A compound microbial drink for hangover and liver protection and preparation method thereof

A technology of compound microorganisms, anti-alcoholic and liver-protecting, which is applied in the field of anti-alcoholic and liver-protecting compound microbial beverages and its preparation. Effects of damage and improvement of nutritional value

Active Publication Date: 2021-01-05
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also some health drinks for hangover and liver protection on the market, but the effect is very slow. The effects of hangover, liver protection and liver protection are not obvious. There is no ideal hangover and liver protection drink on the market.

Method used

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  • A compound microbial drink for hangover and liver protection and preparation method thereof
  • A compound microbial drink for hangover and liver protection and preparation method thereof
  • A compound microbial drink for hangover and liver protection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Anti-alcoholic strain flat plate initial screening

[0036] Plate screening medium: potassium dihydrogen phosphate 1g / L, dipotassium hydrogen phosphate 0.5 g / L, magnesium sulfate 0.5g / L, sodium chloride 5g / L, ferric chloride 0.01g / L, ammonium sulfate 5g / L, Absolute ethanol 5% (v / v), agar 20g / L, pH7.2;

[0037] Spore fermentation medium: corn yellow powder 40 g / L, soybean protein isolate powder 40 g / L, honey 40 g / L, sodium chloride 5 g / L, potassium dihydrogen phosphate 1 g / L, dipotassium hydrogen phosphate 0.5 g / L L, magnesium sulfate 0.5g / L, pH7.2;

[0038] More than 100 strains of bacillus stored in the laboratory of our company were screened on the plate, and it was found that the bacillus could grow rapidly on the plate with JCD-H-16, JCD-Q-8, JCD-B-17, JCD-L-2 JCD-L-15: And carried out the ethanol retention rate test, the results are shown in Table 1 and found that JCD-H-16 has obvious advantages in the ethanol retention rate, so it was initially selecte...

Embodiment 2

[0041] The screening test of the ethanol retention rate of embodiment 2 compound microorganism fermented liquid

[0042] The ethanol retention rate refers to the ability of anti-alcohol substances to reduce the amount of ethanol added to it through encapsulation, or to inhibit or delay the absorption of ethanol in the body.

[0043] The formula for measuring the concentration of ethanol in a solution is as follows:

[0044] Ethanol retention rate=[(A-B) / A]×100%

[0045] Among them: A: the amount of ethanol added

[0046] B: Ethanol content after treatment

[0047] Test method: After fermenting the existing various fermentation bacteria liquids separately, compound them according to a certain proportion, and then measure their ethanol retention rate, and divide them into the following 11 groups in total:

[0048] A: Bacillus subtilis fermentation broth; B: Sulfur bacteria fermentation broth; C: Rhizopus fermentation broth; D: Saccharomyces fermentation broth; E: Lactobacillu...

Embodiment 3

[0052] Embodiment 3 A compound microbial drink for relieving alcohol and protecting the liver. The compound microbial drink is mixed with 30-40 parts of Bacillus subtilis fermentation liquid, 30-40 parts of Thiobacillus permanthus fermentation liquid, and 30-40 parts of Rhizopus oryzae fermentation liquid made;

[0053] Its specific preparation method comprises the following steps:

[0054] Step 1, preparation of Bacillus subtilis fermentation broth

[0055] 1. Take out the strain preservation tube, scratch the plate with LB solid medium for recovery, and incubate at 37°C for 48 hours. Pick a single colony under the plate and inoculate it into 50 ml of LB medium, and culture it in an incubator with shaking at 37°C for 24 hours. The seeds are inoculated with 5% inoculum amount, inoculated into a 5L large triangular flask with a liquid volume of 2L LB medium, cultured with shaking at 37°C for 20-28 hours, and the concentration of the bacteria solution is detected. The viable...

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Abstract

The present invention provides a composite microbial drink with high content of live bacteria, high activity, high stability, fast effect, no side effects, long storage time, obvious liver protection, anti-alcoholism, and anti-alcoholism. The composite microbial drink is prepared The raw materials are formed by mixing 30-40 parts of Bacillus subtilis fermentation broth, 30-40 parts of Thiobacillus peachum fermentation broth and 30-40 parts of Rhizopus oryzae fermentation broth in parts by weight. The selected bacterial strains of the present invention are all screened through the tests of anti-alcohol and ethanol retention rate. Higher ethanol retention rate can inhibit the absorption of ethanol in the gastrointestinal tract and accelerate the metabolism of ethanol in the stomach, thereby reducing the concentration of ethanol in the blood. Concentration; the compound microbial drink is rich in polyglutamic acid, peptides, polysaccharides and probiotics, which can effectively reduce serum ALT and AST levels, prevent alcoholic liver damage and improve liver antioxidant capacity to reduce liver damage.

Description

technical field [0001] The invention belongs to the technical field of microbial bacterial agents and food and drinks, and in particular relates to a compound microbial drink for relieving alcohol and protecting the liver and a preparation method thereof. Background technique [0002] In recent years, with the continuous improvement of people's living standards, the number of people drinking alcohol and the amount of alcohol drinking in my country are on the rise, and the number of people with alcohol (ethanol poisoning, especially acute alcohol poisoning) has increased sharply. The "Global Alcohol Policy Status Report" released by WHO in 2012 shows that, In the annual death toll in the world, nearly 4% of the deaths are caused by alcohol abuse, and about 2.5 million people die each year due to alcohol abuse. It can be seen that alcohol abuse has seriously endangered people's health, and may even cause a series of social problems Therefore, it is particularly important to find...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L2/38A23L33/00A01C1/00C12N1/20C12N1/14C12R1/125C12R1/01C12R1/69
CPCA01C1/00A23L2/382A23L33/00C12N1/14C12N1/20A23V2002/00A23V2200/334A23V2200/30A23V2250/206
Inventor 蒋常德蒋常银
Owner FOSHAN YANHUI BIOTECH CO LTD
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