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Microbial population sensing signal molecule degrading bacterium and application thereof in disease prevention and control

A quorum sensing signal and microbial technology, applied in the direction of microorganism-based methods, microorganisms, microorganisms, etc., can solve the problems of acyl side chain length, saturation difference, etc., and achieve the effect of reducing pesticide abuse

Active Publication Date: 2018-04-10
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

AHLs are quorum sensing signals unique to Gram-negative bacteria. Most of them have the same homoserine lactone ring structure and acyl side chains, but there are differences in the length and degree of saturation of the acyl side chains.

Method used

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  • Microbial population sensing signal molecule degrading bacterium and application thereof in disease prevention and control
  • Microbial population sensing signal molecule degrading bacterium and application thereof in disease prevention and control
  • Microbial population sensing signal molecule degrading bacterium and application thereof in disease prevention and control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Acquisition and Identification of Nitroreductor Pseudomonas Strain W-7

[0045] 1. Isolation and screening of strain W-7

[0046] (1) Soil sample collection: Sewer sewage samples collected from Guangzhou were used as microbial sources

[0047] Soil samples were collected from the sewer near South China Agricultural University in Guangzhou City, Guangdong Province on October 9, 2014. Sampling, bagging, and preservation were carried out as microbial sources and brought back to the school for strain isolation.

[0048] (2) Enrichment culture of bacterial strains: Prepare MSM medium, put 50mL of basal medium into a 250mL Erlenmeyer flask for sterilization, add AHLs mother liquor (methanol as solvent) under aseptic conditions after cooling, to make the final concentration of AHLs At the same time, 5 g of soil samples were added and cultured on a shaker at 30°C and 200 rpm for 7 days, and then transferred to the second batch of MSM medium containing 10 μM AHLs at a...

Embodiment 2

[0071] Antibiotic susceptibility analysis of embodiment 2 bacterial strain W-7

[0072] In order to better study the biocontrol potential of the bacterial strain W-7 obtained in Example 1, we conducted in-depth research on the biological characteristics of the bacterial strain W-7. The sensitivity of strain W-7 to different antibiotics was experimentally studied, as Figure 5 shown.

[0073] The experimental results show that the strain W-7 has a resistance of more than 400 μg / mL to ampicillin, a resistance of 200 μg / mL to gentamicin, streptomycin and chloramphenicol, and a resistance of 10 μg to tetracycline. / mL. This result is helpful for selecting appropriate antibiotics as a reference in follow-up studies.

Embodiment 3

[0074] Degradability determination of embodiment 3 bacterial strain W-7 to OdDHL

[0075] 1. Strain cultivation and sample collection: Streak the strain W-7 on the LB solid medium plate and cultivate overnight at 30°C. Pick a single colony and culture overnight with LB liquid medium to obtain a bacterial liquid. Take 1 OD 600 Inoculate 1 mL of MSM inorganic salt medium with OdDHL as the sole carbon source, and the final concentration of OdDHL in MSM inorganic salt medium is 80 μM. The reaction mixture was placed in a 2 mL centrifuge tube, and the centrifuge tube was incubated at 30° C. and 200 rpm for 24 h. After 24 hours, take 5 μL of the reaction mixture and spot the sample on the top of the agar strip, and then spot the bacterial solution of the reporter strain (Agrobacterium tumefaciens NT1) below. Then place the culture dish with the reaction mixture and the reporter strain in a 28°C incubator, and observe the experimental results after culturing for 24 hours. Wherein...

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Abstract

The invention discloses a microbial population sensing signal molecule degrading bacterium and an application thereof in disease prevention and control. The degrading bacterium is a pseudomonas nitroreducens strain W-7, wherein the strain is preserved in China Center for Type Culture Collection on November 2, 2017 with preservation number of CCTCC NO: M2017649. The strain is stable in activity; the strain can grow by taking such population sensing signal molecules AHLs as OdDHL and the like as a unique carbon source, and the strain can take an obvious and rapid degrading effect on the signal molecules; meanwhile, the strain takes a relatively good degrading effect on other population sensing signal molecules such as OHHL and DSF; furthermore, the strain also has the characteristics of being simple in culture method, high in growth speed, not easy for variation and strong in adaptive capacity; and the strain has a huge promotion and application potential in such aspects as population quenching direction, inhibiting soft rot disease and preventing and controlling microbial population sensing signal mediated pathological disease harm. With the application of the degrading bacterium provided by the invention, the problem of abuse of pesticides can be relieved, and meanwhile, a new thinking can be prevented from biological prevention and control of plant diseases.

Description

technical field [0001] The invention belongs to the technical field of biological control. More specifically, it relates to a microbial quorum sensing signal molecule degrading bacterium and its application in disease control. Background technique [0002] The effective control of plant pathogenic bacteria has always been an important problem in agricultural production. For example, Erwinia sp. and Dickeya sp. are Gram-negative plant pathogenic bacteria. Among them, the Dicardia genus is a new genus of plant pathogenic bacteria established in recent years, and its model bacteria is the original Erwiniachrysanthemi. Dickeya sp. can cause major diseases of many important food crops and economic crops, such as rice bacterial foot rot, banana bacterial soft rot, potato bacterial soft rot, etc., leading to related The reduction in crop production has caused significant economic losses. Soft rot is a kind of important agricultural disease, and there is no very effective control...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P1/00C12R1/38
CPCA01N63/00A01N63/10C12N1/205C12R2001/38
Inventor 陈少华范兴辉张炼辉王惠杉叶田阳芳李绮婷
Owner SOUTH CHINA AGRI UNIV
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