 hours, and then repeatedly replaced to neutral with ultrapure water to obtain a pretreated BNC tube.

[0055] (2) 100 mL of 1% citric acid solution was prepared with ultrapure water, and 1 g of chitosan was dissolved in the above citric acid solution. Place the BNC tube sleeve obtained in step (1) on a silicone tube with an outer diameter of 10mm in the citric acid solution of chitosan, 30°C, 100rpm, shake in a water bath for 12h, then immerse in 1% Na 2 CO 3 The solution was left to stand for 4 hours, then washed, and replaced repeatedly to neutrality with ultrapure water to obtain a bacterial nanocellulose / 1% chitosan BNC / 1% CH composite tube.

[0056] (3) Place the BNC / 1%CH co...

Embodiment 3

[0059] (1) After the bacterial nanocellulose membrane obtained by in-situ static culture is fully pulverized, the BNC homogenate with a cellulose dry weight content of 0.5% is obtained and poured into a glass rod (3mm in diameter) and a polypropylene tube (with an inner diameter of 8mm, thickness 1mm) in the cavity between the two ends are fixed with rubber stoppers (the schematic diagram of the mold is shown in Figure 11 shown). After repeated freezing and thawing at -20°C and 30°C for 5 times, freeze at -80°C for 4 hours, open the rubber stoppers at both ends of the mold, and freeze-dry to obtain a pretreated BNC tube.

[0060] (2) 100 mL of 1% hydrochloric acid solution was prepared with ultrapure water, and 5 g of chitosan was dissolved in the hydrochloric acid solution. Place the BNC tube sleeve obtained in step (1) on a silicone tube with an outer diameter of 3mm in the hydrochloric acid solution of chitosan, 30°C, 100rpm, shake in a water bath for 12h, then immerse in...

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Abstract

The invention relates to a heparinized bacterial nanocellulose / chitosan composite tube and a preparation method and application thereof. The heparinized bacterial nanocellulose / chitosan composite tube has a 3D fiber network structure; chitosan is deposited on the surface of a nanofiber and in a network; and heparin is grafted on bacterial nanocellulose and the chitosan. The preparation method comprises the steps of putting the pretreated bacterial nanocellulose BNC into a chitosan CH acid solution for oscillation, immersing the bacterial nanocellulose BNC into an alkaline solution for standing and cleaning to obtain the BNC / CH composite tube; and putting the BNC / CH composite tube into a buffer solution for oscillation and putting the composite tube into a heparin Hep cross-linking system for oscillating and cleaning to obtain a BNC / CH-Hep composite tube. The mechanical property is strengthened, the heparinized bacterial nanocellulose / chitosan composite tube has a better anticoagulation effect, thrombosis is prevented, adhesion, multiplication and growth of cells are facilitated, and meanwhile, the chitosan is capable of achieving slow release of the heparin through degradation in a body and maintaining higher vascular patency rate, and has great potential in application in the field of tissue engineering, such as an artificial blood vessel, an artificial trachea, a nerve conduit and a catheter.

Description

technical field [0001] The invention belongs to the technical field of bionano materials, and in particular relates to a heparinized bacterial nanocellulose / chitosan composite tube and a preparation method and application thereof. Background technique [0002] Bacterial nanocellulose (Bacterial nono-cellulose, BNC) is a polysaccharide polymer secreted extracellularly by microorganisms (mainly bacteria of the genus Acetobacter, such as Gluconacetobacter xylinum). Its chemical composition is the same as that of plant cellulose, and it is a glucan polymer formed by connecting glucopyranose monomers with β-1,4 glycosidic bonds, but its properties are different from those of plant cellulose, including high chemical purity (without mixing Miscellaneous lignin, hemicellulose and other polysaccharides), with nanofiber network structure, high crystallinity, good biocompatibility, etc. Due to its unique properties, it has attracted worldwide attention, and it has entered the practica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/34A61L27/50A61L27/58A61L33/10
CPCA61L27/20A61L27/34A61L27/50A61L27/507A61L27/58A61L33/0011A61L2400/18A61L2430/22A61L2430/32C08L1/02C08L5/08
Inventor 洪枫包露涵李雪唐敬玉陈琳
Owner DONGHUA UNIV
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