Acrylic acid degrading bacterium and application thereof
A technology of acrylic acid and degrading bacteria, applied in the field of biological treatment of environmental pollutants, can solve the problems of high treatment cost, difficult control, heavy metal catalyst pollution, etc., and achieve the effects of high degradation efficiency and simple degradation conditions.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0040] Example 1: Isolation, purification and identification of Escherichia coli WX
[0041] 1. Isolation and purification of strains
[0042] Take the sludge from the acrylic acid wastewater treatment tank (located in Zhejiang Satellite Energy Co., Ltd.), let it stand for 24 hours, take 10mL and inoculate it into a 250mL culture bottle containing 100mL sterile water, shake and cultivate at 30°C for 30min at 160rpm, stop shaking and let stand 2min, inoculate 5mL of the suspension in 100mL acrylic acid liquid selection medium, 30°C, 160rpm shaking culture for 3d, after 3d, take 5mL suspension inoculation in 100mL fresh acrylic acid liquid selection medium, 30°C, 160rpm shaking culture for 3d , After 3 times of cultivation, use sterile water to prepare a certain concentration of bacterial liquid.
[0043] The obtained bacterial solution was separated and purified by streaking multiple times on acrylic acid solid selective medium to obtain a single colony, namely, an acrylic aci...
Embodiment 2
[0058] The fermentation culture of embodiment 2 escherichia coli WX
[0059] 1. Incline culture
[0060] Escherichia coli WX was inoculated into the slant medium, and cultured at 28-30°C for 3 days to obtain the bacterial slant;
[0061] Wherein, the final concentration of each component in the slant culture medium is: NH 4 Cl 1400~1500mg·L -1 , Na 2 HPO 4 5000~6000mg·L -1 , KH 2 PO 4 2800~3000mg·L -1 , NaCl900~1000mg·L -1 , yeast extract powder 1400~1500mg·L -1 , peptone 2800~3000mg·L -1 , acrylic acid 1000mg·L -1 , MgSO 4 ·7H 2 O 15~16mg·L -1 , CaCl 2 3.8~4mg·L -1 , CuSO 4 3.8~4mg·L -1 , FeSO4 3.8~4mg·L -1 , MnSO 4 3.8~4mg·L -1 , the solvent is water, the pH value is 7.0-7.5, and the agar is 18-20g·L -1 .
[0062] 2. Seed culture
[0063] Pick colonies from the slant of the bacteria and inoculate them into LB medium, culture at 28-30°C for 10-12 hours, and obtain seed liquid;
[0064] Wherein, the final concentration of each component in the seed ...
Embodiment 3
[0068] Example 3 Escherichia coli WX (Escherichia coli WX) acrylic acid degradation performance detection
[0069] 1. Investigate the performance of Escherichia coli WX to degrade acrylic acid under different initial pH conditions
[0070] The experiment of Escherichia coli WX degrading acrylic acid was carried out at different initial pHs, and the specific implementation steps were as follows:
[0071] With acrylic acid as carbon source (concentration 1000mg L -1 ), according to volume concentration 3% inoculum size will OD 600 Inoculate the 1.5-containing bacteria suspension (prepared by the method in Example 2) into acrylic acid liquid selection medium with different pH values (pH values are 4.0, 5.0, 6.0, 7.0, 8.0, 9.0), and culture at 30°C for 5 hours with shaking at 160rpm , to obtain the culture solution, the concentration of acrylic acid was determined by high performance liquid chromatography, that is, take 1.5mL culture solution as a sample, first centrifuge th...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More