A kind of culturing system and culturing method of aerobic anaerobic bacteria using syngas fermentation to produce alcohol

A culture system and synthesis gas technology, applied in the field of microbial fermentation, can solve the problems of weak product synthesis ability and low alcohol production, and achieve the effect of improving the highest concentration and the ability of fermentation to produce alcohol

Active Publication Date: 2021-08-24
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the purpose of the present invention is to provide a kind of method that aerobic anaerobic bacterium utilizes the method of synthesis gas fermentation alcohol production ability, to solve the problem of weak product synthesis ability and low alcohol yield in the prior art using synthesis gas fermentation process question

Method used

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  • A kind of culturing system and culturing method of aerobic anaerobic bacteria using syngas fermentation to produce alcohol
  • A kind of culturing system and culturing method of aerobic anaerobic bacteria using syngas fermentation to produce alcohol
  • A kind of culturing system and culturing method of aerobic anaerobic bacteria using syngas fermentation to produce alcohol

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The macroelement mother liquor of 15ml, the reducing agent of 482.5ml nutrient solution and 2.5ml are formulated into 500ml culture medium, wherein macroelement mother liquor comprises the component of following mass content: 8% sodium chloride, 10% ammonium chloride, 1% chlorine Potassium chloride, 1% potassium dihydrogen phosphate, 2% magnesium sulfate, 0.4% calcium chloride and the rest of the water; the nutrient aqueous solution includes the components of the following quality: the yeast extract of 0.5g, the morpholine of 2.5g Ethansulfonic acid and the water of remainder; water.

[0059]Distribute the prepared culture medium into 100mL serum bottles with a liquid volume of 30mL. Seal the mouth of the bottle with a rubber stopper, fill the serum bottle with 99.999% nitrogen gas at a rate of 2.5 L / min with a nitrogen bottle connected to a needle to remove residual oxygen in the serum bottle, and stop the inflation after the inflation time reaches 5 minutes. Sterili...

Embodiment 2

[0062] The macroelement mother liquor of 10ml, the nutrient aqueous solution of 487.5ml and the reducing agent of 2.5ml are formulated into 500ml culture medium, and wherein macroelement mother liquor comprises the component of following mass content: 8% sodium chloride, 10% ammonium chloride, 1% chlorine Potassium chloride, 1% potassium dihydrogen phosphate, 2% magnesium sulfate, 0.4% calcium chloride and the rest of the water; the nutrient aqueous solution includes the components of the following quality: the yeast extract of 0.5g, the morpholine of 2.5g Ethansulfonic acid and the water of remainder; water.

[0063] Distribute the prepared culture medium into 100mL serum bottles with a liquid volume of 30mL. Seal the mouth of the bottle with a rubber stopper, fill the serum bottle with 99.999% nitrogen gas at a rate of 2.5 L / min with a nitrogen bottle connected to a needle to remove residual oxygen in the serum bottle, and stop the inflation after the inflation time reaches...

Embodiment 3

[0066] The macroelement mother liquor of 15ml, 484ml nutrient aqueous solution and the reductant of 1.0ml are formulated into 500ml culture medium, and wherein macroelement mother liquor comprises the component of following mass content: 8% sodium chloride, 10% ammonium chloride, 1% chloride Potassium, 1% potassium dihydrogen phosphate, 2% magnesium sulfate, 0.4% calcium chloride and the water of remainder; Nutrient aqueous solution comprises the component of following quality: the yeast extract of 0.5g, the morpholine B of 2.5g Sulfonic acid and the water of balance; Reducing agent comprises the component of following mass content: the sodium hydroxide of 0.9%, the L-cysteine ​​hydrochloride of 4%, the sodium sulfide nonahydrate of 4% and the water of balance .

[0067] Distribute the prepared culture medium into 100mL serum bottles with a filling volume of 20mL. Seal the mouth of the bottle with a rubber stopper, fill the serum bottle with 99.999% nitrogen gas at a rate of ...

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Abstract

The invention provides a culture system and a culture method for aerobic anaerobic bacteria to ferment with syngas. The culture system includes the following components: trace elements, zinc ions, molybdate ions and culture medium; the zinc ions are in The concentration in the culture system is 100-500 μM; the present invention uses the culture system to ferment the aerobic anaerobic bacteria, which improves the ability of the aerobic anaerobic bacteria to ferment and produce alcohols. Taking Clostridium carboxidivorans P7 as an example, the cells of the strain Concentration up to OD 600 nm~1.543Abs, the highest yield of ethanol was 2.531g / L, the highest yield of n-butanol was 0.318g / L, and the content of n-hexanol was 0.034g / L.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a culture system and a culture method for aerobic anaerobic bacteria to ferment and produce alcohol by syngas. Background technique [0002] Syngas is a gas that mainly contains CO, H 2 and a small amount of CO 2 Syngas comes from a wide range of sources, such as coal, oil, natural gas, industrial waste gas (such as coke oven gas, refinery gas), municipal solid waste, and biomass. At present, the excessive use of fossil energy and the burning of urban waste and straw have caused CO, CO 2 、CH 4 The rising content of gases such as CO2 damages human health, causes the global greenhouse effect, exacerbates the El Niño phenomenon, and leads to many serious social, environmental and economic problems such as climate anomalies, sea level rise, and crop yield decline. The above syngas is generally treated by means of absorption or combustion, which has low treatment ef...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/06C12P7/16C12P7/04C12N1/20C12R1/145
CPCC12N1/20C12P7/04C12P7/065C12P7/16Y02E50/10
Inventor 黄志勇谢彬涛吴冠勋郭亚琼韩一凡
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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