Rice brown planthopper harming inducible promoter and application thereof

Abstract

The invention discloses a rice brown planthopper harming inducible promoter and application thereof. The rice brown planthopper harming inducible promoter is named PAOS1, the length is 1889 bp, and the nucleotide sequence is as shown in SEQ ID NO:1. The promoter is the promoter of allene oxide synthase gene OsAOS1, namely the jasmonic acid (JA) key gene which is cloned and synthesized from the rice 'Zhonghuamedium flower 11' gene group, deficiency is conducted on the promoter PAOS1, 6 deficient segments with different lengths of 5' end are obtained, according to the research, elements expressing positively regulating affected by feeding specificity of brown planthopper exist in three regions, and meanwhile, the three regions are not activated by feeding induction of striped rice borer. Theinvention provides the seven promoters and the preparation method of the corresponding expression carriers and the genetic transformation method of rice mediated through agrobacterium, and the promoter features have the potential application value in breeding of rice insect resistant gene engineering.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to a rice brown planthopper damage-inducible promoter and application thereof. Background technique [0002] Rice is an important food crop. Its yield and planting area are second to that of corn. As one of many species, rice maintains the entire ecosystem and contributes to human well-being (Board M.Ecosystems and human well-being: synthesis.Washington, DC : World Resources Institute. 2005). Among the evolved species, most of them are not harmful to rice, and many species can improve the fertility and yield of rice, but a small number of species become rice pests, posing a threat to grain yield and quality, especially insects, which are harmful to rice during each growth period. cause some damages. 10% of annual rice yield loss is caused by insect pests (Chen H, Lin Y, Zhang Q. Review and prospect of transgenic rice research. Chin Sci Bull. 2009, 54: 4049–4068)....

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Problems solved by technology

At present, the research results of inducible promoters are more focused on abiotic stress induction (MishraRC, Grover A. Intergenic sequence between Arabidopsis caseinolytic protease B-cytoplasmic / heat shock protein100 and choline kinase genes functions as aheat-inducible bidirectional promoter. Plant Physiol. 2014,166:1646-58; Wang Jet al.Characterization of wheat TaSnRK2.7promoter in Arabidopsis.Planta.2018;Roy S et al.Functional analysis of light-regulated promoter region of AtPolλgene.Planta.2012,235:411-32 ), the results of biotic stress induction are more concentrated in plant diseases (Lin CH,ChenCY.The pathogen-inducible promoter of defense-related LsGRP1gene from Liliumfunctioning in phylogenetically distinct species of plants.Plant Sci.2017,254:22-31; P et al.Promoter elements of rice susceptibility genes are bound and activated by specific TAL effectors from the bacterial blightpathogen,Xanthomonas oryzae pv.oryzae.New Phytol.2010,187:1048-57; Vijayan Jet al.Cloning and functionally validation of ear Magnaporthe oryzaeresponsive CYP76M7promoter from rice.Front Plant Sci.2015,6:371), and there are few studies on insect feeding hazard-inducible promoters (Kumar M et al.Development of insect resistanttransgenic cotton lines expressing cry1EC gene from an insect bite and woundinducible promoter.J Biotechnol.2009,140:143-8;Siddique S et al.The promoter of a plant defensin gene directs specific expression in nematode-induced syncytia in Arabidopsis roots.Plant Physiol Biochem.2011,49:1100-7;Hua H etal.Analysis of rice genes induced by striped stemborer(Chilo suppressalis) attack identified a promoter fragment highly specifically responsive to insect feeding.Plant Mol Biol.2007,65:519-30)

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