Application of dendrobium officinale polysaccharide in preparation of drug for treating osteoarthritis

A Dendrobium officinale polysaccharide and osteoarthritis technology, applied in the field of medicine, can solve the problems of high price of small molecule inhibitors, pending research on biological safety, irreversible osteoarthritis, etc., to protect the extracellular matrix of cartilage, relieve damage, The effect of alleviating cartilage degeneration

Active Publication Date: 2018-12-21
NANJING DRUM TOWER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the following technical problems exist in the traditional drug treatment methods: the drugs for the treatment of osteoarthritis provided by the prior art are limited to the relief of pain and other symptoms, irreversible early osteoarthritis disease process, and there are gastrointestinal reactions, kidney toxicity, etc. Multiple side effects that could cause other potential harm to the patient
However, the immunosuppressant is actually a recombinant active protein, the preparation method is complicated, the yield is low, and the storage conditions are high in the later stage.
However, small molecule inhibitors are expensive, their effects on other organs are unknown, and their biological safety needs to be studied

Method used

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  • Application of dendrobium officinale polysaccharide in preparation of drug for treating osteoarthritis
  • Application of dendrobium officinale polysaccharide in preparation of drug for treating osteoarthritis
  • Application of dendrobium officinale polysaccharide in preparation of drug for treating osteoarthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Dendrobium officinale polysaccharide extraction

[0031] Bioactive ingredients Dendrobium officinale polysaccharide, extracted from Dendrobium officinale. Remove the leaves of Dendrobium officinale and take the stems, dry in an oven at 80°C, grind into powder, accurately weigh 1.00g dry product coarse powder, wrap it with filter paper and degreasing gauze, and use petroleum ether (60-90°C) in a Soxhlet extractor Reflux and degrease in a water bath at 85°C for 1.5h, then reflux with 80% ethanol in a water bath at 95°C for 3h, take out the filter paper bag and dry it in a 60°C oven, then wash the sample in the filter paper into a 150ml round bottom flask with a narrow bottle, add 100ml Deionized water, extraction in boiling water twice, 3h / time, filter while hot, collect the filtrate, concentrate under reduced pressure on a rotary evaporator to 50ml, add a small amount of activated carbon for decolorization, filter, add 4 times the volume of the filtrate to mix with 95% e...

Embodiment 2

[0033] Dendrobium officinale polysaccharide preparation preparation:

[0034] Take by weighing 0.68g Dendrobium officinale polysaccharide, 2g citric acid and 0.8g sodium benzoate and mix, the mixture is dissolved in 100ml water for injection, water for injection is physiological saline, also can be replaced with phosphate buffer saline PBS (pH7.2), stir and mix Homogenize, filter impurities, and dispense into ampoules under sterile conditions.

Embodiment 3

[0036] Establishment of mouse medial meniscal instability (DMM) model:

[0037] Eight-week-old C57L / B6 mice were used for the experiment. After intraperitoneal anesthesia with chloral hydrate, the skin was routinely prepared to disinfect the knee joint. The right parapatella medial incision was taken to expose the knee joint, the joint capsule was cut, the joint cavity was opened, and the patella was dislocated laterally. , flex the knee joint as far as possible, expose the anterior cruciate ligament, cut the medial meniscus with a sharp knife under direct vision, remove the medial meniscus as much as possible, do not damage the cartilage surface during the operation, completely stop bleeding, reset the patella, and suture the incision layer by layer. The sham operation group was used as the control group, without any fixation measures after the operation, intramuscular injection of penicillin every day for 3 consecutive days. After 3 days, it was observed whether the knee joi...

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Abstract

The invention discloses application of dendrobium officinale polysaccharide in preparation of a drug for treating osteoarthritis. The dendrobium officinale polysaccharide is applied to the preparationof an osteoarthritis treating drug for inhibiting inflammatory cytokines IL-1beta and TNF-alpha, and degrading gene expression of matrix metalloproteinase 1, matrix metalloproteinase 3 and matrix metalloproteinase 13 of a cartilage extracellular matrix, and is also applied to the preparation of an osteoarthritis treating drug for promoting glycan expression of II-type collagen and poly-protein ofthe cartilage extracellular matrix. That the dendrobium officinale polysaccharide has a remarkable effect in the aspect of treating osteoarthritis and protecting cartilage is found at the first time,the dendrobium officinale polysaccharide has the anti-inflammation and antioxidant effect, and by clearing away free radical, active oxygen and active nitrogen, the activity of antioxidant enzyme isimproved to reduce apoptosis of cartilage cells and relieve the damage of oxidative stress to cartilage. Therefore, the dendrobium officinale polysaccharide can be used as an effective component, andis of important social and economic significance in preparation of the drug for treating osteoarthritis.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of dendrobium officinale polysaccharide in the preparation of medicine for treating osteoarthritis. Background technique [0002] Osteoarthritis (OA) is one of the most common degenerative diseases in the elderly. It progressively invades articular cartilage, bone and synovial tissue, leading to joint pain, deformity and dysfunction, thereby affecting the patient's mobility. Due to the complexity of the pathogenesis of OA, most of the current treatment methods for OA are aimed at relieving the symptoms of OA and relieving pain. Patients with severe middle and advanced OA can only choose joint replacement surgery in the end. [0003] Studies have found that inflammation and oxidative stress are closely related to the occurrence and development of OA. Compared with normal people, various inflammatory mediators in the joint tissues of OA patients, such as tumor nec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61K9/08A61P19/02A61P19/08A61P29/00A61P19/04
CPCA61K9/0019A61K9/08A61K31/715A61K47/12A61P19/02A61P19/04A61P19/08A61P29/00
Inventor 史冬泉蒋青吴睿徐兴全
Owner NANJING DRUM TOWER HOSPITAL
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