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A kind of on-the-spot rapid detection method of sulfur dioxide in wine

A technology of sulfur dioxide and detection method, which is applied in the direction of material analysis, measuring device, color/spectral characteristic measurement through observation of the influence on chemical indicators, etc. It can solve the problems of long measurement time and low accuracy of measurement results, and achieve operation Simple, reduce the risk of false positive and false negative, fast and efficient carrying effect

Active Publication Date: 2020-12-08
SHANGHAI INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the Chinese patent (publication number CN 105241876A) detects sulfur dioxide in food by the pararosaniline hydrochloride colorimetric method, which requires complex sample pretreatment and takes a long time to measure; the Chinese patent (publication number CN 203101274 U) uses potassium iodide The colorimetric method of starch test paper is used to determine the residual sulfur dioxide. This method needs to build a device, and the accuracy of the measurement results is low.

Method used

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  • A kind of on-the-spot rapid detection method of sulfur dioxide in wine
  • A kind of on-the-spot rapid detection method of sulfur dioxide in wine
  • A kind of on-the-spot rapid detection method of sulfur dioxide in wine

Examples

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Embodiment 1

[0030] Embodiment 1: HS-PAD technology detects sulfur dioxide

[0031] (1) Preparation of reduced graphene

[0032] Utilize improved Hummers method to prepare graphene oxide, constant volume obtains 0.5mg / mL graphene oxide flocculation, take 5mL graphene oxide in the distillation flask, add 5 μ L hydrazine hydrate (35%) as reducing agent, add 100 μ L ammonia water (28 %), fully stirred, and refluxed at 95°C for 1h. Let stand at room temperature, centrifuge at 10000rpm for 20min, remove the lower layer and make up to volume to obtain a 0.5mg / mL reduced graphene solution.

[0033] (2) Prepare gold nanorods by seed crystal method:

[0034] a. Preparation of gold nano seed solution: at room temperature (25-28°C), prepare 9.75ml 0.1mol / L hexadecyltrimethylammonium bromide aqueous solution, stir evenly until transparent, add dropwise 0.25ml 0.01mol / L Chlorauric acid aqueous solution, after it is uniformly dispersed in the solution, quickly add 0.6mL freshly prepared 0.01mol / L sod...

Embodiment 2

[0040] Example 2: HS-PAD technology detects SO in wine 2

[0041] figure 1 Schematically provided the detection of SO in wine of the embodiment of the present application 2 The flow chart of described detection method comprises the following steps:

[0042] (1) prepare reduced graphene, step is with embodiment 1;

[0043] (2) Preparation and purification of gold nanorods, the steps are the same as in Example 1;

[0044] (3) Synthetic 4-mercaptopyridine (4-MPy) modified graphene / gold nanorod composite (AuNRs / rGO-MPy), the steps are the same as in Example 1;

[0045] (4) Build headspace sampling-analytical device (HS-PAD) based on paper, step is the same as embodiment 1;

[0046] (5) HS-PAD technology detects SO in wine 2 .

[0047] The wine samples used in the experiment were wine, white wine and rose wine purchased from supermarkets. When measuring sulfur dioxide in wine, 2mL wine sample is directly put into 10mL glass container for HS-PAD operation without any other p...

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Abstract

The invention discloses a method for rapidly detecting sulfur dioxide in wine on-site. The method comprises the following steps: (1) after placing a reduced graphene solution, 4-mercaptopyridine and agold nano-rod in a PBS (Phosphate Buffer solution) at room temperature, centrifuging and washing to obtain AuNRs / rGO-MPy; (2) filtering the AuNRs / rGO-MPy through an Anodisc filtering membrane to obtain an AuNRs / rGO-MPy filtering membrane; combining a Karl-Fisher reagent to construct a headspace sampling / paper-based analysis device; detecting sulfur dioxide by utilizing an ultraviolet spectrophotometer and a portable Raman spectrometer. The method disclosed by the invention has the advantages of simplicity and portability in operation, relatively rapid response, high selectivity and good sensitivity; a dual-sensing detection mode can realize optimal sensing performance of sulfur dioxide in a complicated matrix sample and the risks of false positive and false negative are reduced. Detectionlimits are as follows: a colorimetric method: 5.0mu M; an ultraviolet-visible spectrophotometry: 1.45mu M; SERS (Surface Enhanced Raman Scattering): 0.086mu M.

Description

technical field [0001] The application belongs to the technical field of food analysis and detection, and in particular relates to an on-site rapid detection method for sulfur dioxide in wine. Background technique [0002] Sulfur dioxide (SO 2 ) is widely used as a preservative in the food and beverage industry due to its excellent antioxidant and antibacterial properties, inhibiting the growth of bacteria and discoloration caused by oxidation. However, SO 2 Has many health risks, including inflammation and irritation of the skin and mucous membranes of the eyes, nose, throat and lungs. In addition, high concentrations of sulfur dioxide can affect lung function, trigger asthma, and aggravate underlying heart disease. According to EU statistics, SO in red wine and white wine 2 The maximum allowable concentrations of the two are 160mg / L and 210mg / L respectively. Therefore, it is urgent to establish the ultra-trace amount of SO in wine 2 method of detection. [0003] Sur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/65G01N21/33
CPCG01N21/33G01N21/658G01N21/78
Inventor 李丹段化珍马亚丹童璐子云王玲艳杜乐邓维
Owner SHANGHAI INST OF TECH
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