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Fibers for tissue engineering bone repairing, tissue engineering bone repairing scaffold and preparation method thereof

A tissue-engineered bone and fiber technology, applied in medical science, prosthesis, etc., can solve the problems of high melting temperature, biochemical damage, enhanced physical damage, etc., achieve good mechanical properties, excellent and rapid osteogenesis and angiogenesis, The effect of maintaining cell viability

Active Publication Date: 2018-01-09
MEDPRIN REGENERATIVE MEDICAL TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, increasing the polymer concentration will increase the physical damage it causes to the cell, thus affecting subsequent cell function
The strengthening of cross-linking strength will affect the printing process itself. Enhancing cross-linking before printing will easily lead to failure of the extrusion process. Post-printing cross-linking post-treatment will prolong the operation time. At the same time, no matter what kind of cross-linking will reduce the activity and function of cells.
For the existing technology of cross-printing thermoplastic polymers and hydrogels, although the mechanical strength of the scaffold can be improved, the thermoplastic polymers require a higher melting temperature during printing, and will affect the adjacent hydrogels during the molding process. Cells cause biochemical damage, such as damage to membrane proteins or DNA, resulting in decreased cell viability, and the survival rate of cells encapsulated in thermoplastic polymers is also low

Method used

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  • Fibers for tissue engineering bone repairing, tissue engineering bone repairing scaffold and preparation method thereof
  • Fibers for tissue engineering bone repairing, tissue engineering bone repairing scaffold and preparation method thereof
  • Fibers for tissue engineering bone repairing, tissue engineering bone repairing scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] In this example, agarose with a low gelation temperature is used as the main component of the hydrogel preparation material, and fibrin is used as an auxiliary component of the hydrogel preparation material. The powder material is self-made 10-50 μm bioglass powder, and the growth factor is selected. Platelet-derived growth factor (PDGF), the printed cells are bone marrow mesenchymal stem cells. The specific implementation steps are as follows:

[0050] (1) The 58S bioglass was prepared by the sol-gel method, that is, a certain amount of tetraethyl orthosilicate, triethyl phosphate, and calcium nitrate were dissolved in deionized water, and after standing at room temperature for 24 hours, the glass gel was taken out and Vacuum dried, after drying at 650 o Heat treatment at C for 5 hours, ball milling, sieving, and irradiation sterilization to obtain sterile biological glass powder with the required particle size.

[0051] (2) Put sterile agarose at 90 o Dissolve in n...

Embodiment 2

[0056] In this example, sodium alginate is used as the main component of the hydrogel preparation material, and hyaluronic acid is used as the auxiliary component of the hydrogel preparation material. The powder material is self-made polycaprolactone microspheres of 20-100 μm, and the growth factor is bone morphology. BMP-2, the printed cells are osteoblasts. The specific implementation steps are as follows:

[0057] (1) Polycaprolactone microspheres were prepared by solvent evaporation method. That is, polycaprolactone with a molecular weight of 50000 is dissolved in dichloromethane, and the concentration of the prepared solution is 5 wt%. Dissolve 1799-type polyethylene glycol (PVA) in deionized water under heating conditions to obtain a PVA solution with a concentration of 1 wt%. Under the condition of stirring at a stirring rate of 800 rpm, the polycaprolactone solution was slowly added into the PVA solution with a needle, wherein the volume ratio of the polycaprolactone...

Embodiment 3

[0063] In this example, gelatin is used as the main component of the hydrogel preparation material, and polypeptide is the auxiliary component of the hydrogel preparation material. The powder material is selected from commercially available nano-hydroxyapatite powder of 20-100 nm, and the growth factor is selected from fibroblast growth. Factor (FGF), the printed cells are selected from adipose stem cells. The specific implementation steps are as follows:

[0064] (1) Nano-hydroxyapatite was purchased from Sigma in the United States and sterilized by irradiation before use. Mix sterile gelatin and peptide at 37 o Dissolve in sterile phosphate buffer at C to make the first sol of 15wt% gelatin+5wt% polypeptide and the second sol of 10wt% gelatin+5wt% polypeptide. Add nano-hydroxyapatite powder into the first sol and mix evenly, the powder addition amount is 20wt%, to obtain sol-powder composite viscous slurry.

[0065] (2) Add a certain amount of transglutaminase and fibrobl...

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Abstract

The invention provides fibers for tissue engineering bone repairing, a tissue engineering bone repairing scaffold and a preparation method thereof. The fibers for the tissue engineering bone repairing, provided by the invention, have a double-layered structure comprising an inner layer and an outer layer; the inner layer provides suitable mechanical strength for the whole fibers, and the outer layer can effectively maintain the activity and functionality of cells; the fibers for the tissue engineering bone repairing are applied to preparation of the tissue engineering bone repairing scaffold;the prepared tissue engineering bone repairing scaffold can be used for keeping the activity of the cells very well, has excellent rapid bone formation and blood vessel formation functions and has good mechanical properties.

Description

technical field [0001] The invention relates to the field of tissue engineering bone repair, and more specifically, to a fiber for tissue engineering bone repair, a scaffold for tissue engineering bone repair and a preparation method thereof. Background technique [0002] Bone tissue has the function of self-repair and regeneration and reconstruction. Currently, large-area bone defects caused by trauma, tumor, infection, and osteoporosis caused by aging population cannot be healed only by the self-repair ability of bone. Bone graft filling surgery is the most effective means of treating such bone defects. Autologous bone repair is the gold standard for bone defect repair, but its source is limited; allogeneic bone or xenograft bone has certain limitations in clinical use due to problems such as difficulty in shaping, immune rejection, disease transmission, and ethics; routinely used inorganic Material artificial bone (such as calcium phosphate, etc.) has a wide range of sou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/22A61L27/20A61L27/10A61L27/12A61L27/18A61L27/50A61L27/52A61L27/54
Inventor 张婧邓坤学袁玉宇
Owner MEDPRIN REGENERATIVE MEDICAL TECH
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